Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,200	A→G	K323E (AAA→GAA)	ygjI →	putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,200	0	A	G	100.0%	275.5 / NA	78	K323E (AAA→GAA)	ygjI	putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/45);  total (33/45)

TGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA  >  NC_000913/3227064‑3227335                                                                                                                                         |                                                                                                                                        tGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGgaaa                                                                                                                                      >  1:659492/1‑140 (MQ=255)    tCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCg                                                                                                                                   <  2:825004/140‑1 (MQ=255)    tCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCg                                                                                                                                   >  1:426448/1‑140 (MQ=255)      tACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTc                                                                                                                                 >  1:458731/1‑140 (MQ=255)       aCCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCg                                                                                                                                >  2:717340/1‑140 (MQ=255)         cGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGaa                                                                                                                              <  1:608203/140‑1 (MQ=255)                  tgatgaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTTGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGaa                                                                                                                     >  2:739733/1‑140 (MQ=255)                   gatgaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGaaa                                                                                                                    <  1:310683/140‑1 (MQ=255)                      gaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAAc                                                                                                                             <  2:495848/128‑1 (MQ=255)                      gaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAAc                                                                                                                             >  1:495848/1‑128 (MQ=255)                         tCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCg                                                                                                              >  2:570513/1‑140 (MQ=255)                         tCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCg                                                                                                              >  2:287936/1‑140 (MQ=255)                         tCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCg                                                                                                              >  1:596421/1‑140 (MQ=255)                               tGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCgg                                                                                                        >  2:171740/1‑140 (MQ=255)                                 tCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcg                                                                                                      >  2:793730/1‑140 (MQ=255)                                      cTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcgc                                                                                                 <  1:784279/140‑1 (MQ=255)                                      cTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcgc                                                                                                 <  1:793730/140‑1 (MQ=255)                                         gtgtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGc                                                                                              <  2:543630/140‑1 (MQ=255)                                           gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGt                                                                                            >  1:147674/1‑140 (MQ=255)                                           gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGt                                                                                            <  2:659492/140‑1 (MQ=255)                                           gtCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGt                                                                                            >  2:34949/1‑140 (MQ=255)                                              cTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGa                                                                                         <  2:396793/140‑1 (MQ=255)                                                ttACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATc                                                                                       <  2:773272/140‑1 (MQ=255)                                                ttACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATc                                                                                       <  2:458731/140‑1 (MQ=255)                                                   ccGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGa                                                                                         <  1:486980/135‑1 (MQ=255)                                                   ccGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGa                                                                                         >  2:486980/1‑135 (MQ=255)                                                    cgcgATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGt                                                                                   <  1:251590/140‑1 (MQ=255)                                                    cgcgATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGt                                                                                   >  1:701121/1‑140 (MQ=255)                                                      cgATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcg                                                                                                  <  2:764190/123‑1 (MQ=255)                                                      cgATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGgcgcgcg                                                                                                  >  1:764190/1‑123 (MQ=255)                                                        aTGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCt                                                                               >  2:809671/1‑140 (MQ=255)                                                         tGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTg                                                                              >  2:700981/1‑140 (MQ=255)                                                         tGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTg                                                                              >  1:316714/1‑140 (MQ=255)                                                              ggTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGt                                                                         <  2:426448/140‑1 (MQ=255)                                                                   ccTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATcc                                                                    >  1:361665/1‑140 (MQ=255)                                                                     tgctgATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCg                                                                  >  2:204682/1‑140 (MQ=255)                                                                     tgctgATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCg                                                                  >  1:729174/1‑140 (MQ=255)                                                                     tgctgATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCg                                                                  >  2:495488/1‑140 (MQ=255)                                                                          aTGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCtgat                                                             >  1:793397/1‑140 (MQ=255)                                                                             tgGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTgatgat                                                          <  1:540776/140‑1 (MQ=255)                                                                               gACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTa                                                        >  1:528093/1‑140 (MQ=255)                                                                                    cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                >  1:254028/1‑127 (MQ=255)                                                                                    cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                <  2:254028/127‑1 (MQ=255)                                                                                    cAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCg                                                   >  2:813660/1‑140 (MQ=255)                                                                                     aaCGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGa                                                  >  2:124074/1‑140 (MQ=255)                                                                                       cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                <  2:378924/124‑1 (MQ=255)                                                                                       cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                >  1:664822/1‑124 (MQ=255)                                                                                       cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                >  1:378924/1‑124 (MQ=255)                                                                                       cGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCt                                                                <  2:664822/124‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:204682/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  2:596421/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  2:701121/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:570513/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:700981/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  2:361665/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:287936/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:809671/140‑1 (MQ=255)                                                                                          cGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:717340/140‑1 (MQ=255)                                                                                           ggTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                            <  1:171740/140‑1 (MQ=255)                                                                                            gTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                           >  1:534753/1‑140 (MQ=255)                                                                                                    tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGTAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                   <  1:37294/140‑1 (MQ=255)                                                                                                    tttCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                   <  1:813660/140‑1 (MQ=255)                                                                                                        ttctCCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGt                               <  1:739733/140‑1 (MQ=255)                                                                                                         tctcCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             >  2:313971/1‑125 (MQ=255)                                                                                                         tctcCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                             <  1:313971/125‑1 (MQ=255)                                                                                                          ctcCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGc                             <  2:793397/140‑1 (MQ=255)                                                                                                          ctcCGAAATCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGc                             <  1:124074/140‑1 (MQ=255)                                                                                                                  tCCCGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtg                     <  1:495488/140‑1 (MQ=255)                                                                                                                    ccGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                   <  2:316714/140‑1 (MQ=255)                                                                                                                    ccGGAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                   <  1:240866/140‑1 (MQ=255)                                                                                                                       gAAGGCATCTTTGGTAAGGAAACCGTCGAACTTAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                <  2:293042/140‑1 (MQ=255)                                                                                                                       gAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                <  2:147674/140‑1 (MQ=255)                                                                                                                       gAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                <  1:452940/140‑1 (MQ=255)                                                                                                                       gAAGGCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACCGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                <  2:774226/140‑1 (MQ=255)                                                                                                                          ggCATCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCACAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc             <  1:310240/140‑1 (MQ=255)                                                                                                                              tCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                <  2:382711/133‑1 (MQ=255)                                                                                                                              tCTTTGGTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                >  1:382711/1‑133 (MQ=255)                                                                                                                                    gTAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat   <  2:534753/140‑1 (MQ=255)                                                                                                                                     tAAGGAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata  >  1:200755/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGGTCTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAAGGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA  >  NC_000913/3227064‑3227335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑