Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,907	G→A	W51* (TGG→TGA)	yggI →	conserved protein YggI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,907	0	G	A	100.0%	204.2 / NA	58	W51* (TGG→TGA)	yggI	conserved protein YggI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

GCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCA  >  NC_000913/3089779‑3090043                                                                                                                                 |                                                                                                                                         gCCATCCAACAGGCCGTTGTGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTa                                                                                                                               >  1:657062/1‑140 (MQ=255)     tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  2:459986/1‑140 (MQ=255)     tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  1:264253/1‑140 (MQ=255)     tCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGAAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGaa                                                                                                                           >  2:566416/1‑140 (MQ=255)        aaCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAAtt                                                                                                                        <  1:231093/140‑1 (MQ=255)        aaCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAAtt                                                                                                                        <  2:479926/140‑1 (MQ=255)         aCAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTc                                                                                                                       >  1:581466/1‑140 (MQ=255)            ggCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGcc                                                                                                                    <  1:765488/140‑1 (MQ=255)               cGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCa                                                                                                                 >  2:314574/1‑140 (MQ=255)                       gTCGCCTGCGGGGAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGtt                                                                                                         >  2:708594/1‑140 (MQ=255)                           ccTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  1:566416/140‑1 (MQ=255)                           ccTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  2:792676/140‑1 (MQ=255)                               cGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGtt                                                                                                 >  2:169448/1‑140 (MQ=255)                                   aaaaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGaa                                                                                             <  2:581466/140‑1 (MQ=255)                                     aaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     <  2:471611/130‑1 (MQ=255)                                     aaaCTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGc                                                                                                     >  1:471611/1‑130 (MQ=255)                                         tCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGt                                                                                       <  2:51023/140‑1 (MQ=255)                                          cGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTg                                                                                      <  1:576357/140‑1 (MQ=255)                                                 gCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCttt                                                                               <  1:459986/140‑1 (MQ=255)                                                   cAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTa                                                                             <  2:469661/140‑1 (MQ=255)                                                     aCCTGAAGCTAGGGCGTAACTGCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTAtt                                                                           <  2:483065/140‑1 (MQ=255)                                                      ccTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTg                                                                          >  2:629336/1‑140 (MQ=255)                                                       cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  2:264253/140‑1 (MQ=255)                                                       cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  1:437694/140‑1 (MQ=255)                                                       cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  1:329116/140‑1 (MQ=255)                                                       cTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTga                                                                         <  2:433400/140‑1 (MQ=255)                                                             cTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGt                                                                   >  2:553436/1‑140 (MQ=255)                                                              tAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTg                                                                  <  1:629336/140‑1 (MQ=255)                                                               aGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTgg                                                                 >  2:495281/1‑140 (MQ=255)                                                                gggCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGt                                                                >  1:525654/1‑140 (MQ=255)                                                                gggCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCATCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGt                                                                >  2:309659/1‑140 (MQ=255)                                                                          aCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTgaag                                                                       >  2:145597/1‑123 (MQ=255)                                                                          aCCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTgaag                                                                       <  1:145597/123‑1 (MQ=255)                                                                           cccGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAAc                                                     >  1:71812/1‑140 (MQ=255)                                                                             cGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTg                                                   <  2:71812/140‑1 (MQ=255)                                                                                aGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGca                                                >  2:488746/1‑140 (MQ=255)                                                                                 gCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGcac                                               >  1:417719/1‑140 (MQ=255)                                                                                  ccAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGcaca                                              <  2:525654/140‑1 (MQ=255)                                                                                        ctctctTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        <  2:417719/140‑1 (MQ=255)                                                                                         tctctTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTg                                       <  1:154013/140‑1 (MQ=255)                                                                                                cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        >  2:16209/1‑132 (MQ=255)                                                                                                cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCt                                        <  1:16209/132‑1 (MQ=255)                                                                                                cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa                                <  1:537134/140‑1 (MQ=255)                                                                                                cacCCAGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGa                                >  1:183949/1‑140 (MQ=255)                                                                                                     aGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt                           >  2:313966/1‑140 (MQ=255)                                                                                                     aGCGCGGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAt                           >  2:114064/1‑140 (MQ=255)                                                                                                       cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         >  1:779003/1‑140 (MQ=255)                                                                                                       cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         <  1:708594/140‑1 (MQ=255)                                                                                                       cgcgGAACCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTACTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACAttt                         >  1:770942/1‑140 (MQ=255)                                                                                                            aaCCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAACCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGcc                    >  2:212698/1‑140 (MQ=255)                                                                                                             aCCTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAACCAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcg                   <  1:212698/140‑1 (MQ=255)                                                                                                               cTCCGCCGGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCcgcg                 <  2:267484/140‑1 (MQ=255)                                                                                                                    ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:313966/140‑1 (MQ=255)                                                                                                                    ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:314574/140‑1 (MQ=255)                                                                                                                    ccgGAACGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAgcg            <  1:488746/140‑1 (MQ=255)                                                                                                                        aaCGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTACTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCcac        <  2:770942/140‑1 (MQ=255)                                                                                                                          cGGCCTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACAt      >  2:65977/1‑140 (MQ=255)                                                                                                                             ccTGACTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGc   >  2:576197/1‑140 (MQ=255)                                                                                                                              ctgactggAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCa  <  2:623214/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         GCCATCCAACAGGCCGTTATGCGTCGCCTGCGGGAAAAACTCGCCCAGGCCAACCTGAAGCTAGGGCGTAACTACCCGGAGCCAAAACTCTCTTACACCCAGCGCGGAACCTCCGCCGGAACGGCCTGGCTGGAAAGCTATGAAATTCGCCTCAATCCCGTTTTGCTGTTGGAAAACAGTGAAGCTTTTATTGAAGAAGTGGTACCGCACGAACTGGCACATTTGCTGGTATGGAAACATTTCGGCCGCGTAGCGCCACATGGCA  >  NC_000913/3089779‑3090043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑