Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,675	C→T	D283N (GAT→AAT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,675	0	C	T	100.0%	130.7 / NA	38	D283N (GAT→AAT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

CACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAG  >  NC_000913/73559‑73803                                                                                                                     |                                                                                                                                 cACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTgca                                                                                                           >  2:94129/1‑140 (MQ=255) cACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTgca                                                                                                           >  1:55535/1‑140 (MQ=255)         gCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg                                                                                                   <  2:55535/140‑1 (MQ=255)              tGGCGATTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTgcag                                                                                                          <  1:579738/128‑1 (MQ=255)              tGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTgcag                                                                                                          >  2:579738/1‑128 (MQ=255)                 ggttttCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg                                                                                           <  2:719587/137‑1 (MQ=255)                      tcccccACCGACGACCACCGCCAGTAACGGTAGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa                                                                                      <  1:453244/138‑1 (MQ=255)                      ccccccATCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa                                                                                      <  2:108004/138‑1 (MQ=255)                          ccATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGc                                                                                  >  1:155370/1‑140 (MQ=255)                            aTCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCt                                                                                <  2:155370/140‑1 (MQ=255)                                          ccAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGa                                                                  <  2:361124/140‑1 (MQ=255)                                           cAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGAc                                                                 <  1:376184/140‑1 (MQ=255)                                           cAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGAc                                                                 <  2:135416/140‑1 (MQ=255)                                              tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCa                                                              >  1:267825/1‑140 (MQ=255)                                                      gcagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAcc                                                      >  1:485604/1‑140 (MQ=255)                                                      gcagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAcc                                                      >  1:601167/1‑140 (MQ=255)                                                        agcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCt                                                                    >  2:652669/1‑124 (MQ=255)                                                        agcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCt                                                                    <  1:652669/124‑1 (MQ=255)                                                        agcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAg                                                    >  2:219449/1‑140 (MQ=255)                                                        agcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAg                                                    >  1:783730/1‑140 (MQ=255)                                                          caACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGcc                                                  <  1:352843/140‑1 (MQ=255)                                                           aaCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAc                                                       >  1:415467/1‑134 (MQ=255)                                                           aaCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAc                                                       <  2:415467/134‑1 (MQ=255)                                                            aCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGccca                                                <  1:376402/140‑2 (MQ=255)                                                            aCAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCg                                                <  1:94129/140‑1 (MQ=255)                                                                    agcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGgcagca                                        >  1:267160/1‑140 (MQ=255)                                                                      cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                      <  1:700557/140‑1 (MQ=255)                                                                      cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                      <  1:326822/140‑1 (MQ=255)                                                                      cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                      <  2:695464/140‑1 (MQ=255)                                                                       gcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCacac                                     >  1:363190/1‑140 (MQ=255)                                                                             acaATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTg                               >  1:140608/1‑140 (MQ=255)                                                                                         gtgtCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAg                   >  1:647182/1‑140 (MQ=255)                                                                                           gtCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCa                 >  1:384358/1‑140 (MQ=255)                                                                                                   tGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcg         <  2:485604/140‑1 (MQ=255)                                                                                                   tGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcg         <  2:601167/140‑1 (MQ=255)                                                                                                        cTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGc    <  2:267160/140‑1 (MQ=255)                                                                                                        cTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGc    <  2:384358/140‑1 (MQ=255)                                                                                                          aTGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAg  <  1:47627/140‑1 (MQ=255)                                                                                                                     |                                                                                                                                 CACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAG  >  NC_000913/73559‑73803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑