Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,976,386	A→T	intergenic (‑1/‑213)	omrB ← / → galR	small regulatory RNA OmrB/DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,976,386	0	A	T	100.0%	191.7 / NA	56	intergenic (‑1/‑213)	omrB/galR	small regulatory RNA OmrB/DNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (27/29);  total (27/29)

AAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAA  >  NC_000913/2976251‑2976521                                                                                                                                        |                                                                                                                                        aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTcac                                                                                                                                     <  1:817126/140‑1 (MQ=255) aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTcac                                                                                                                                     >  2:74271/1‑140 (MQ=255)         cGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAg                                                                                                                             <  1:533821/140‑1 (MQ=255)          gAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGc                                                                                                                            <  1:575382/140‑1 (MQ=255)           aGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTcac                                                                                                                                     >  1:565158/1‑130 (MQ=255)           aGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTcac                                                                                                                                     <  2:565158/130‑1 (MQ=255)           aGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCa                                                                                                                           >  1:636245/1‑140 (MQ=255)               gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCt                                                                                                                       >  1:268967/1‑140 (MQ=255)                        gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGGGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAACCaaa                                                                                                              >  1:744812/1‑140 (MQ=255)                              tACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGccac                                                                                                        <  1:74271/140‑1 (MQ=255)                                      cAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                                                                                <  2:269750/140‑1 (MQ=255)                                         aTTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAAt                                                                                             >  1:121166/1‑140 (MQ=255)                                             atgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCa                                                                                         >  2:775829/1‑140 (MQ=255)                                              tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                                                                        <  1:432218/140‑1 (MQ=255)                                                  cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                                                                    >  2:607918/1‑140 (MQ=255)                                                  cGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTCGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                                                                    >  2:443576/1‑140 (MQ=255)                                                    gCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGt                                                                                  <  2:466508/140‑1 (MQ=255)                                                    gCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGt                                                                                  >  1:646045/1‑140 (MQ=255)                                                         aaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                                                                             <  2:828554/140‑1 (MQ=255)                                                         aaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                                                                             <  2:646045/140‑1 (MQ=255)                                                         aaaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                                                                             <  1:283377/140‑1 (MQ=255)                                                          aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                                                                            <  1:703777/140‑1 (MQ=255)                                                          aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                                                                            <  2:121166/140‑1 (MQ=255)                                                          aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                                                                            <  1:213034/140‑1 (MQ=255)                                                          aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                                                                            <  2:268967/140‑1 (MQ=255)                                                          aaaaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTCGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                                                                            <  1:443576/140‑1 (MQ=255)                                                                tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAgtgt                                                                      <  2:636245/140‑1 (MQ=255)                                                                          cgcAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGt                                                            >  2:685059/1‑140 (MQ=255)                                                                               gCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCa                                                       <  1:685059/140‑1 (MQ=255)                                                                                    tgtAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAAtctc                                                  >  2:458221/1‑140 (MQ=255)                                                                                         ttCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCa                                                       >  1:743717/1‑130 (MQ=255)                                                                                         ttCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCa                                                       <  2:743717/130‑1 (MQ=255)                                                                                         ttCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCttttt                                             >  1:771922/1‑140 (MQ=255)                                                                                         ttCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCttttt                                             <  1:458221/140‑1 (MQ=255)                                                                                          tCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCtttttt                                            <  1:801709/140‑1 (MQ=255)                                                                                             tgtgCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGt                                         >  1:80913/1‑140 (MQ=255)                                                                                              gtgCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTc                                        >  2:272743/1‑140 (MQ=255)                                                                                              gtgCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTc                                        >  2:62442/1‑140 (MQ=255)                                                                                                 cTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTctta                                     >  1:770462/1‑140 (MQ=255)                                                                                                 cTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTctt                                      >  2:322024/1‑139 (MQ=255)                                                                                                 cTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTctt                                      <  1:322024/139‑1 (MQ=255)                                                                                                      cccGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTc                                        <  2:667588/132‑1 (MQ=255)                                                                                                      cccGAAGTTGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTc                                        >  1:667588/1‑132 (MQ=255)                                                                                                              tGACTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGttt                        >  2:121296/1‑140 (MQ=255)                                                                                                                aCTTCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTgg                      >  1:785047/1‑140 (MQ=255)                                                                                                                  ttCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCt                    <  1:447574/140‑1 (MQ=255)                                                                                                                   tCACCTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTc                   >  1:156653/1‑140 (MQ=255)                                                                                                                      ccTATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTcaca                <  1:607918/140‑1 (MQ=255)                                                                                                                        tATCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCTCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACAtt              <  2:771922/140‑1 (MQ=255)                                                                                                                          tCAATACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTcc            >  2:613011/1‑140 (MQ=255)                                                                                                                             aTACCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCAc         <  1:775829/140‑1 (MQ=255)                                                                                                                               aCCTCTGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGa       >  1:547528/1‑140 (MQ=255)                                                                                                                                 ctctGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATg     >  2:22244/1‑140 (MQ=255)                                                                                                                                   ctGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGaa   <  1:613011/140‑1 (MQ=255)                                                                                                                                    tGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGaaa  <  2:770462/140‑1 (MQ=255)                                                                                                                                    tGGGTTCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGaaa  >  2:305664/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        AAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAATCTCTTTTTTTGTCTTACTTATTTGCGTTTGGCTCACATTCCCACGATGAAA  >  NC_000913/2976251‑2976521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑