Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,157	T→C	intergenic (‑77/+16)	yggE ← / ← argO	uncharacterized protein YggE/L‑arginine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,157	0	T	C	100.0%	141.8 / NA	41	intergenic (‑77/+16)	yggE/argO	uncharacterized protein YggE/L‑arginine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/18);  total (23/18)

TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292                                                                                                                                        |                                                                                                                                        tGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCa                                                                                                                                     <  2:157622/140‑1 (MQ=255)       gCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGAt                                                                                                                               <  1:37744/140‑1 (MQ=255)           tCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           <  1:641157/140‑1 (MQ=255)             ccGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGcc                                                                                                                         >  2:276014/1‑140 (MQ=255)                  aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACt                                                                                                                    >  2:175861/1‑140 (MQ=255)                  aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACt                                                                                                                    >  1:542043/1‑140 (MQ=255)                       ccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACa                                                                                                               <  2:542043/140‑1 (MQ=255)                         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                             >  1:795158/1‑140 (MQ=255)                            aaTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCt                                                                                                          <  2:795158/140‑1 (MQ=255)                                 cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTTCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     >  1:653830/1‑140 (MQ=255)                                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCAt                                                                                                   >  1:186337/1‑140 (MQ=255)                                     aGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGa                                                                                                 >  2:166384/1‑140 (MQ=255)                                        gCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCa                                                                                              >  2:389321/1‑140 (MQ=255)                                            tAACTTTGAACTCCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                          <  1:248752/140‑1 (MQ=255)                                                 ttGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtct                                                                                     >  1:428376/1‑140 (MQ=255)                                                        tCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCa                                                                              >  1:673448/1‑140 (MQ=255)                                                         cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAg                                                                             >  2:229976/1‑140 (MQ=255)                                                              tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                        <  1:621564/140‑1 (MQ=255)                                                                 tctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAgg                                                                     >  1:328649/1‑140 (MQ=255)                                                                     tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                       <  1:670852/134‑1 (MQ=255)                                                                     tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                       >  2:670852/1‑134 (MQ=255)                                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                >  1:69161/1‑140 (MQ=255)                                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  2:673448/140‑1 (MQ=255)                                                                          gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                <  1:473399/136‑1 (MQ=255)                                                                          gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                >  2:473399/1‑136 (MQ=255)                                                                           ttAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACca                                                           <  2:265880/140‑1 (MQ=255)                                                                           ttAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACca                                                           >  1:676447/1‑140 (MQ=255)                                                                                  aCTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                    <  1:389321/140‑1 (MQ=255)                                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   <  1:166384/140‑1 (MQ=255)                                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGAAAGGCAATAAACCACATAacac                                                   <  1:175861/140‑1 (MQ=255)                                                                                     tGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACAt                                                 >  2:638788/1‑140 (MQ=255)                                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               <  2:374395/140‑1 (MQ=255)                                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               >  1:463948/1‑140 (MQ=255)                                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                         <  2:69161/140‑1 (MQ=255)                                                                                                 aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGa                                     >  2:817078/1‑140 (MQ=255)                                                                                                                aaGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                      >  2:825512/1‑140 (MQ=255)                                                                                                                         gcCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc             <  1:825512/140‑1 (MQ=255)                                                                                                                            tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTg          >  2:615154/1‑140 (MQ=255)                                                                                                                             ctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCGCGCCAGCTGCAAGGCAATAAACCACATAATACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTgc         >  1:725357/1‑140 (MQ=255)                                                                                                                                   tATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAc   >  1:440084/1‑140 (MQ=255)                                                                                                                                    aTTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg  <  1:638788/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑