Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,619	T→C	R132R (CGT→CGC)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,619	0	T	C	100.0%	181.2 / NA	56	R132R (CGT→CGC)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/30);  total (26/30)

TGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATC  >  NC_000913/3245483‑3245751                                                                                                                                         |                                                                                                                                     tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTcc                                                                                                                                   >  2:258036/1‑140 (MQ=255)  gtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  <  1:636461/140‑1 (MQ=255)     cTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCg                                                                                                                               <  1:153188/140‑1 (MQ=255)      tgtgGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGc                                                                                                                              >  1:632445/1‑140 (MQ=255)        tggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAg                                                                                                                            <  1:829957/140‑1 (MQ=255)            gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAgg                                                                                                                        <  2:138938/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  <  1:186893/129‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       >  2:443827/1‑140 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGc                                                                                                                       <  1:814613/140‑1 (MQ=255)             cAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGGCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCa                                                                                                                                  >  2:186893/1‑129 (MQ=255)                ccGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTAc                                                                                                                    >  1:104996/1‑140 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAgg                                                                                                                        >  2:292008/1‑134 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAgg                                                                                                                        <  1:292008/134‑1 (MQ=255)                  gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACtt                                                                                                                  >  1:124068/1‑140 (MQ=255)                   cgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACttt                                                                                                                 <  2:664886/140‑1 (MQ=255)                        gCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGt                                                                                                            <  1:105567/140‑1 (MQ=255)                        gCAGGTAGCTGGGGTGGCCTGGCTGTTGCTAGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGt                                                                                                            <  1:182521/140‑1 (MQ=255)                         cAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTa                                                                                                           <  1:707036/140‑1 (MQ=255)                          aGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAg                                                                                                          <  2:104996/140‑1 (MQ=255)                           ggTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAgg                                                                                                         <  2:463493/140‑1 (MQ=255)                                  ggggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCg                                                                                                  >  1:631224/1‑140 (MQ=255)                                    ggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAt                                                                                                <  2:552500/140‑1 (MQ=255)                                      tGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGGATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTg                                                                                              >  2:376094/1‑140 (MQ=255)                                      tGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTg                                                                                              >  1:46894/1‑140 (MQ=255)                                      tGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTg                                                                                              >  1:536042/1‑140 (MQ=255)                                           tGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCg                                                                                         <  2:536042/140‑1 (MQ=255)                                             gCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGCAGCAGCGCTGATCCCGGCGGACATGATACCCCGATCCGCATGGGTCCCGGCGGAAGAGCGCTCCATCTCAGCAAGGTACTTTAACGGAGGTTCTTCGATTGGTGCgag                                                                                       >  2:281067/1‑139 (MQ=255)                                             gCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCgat                                                                                       >  2:199421/1‑140 (MQ=255)                                                   gCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgc                                                                                 >  1:456853/1‑140 (MQ=255)                                                       gtggtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccg                                                                             >  1:588978/1‑140 (MQ=255)                                                          gtgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccg                                                                          <  2:157260/140‑1 (MQ=255)                                                           tgCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccgc                                                                         <  1:632672/140‑1 (MQ=255)                                                            gCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgccact                                                                        <  2:631224/140‑4 (MQ=255)                                                             cggtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGCGCGATGATTGCGCCGCCGCtg                                                                       <  2:588978/140‑1 (MQ=255)                                                               gtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgcg                                                                                <  1:385481/129‑1 (MQ=255)                                                               gtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgcg                                                                                >  2:385481/1‑129 (MQ=255)                                                               gtcggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtggg                                                                     >  1:735976/1‑139 (MQ=255)                                                                  ggtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTggtggt                                                                  >  2:648424/1‑140 (MQ=255)                                                                   gtGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTa                                                                 >  2:322721/1‑140 (MQ=255)                                                                        gcgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGc                                                            <  2:334479/140‑1 (MQ=255)                                                                         cgGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCa                                                           >  1:25473/1‑140 (MQ=255)                                                                            aaGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGTGATGATTGCGCCGCCGCTGGTGGTATGGGCAATc                                                        >  2:50579/1‑140 (MQ=255)                                                                              gcagcGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGt                                                      >  1:561380/1‑140 (MQ=255)                                                                                    atgatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGca                                                <  2:46894/140‑1 (MQ=255)                                                                                      gatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATg                                                               <  1:527247/123‑1 (MQ=255)                                                                                      gatCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATg                                                               >  2:527247/1‑123 (MQ=255)                                                                                             gcggGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCa                                       >  2:583721/1‑140 (MQ=255)                                                                                                ggTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGAt                                    <  2:1362/140‑1 (MQ=255)                                                                                                 gTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATg                                   >  2:542405/1‑140 (MQ=255)                                                                                                  tCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATgg                                  >  2:438785/1‑140 (MQ=255)                                                                                                            aGCTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTatcat                        <  1:199421/140‑1 (MQ=255)                                                                                                              cTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCAtct                      >  1:117008/1‑140 (MQ=255)                                                                                                              cTCCGAATGGTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCAATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGAAATGCACAGCTGGCAGATGGCATTTATCAtct                      >  1:431696/1‑140 (MQ=255)                                                                                                                       gTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             <  1:258036/140‑1 (MQ=255)                                                                                                                       gTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             <  1:443827/140‑1 (MQ=255)                                                                                                                       gTTCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAt             <  1:655973/140‑1 (MQ=255)                                                                                                                        ttCCCGGCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCAtt            <  1:648424/140‑1 (MQ=255)                                                                                                                             ggCGAAAGAGCGCTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCt       <  1:376094/140‑1 (MQ=255)                                                                                                                                  aaGAGCGCTCCATCGCAGTAGCCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATc  <  2:208689/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGGTATGGGCAATCGTAATGCACAGCTGGCAGATGGCATTTATCATCTCCGGTGCATTGAGCTTTATC  >  NC_000913/3245483‑3245751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑