Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,976	A→G	intergenic (‑52/+57)	nlpI ← / ← pnp	lipoprotein NlpI/polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,976	0	A	G	100.0%	66.3 / NA	21	intergenic (‑52/+57)	nlpI/pnp	lipoprotein NlpI/polynucleotide phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/6);  total (15/6)

TTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAA‑G‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCT  >  NC_000913/3308841‑3309107                                                                                                                                        |                                                                                                                                      ttCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAg                                                                                                                                   >  2:421891/1‑140 (MQ=255)    aCTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCg                                                                                                                                <  1:776723/140‑1 (MQ=255)         tACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTg                                                                                                                           >  2:164621/1‑140 (MQ=255)          aCGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGc                                                                                                                          <  1:421891/140‑1 (MQ=255)                ggAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGtt                                                                                                                    >  1:818827/1‑140 (MQ=255)                   aGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTaaa                                                                                                                 <  1:540960/140‑1 (MQ=255)                               tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑G‑cccccccgcc                                                                                                   >  2:635318/1‑136 (MQ=255)                                  tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCa                                                                                                 >  1:777322/1‑140 (MQ=255)                                         ttAAGGGCTGGCGAAACAAAACCCCGGCGCAAAAAAGGCTTCTTTCCCCACTCCCGACGCCCACGGTAGAATGAACGGCCTTTTCCGGGTTGCGCGCATGGCGTCCTGCGCGGTAAAAG‑GCCCCCCCGCCGCAGCGGAgg                                                                                          <  2:777322/139‑1 (MQ=255)                                           aaGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTCCCCACCCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCGGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGc                                                                                        <  2:570416/140‑1 (MQ=255)                                                               cccAGCGAAAAAAAGGCTTCATTTCCCCCTCCCGAAGCCCACGGTTGAATGAACGTCCTGTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                    <  1:297470/139‑1 (MQ=255)                                                               acCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc                                                                    >  2:520699/1‑140 (MQ=255)                                                                    cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGtt                                                               >  1:21513/1‑140 (MQ=255)                                                                            aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTCCCCGGTTGCTAGCAGGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc                                                       <  2:439388/140‑1 (MQ=255)                                                                               cTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGa                                                    >  2:376193/1‑140 (MQ=255)                                                                                            tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgctg                                       >  1:72767/1‑140 (MQ=255)                                                                                               cgaaccccACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAGGGCGTCCTGCCCGGTTAAAC‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                    <  2:508586/134‑1 (MQ=255)                                                                                               caaaGCCCACGGTTAAAGAACCGTCCTTTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAACC‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                    <  2:21513/138‑1 (MQ=255)                                                                                               cGAAGCCCACGGTTGAATGACCGTCCTGTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                    <  2:156192/140‑1 (MQ=255)                                                                                                      cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                    <  2:298021/133‑1 (MQ=255)                                                                                                      cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag                                    >  1:298021/1‑133 (MQ=255)                                                                                                      cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTg                             >  1:167496/1‑140 (MQ=255)                                                                                                       aCGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                            <  1:376193/140‑1 (MQ=255)                                                                                                        cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgag                           >  1:123322/1‑140 (MQ=255)                                                                                                        cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgag                           >  1:286691/1‑140 (MQ=255)                                                                                                              aatgaaCGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt                     <  1:124281/140‑1 (MQ=255)                                                                                                                     gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGTTAAAAACC‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc              <  2:167496/140‑1 (MQ=255)                                                                                                                     gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc              >  2:288762/1‑140 (MQ=255)                                                                                                                     gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc              <  2:123322/140‑1 (MQ=255)                                                                                                                                 gTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt  >  2:279421/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAA‑G‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCT  >  NC_000913/3308841‑3309107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑