Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,193,431	A→G	T190A (ACC→GCC)	yqiK →	protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,431	0	A	G	100.0%	211.4 / NA	60	T190A (ACC→GCC)	yqiK	protein YqiK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/30);  total (30/30)

CGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAA  >  NC_000913/3193300‑3193562                                                                                                                                    |                                                                                                                                    cgcAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGaaa                                                                                                                             >  2:453550/1‑140 (MQ=255)        gACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACAtt                                                                                                                      >  1:757178/1‑140 (MQ=255)        gACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACAtt                                                                                                                      <  1:45634/140‑1 (MQ=255)          ccATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTc                                                                                                                    <  2:757178/140‑1 (MQ=255)                    gTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACa                                                                                                          <  1:112270/140‑1 (MQ=255)                       aCAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATg                                                                                                       >  2:519034/1‑140 (MQ=255)                        cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGc                                                                                                      >  2:595706/1‑140 (MQ=255)                        cAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGc                                                                                                      >  1:33649/1‑140 (MQ=255)                             tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACa                                                                                                          <  1:111360/131‑1 (MQ=255)                             tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACa                                                                                                          >  2:111360/1‑131 (MQ=255)                             tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCttt                                                                                                  <  2:437455/139‑1 (MQ=255)                             tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCttt                                                                                                  >  1:437455/1‑139 (MQ=255)                             tACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTg                                                                                                 >  2:94263/1‑140 (MQ=255)                              aCCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGa                                                                                                >  1:189979/1‑140 (MQ=255)                              aCCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGa                                                                                                >  1:600/1‑140 (MQ=255)                              aCCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGa                                                                                                >  2:200770/1‑140 (MQ=255)                               cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGAc                                                                                               >  1:595742/1‑140 (MQ=255)                               cccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGAc                                                                                               >  1:629614/1‑140 (MQ=255)                                ccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAAt                                                                                                        <  1:290036/130‑1 (MQ=255)                                ccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAAt                                                                                                        >  2:290036/1‑130 (MQ=255)                                ccGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACg                                                                                              >  2:322137/1‑140 (MQ=255)                                  gcgAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGcc                                                                                            >  1:458683/1‑140 (MQ=255)                                   cgAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCg                                                                                           <  2:600/140‑1 (MQ=255)                                   cgAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCg                                                                                           <  2:458683/140‑1 (MQ=255)                                             gtgCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAAc                                                                                 <  2:29373/140‑1 (MQ=255)                                                  gggggTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATATCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAAc                                                                            <  1:602315/140‑1 (MQ=255)                                                        gCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGtt                                                                                     <  1:301920/125‑1 (MQ=255)                                                        gCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGtt                                                                                     >  2:301920/1‑125 (MQ=255)                                                             aTACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGaa                                                                 >  1:823307/1‑140 (MQ=255)                                                              tACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGaaa                                                                <  2:602705/140‑1 (MQ=255)                                                                   tGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGAc                                                                        <  1:281554/127‑1 (MQ=255)                                                                   tGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGAc                                                                        >  2:281554/1‑127 (MQ=255)                                                                     gCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAgcg                                                         >  1:438144/1‑140 (MQ=255)                                                                      cAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAgcgc                                                        >  1:171764/1‑140 (MQ=255)                                                                       agaagaCCTGTCGAAAAACGGTCTGGAACTGGTGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGcc                                                       <  2:33649/140‑1 (MQ=255)                                                                              cTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTcgccg                                                >  2:77470/1‑140 (MQ=255)                                                                               tGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTcgccgc                                               >  1:272676/1‑140 (MQ=255)                                                                                gTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCcgcg                                              <  1:200770/140‑1 (MQ=255)                                                                                      aaaCGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTa                                        <  2:595742/140‑1 (MQ=255)                                                                                       aaCGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTaa                                       <  2:189979/140‑1 (MQ=255)                                                                                              tGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGtt                                >  2:253674/1‑140 (MQ=255)                                                                                              tGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGtt                                <  1:595706/140‑1 (MQ=255)                                                                                                 aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             <  1:653183/140‑1 (MQ=255)                                                                                                 aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             <  1:519034/140‑1 (MQ=255)                                                                                                 aaCTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             <  2:158761/140‑1 (MQ=255)                                                                                                   cTGGAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACa                           <  1:253674/140‑1 (MQ=255)                                                                                                     ggAGAGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCTAACGTAACGAAGTTGAACAgg                         <  2:561186/140‑1 (MQ=255)                                                                                                      gagaGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             <  1:804600/135‑1 (MQ=255)                                                                                                      gagaGCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             >  2:804600/1‑135 (MQ=255)                                                                                                         agCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACa                           >  2:511327/1‑134 (MQ=255)                                                                                                         agCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACa                           <  1:511327/134‑1 (MQ=255)                                                                                                         agCGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGt                     <  1:322137/140‑1 (MQ=255)                                                                                                           cGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             >  2:537476/1‑130 (MQ=255)                                                                                                           cGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGaa                             <  1:537476/130‑1 (MQ=255)                                                                                                           cGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAg                   <  1:94263/140‑1 (MQ=255)                                                                                                           cGTTTCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAg                   <  2:171764/140‑1 (MQ=255)                                                                                                             tttCACTTACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGaa                 >  1:253557/1‑140 (MQ=255)                                                                                                                    tACCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCgg          >  1:258627/1‑140 (MQ=255)                                                                                                                     aCCAACTTTAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGt         >  1:62289/1‑140 (MQ=255)                                                                                                                            ttAACCAGGCCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGaa  <  2:253557/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CGCAAATGACCATGCATGAGTTACAGGATACCCGCGAGAACTTTGTGCAGGGGGTGCAAAATACAGTGGCAGAAGACCTGTCGAAAAACGGTCTGGAACTGGAGAGCGTTTCACTTACCAACTTTAACCAGACCTCGAAAGAACATTTCAATCCGAACAATGCCTTTGACGCCGAAGGTTTAACCAAACTGACTCAGGAAACAGAGCGCCGTCGCCGCGAACGTAACGAAGTTGAACAGGATGTAGAAGTTGCGGTGCGTGAA  >  NC_000913/3193300‑3193562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑