Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,636	T→C	intergenic (‑71/+46)	yggM ← / ← ansB	DUF1202 domain‑containing protein YggM/L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,636	0	T	C	100.0%	152.9 / NA	44	intergenic (‑71/+46)	yggM/ansB	DUF1202 domain‑containing protein YggM/L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

TCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTT  >  NC_000913/3099501‑3099771                                                                                                                                        |                                                                                                                                        tCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGt                                                                                                                                     >  2:327950/1‑140 (MQ=255) tCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGt                                                                                                                                     <  2:579815/140‑1 (MQ=255)    gCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTg                                                                                                                                  >  2:726676/1‑140 (MQ=255)     cccAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGa                                                                                                                                 >  1:489997/1‑140 (MQ=255)         ggCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTg                                                                                                                             >  1:200838/1‑140 (MQ=255)            aTTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGaaa                                                                                                                          <  1:690996/140‑1 (MQ=255)               aCCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAg                                                                                                                       >  2:429688/1‑140 (MQ=255)                 cAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGcc                                                                                                                     <  1:726676/140‑1 (MQ=255)                   gTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGcccc                                                                                                                   <  2:47571/140‑1 (MQ=255)                     catcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCgg                                                                                                                 >  2:504509/1‑140 (MQ=255)                       tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:636871/140‑1 (MQ=255)                       tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:686285/140‑1 (MQ=255)                       tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:660327/140‑1 (MQ=255)                       tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:647521/140‑1 (MQ=255)                       tcaACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTCGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  2:734217/140‑1 (MQ=255)                        caACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCAc                                                                                                              >  2:517902/1‑140 (MQ=255)                              aaGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATAcc                                                                                                        >  1:485356/1‑140 (MQ=255)                                     gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  1:477316/1‑140 (MQ=255)                                     gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  2:344028/1‑140 (MQ=255)                                     gccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcga                                                                                                 >  1:270670/1‑140 (MQ=255)                                      ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  2:779965/140‑1 (MQ=255)                                      ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                >  2:567049/1‑140 (MQ=255)                                      ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  2:369232/140‑1 (MQ=255)                                      ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  1:563859/140‑1 (MQ=255)                                      ccgtgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGggcgag                                                                                                <  1:336915/140‑1 (MQ=255)                                         tgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               <  1:728714/122‑1 (MQ=255)                                         tgccAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCa                                                                                                               >  2:728714/1‑122 (MQ=255)                                                aaTCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTa                                                                                      <  2:665228/140‑1 (MQ=255)                                                           ttttCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAga                                                                           <  1:504509/140‑1 (MQ=255)                                                              tCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAgatct                                                                        <  2:618758/140‑1 (MQ=255)                                                                aCCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAgatctgc                                                                      <  2:485356/140‑1 (MQ=255)                                                                             cACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCg                                                         <  1:327950/140‑1 (MQ=255)                                                                                         tttATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGttt                                             <  1:205148/140‑1 (MQ=255)                                                                                              tCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTc                                        >  1:587790/1‑140 (MQ=255)                                                                                                cGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCGGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCag                                      >  2:740509/1‑140 (MQ=255)                                                                                                  gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATNTTTGGNNNGCNTCagag                                    <  2:477316/140‑1 (MQ=255)                                                                                                  gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    >  1:805570/1‑140 (MQ=255)                                                                                                  gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    <  1:740509/140‑1 (MQ=255)                                                                                                  gTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGCCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCagag                                    >  2:427284/1‑140 (MQ=255)                                                                                                   tGATTATCATTAGGGTAATTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGc                                   >  2:229006/1‑140 (MQ=255)                                                                                                                   aaTTAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcg                   >  2:816322/1‑140 (MQ=255)                                                                                                                     ttAAAATTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAAcgcg                 >  2:665358/1‑140 (MQ=255)                                                                                                                          aTTGGCAATGGACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCtt            >  1:702124/1‑140 (MQ=255)                                                                                                                                    gACGCGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAAAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGtt  <  2:200838/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCTGCCCAGGCATTACCAGTCATCAACATAAGCAGTGCCGTGCCAACAATCCATTGCTTTTTCACCCGAAAAACCTCACGCGGAATAATTTATTCCGGTGATTATCATTAGGGTAATTAAAATTGGCAATGGACGTGAGTCTGAAGTGAAAAAGCCCCGGCACGATACCGGGGCGAGGCGATTAGTACTGATTGAAGATCTGCTGGATCTGCTGCGGATCTTTGGTTTGCGTCAGAGCCAGTTGCAGCAGAACGCGCGCTTTTTGCGGGTT  >  NC_000913/3099501‑3099771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑