Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	343,422	T→G	V180G (GTG→GGG)	yahK →	aldehyde reductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	343,422	0	T	G	100.0%	102.2 / NA	30	V180G (GTG→GGG)	yahK	aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/21);  total (9/21)

CGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTC  >  NC_000913/343285‑343557                                                                                                                                          |                                                                                                                                        cgtTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCgggg                                                                                                                                       >  2:528850/1‑140 (MQ=255) cgtTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCgggg                                                                                                                                       >  1:334719/1‑140 (MQ=255)   ttCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTc                                                                                                                                     >  1:188001/1‑140 (MQ=255)           cGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATcgg                                                                                                                             >  2:295223/1‑140 (MQ=255)           cGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATcgg                                                                                                                             <  2:258339/140‑1 (MQ=255)              tatGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATcggcgg                                                                                                                          >  1:393437/1‑140 (MQ=255)               atGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGt                                                                                                                         <  2:505310/140‑1 (MQ=255)                tGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTc                                                                                                                        <  2:283399/140‑1 (MQ=255)                        gTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATcggcg                                                                                                                           <  2:611861/129‑1 (MQ=255)                        gTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATcggcg                                                                                                                           >  1:611861/1‑129 (MQ=255)                          aTTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACatat                                                                                                              <  2:787096/140‑1 (MQ=255)                          aTTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGATAAAAAAGTGGGCGGGGTCGGCATCGGCGGGCTGGGGCaaat                                                                                                              >  2:649742/1‑140 (MQ=255)                            tCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATgg                                                                                                            >  1:371908/1‑140 (MQ=255)                            tCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGGCTGGGGCatattg                                                                                                            >  2:668559/1‑138 (MQ=255)                               tCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                         <  1:371026/140‑1 (MQ=255)                               tCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                         <  1:295223/140‑1 (MQ=255)                               tCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGATAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGa                                                                                                         <  1:649742/140‑1 (MQ=255)                                cACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGACAGGCCGGGCCGGGTAAATAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGAt                                                                                                        <  2:739704/140‑1 (MQ=255)                                      cAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGGCAAATTGGGGTTAAGCt                                                                                                  >  2:696606/1‑140 (MQ=255)                                                 ggcggcGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGa                                                                                       <  2:334719/140‑1 (MQ=255)                                                  gcggcgGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCAGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGAt                                                                                      <  1:305201/140‑1 (MQ=255)                                                   cggcggTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATg                                                                                     <  1:528850/140‑1 (MQ=255)                                                     gcggTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATggg                                                                                   <  2:783055/140‑1 (MQ=255)                                                          ggCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGcac                                                                              <  2:188001/140‑1 (MQ=255)                                                                     tgtgtgCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCa                                                                   <  1:668559/140‑1 (MQ=255)                                                                          gCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTAc                                                              <  2:393437/140‑1 (MQ=255)                                                                                       cGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCa                                                 <  1:493800/140‑1 (MQ=255)                                                                                                 gcTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAg                                       <  2:371908/140‑1 (MQ=255)                                                                                                      gTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCa                                  <  1:696606/140‑1 (MQ=255)                                                                                                       tCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCaa                                 <  1:91089/140‑1 (MQ=255)                                                                                                               aGGCCGGGCCGGGTAAAAAAGTGGGCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCGCGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTg                         <  1:157780/140‑1 (MQ=255)                                                                                                                                      ggCGGGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTc  >  1:43348/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGTTCATGAGCGATATGTTCTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGGATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTGGTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACATGTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGATGAAGTTGTTAACTC  >  NC_000913/343285‑343557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑