Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,207	A→G	T50A (ACC→GCC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,207	0	A	G	100.0%	185.0 / NA	54	T50A (ACC→GCC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

ATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAA  >  NC_000913/3016072‑3016343                                                                                                                                        |                                                                                                                                         atGCCTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      <  2:762931/140‑1 (MQ=255)     gTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGcc                                                                                                                                  >  2:715755/1‑140 (MQ=255)     gTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGcc                                                                                                                                  >  1:507290/1‑140 (MQ=255)      tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      >  2:42542/1‑135 (MQ=255)      tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCtg                                                                                                                                      <  1:42542/135‑1 (MQ=255)      tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCa                                                                                                                                 <  2:507290/140‑1 (MQ=255)      tCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCa                                                                                                                                 >  2:565977/1‑140 (MQ=255)       cccATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCAc                                                                                                                                >  1:721205/1‑140 (MQ=255)        ccATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACt                                                                                                                               <  2:792715/140‑1 (MQ=255)         cATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTc                                                                                                                              >  1:463290/1‑140 (MQ=255)                    aGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCt                                                                                                                   >  1:348957/1‑140 (MQ=255)                          ttttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCt                                                                                                             >  1:23061/1‑140 (MQ=255)                          ttttGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCt                                                                                                             <  2:608212/140‑1 (MQ=255)                             tGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGc                                                                                                          <  2:23061/140‑1 (MQ=255)                                cgcgCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       <  2:348957/140‑1 (MQ=255)                                   gcATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGcac                                                                                                    >  2:572413/1‑140 (MQ=255)                                    cATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGcaca                                                                                                   >  2:557625/1‑140 (MQ=255)                                               aaTACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTcgcg                                                                                        <  2:463290/140‑1 (MQ=255)                                                 tACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       >  1:192477/1‑123 (MQ=255)                                                 tACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCg                                                                                                       <  2:192477/123‑1 (MQ=255)                                                  aCCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCaa                                                                                     <  1:599157/140‑1 (MQ=255)                                                      acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  1:508346/1‑140 (MQ=255)                                                      acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  1:348833/1‑140 (MQ=255)                                                      acaacaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAata                                                                                 >  2:585584/1‑140 (MQ=255)                                                         acaacGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTg                                                                              >  2:279605/1‑140 (MQ=255)                                                            acGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCa                                                                           >  2:517310/1‑140 (MQ=255)                                                              gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         >  2:28037/1‑140 (MQ=255)                                                              gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         <  1:734126/140‑1 (MQ=255)                                                              gCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACt                                                                         >  2:734126/1‑140 (MQ=255)                                                                    tCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTgg                                                                   >  1:95288/1‑140 (MQ=255)                                                                        tGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGa                                                               <  1:517310/140‑1 (MQ=255)                                                                        tGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGa                                                               <  2:508346/140‑1 (MQ=255)                                                                              tCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCg                                                         >  1:665399/1‑140 (MQ=255)                                                                              tCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCg                                                         <  2:512841/140‑1 (MQ=255)                                                                                  gAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACg                                                     >  1:324241/1‑140 (MQ=255)                                                                                             ttACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAAc                                          >  2:680293/1‑140 (MQ=255)                                                                                                     cTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAcc                                  >  2:301370/1‑140 (MQ=255)                                                                                                               tAAAACTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCTGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTc                        >  2:719423/1‑140 (MQ=255)                                                                                                                aaaaCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc                                   <  1:202900/128‑1 (MQ=255)                                                                                                                aaaaCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAAc                                   >  2:202900/1‑128 (MQ=255)                                                                                                                   aCTGTTAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    <  2:665399/140‑1 (MQ=255)                                                                                                                       ttAGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAAGTAAAAACCGTCCAAATTCTTGACCGc                >  1:580812/1‑140 (MQ=255)                                                                                                                         aGCGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCt              <  2:348833/140‑1 (MQ=255)                                                                                                                           cGTCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTgg            <  1:565977/140‑1 (MQ=255)                                                                                                                             tCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTg             >  2:661252/1‑137 (MQ=255)                                                                                                                             tCTTCGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTg             <  1:661252/137‑1 (MQ=255)                                                                                                                                 cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    >  2:219995/1‑126 (MQ=255)                                                                                                                                 cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGa                    <  1:219995/126‑1 (MQ=255)                                                                                                                                 cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTgg      >  1:183694/1‑140 (MQ=255)                                                                                                                                 cGGTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTgg      >  2:808440/1‑140 (MQ=255)                                                                                                                                   gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  2:580812/140‑1 (MQ=255)                                                                                                                                   gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  1:301370/140‑1 (MQ=255)                                                                                                                                   gTGAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaa    <  2:343754/140‑1 (MQ=255)                                                                                                                                     gAAGCCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGaaaa  <  1:28037/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ATGCGTCCCATTCCGTTTGAGGAACTTTTGACGCGCATATTTGATGAATACCAACAACAACGCTCAATCTTTGGTATTCCCGAGCAACAGTTTTACTCACCTGTAAAAGGTAAAACTGTTAGCGTCTTCGGTGAAACCTGTGCCACTCCCGTCGGCCCTGCCGCTGGCCCGCACACGCAGCTCGCGCAAAATATTGTCACTTCCTGGCTGACTGGCGGACGCTTCATCGAACTAAAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAA  >  NC_000913/3016072‑3016343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑