Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,391,146	T→C	T295A (ACC→GCC)	tldD ←	metalloprotease TldD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,391,146	0	T	C	100.0%	120.9 / NA	35	T295A (ACC→GCC)	tldD	metalloprotease TldD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

ACGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGTACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACC  >  NC_000913/3391009‑3391282                                                                                                                                          |                                                                                                                                         aCGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCATCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCac                                                                                                                                        >  2:804066/1‑140 (MQ=255)  cgcgcgTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGcaca                                                                                                                                       <  1:215545/140‑1 (MQ=255)  cgcgcgTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGcaca                                                                                                                                       <  2:434549/140‑1 (MQ=255)                     gCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGc                                                                                                                    >  1:156753/1‑140 (MQ=255)                     gCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGc                                                                                                                    >  2:565906/1‑140 (MQ=255)                           aGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCg                                                                                                              >  2:521904/1‑140 (MQ=255)                              cTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGAcc                                                                                                           <  2:141185/140‑1 (MQ=255)                               tttCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCt                                                                                                          <  2:755157/140‑1 (MQ=255)                                  cAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTc                                                                                                       >  1:795223/1‑140 (MQ=255)                                    gAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  1:222061/140‑1 (MQ=255)                                    gAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCATCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  1:804066/140‑1 (MQ=255)                                      aTGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACt                                                                                                   <  1:521904/140‑1 (MQ=255)                                          cGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     >  1:822808/1‑134 (MQ=255)                                          cGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCa                                                                                                     <  2:822808/134‑1 (MQ=255)                                              ctcAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCAATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTg                                                                                           <  1:212103/140‑1 (MQ=255)                                               tcAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGa                                                                                          >  1:783101/1‑140 (MQ=255)                                                  aTCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGt                                                                                       <  2:483185/140‑1 (MQ=255)                                                        aCGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCAcg                                                                                 <  1:152847/140‑1 (MQ=255)                                                                ctggcctggcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGa                                                                         <  2:51818/140‑1 (MQ=255)                                                                     ctggcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCg                                                                    <  1:97137/140‑1 (MQ=255)                                                                        gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  1:785249/1‑140 (MQ=255)                                                                        gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  2:153790/1‑140 (MQ=255)                                                                        gcGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCt                                                                 >  2:581308/1‑140 (MQ=255)                                                                          gTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTANNTACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               <  1:160064/140‑1 (MQ=255)                                                                             ccTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               >  2:130240/1‑137 (MQ=255)                                                                             ccTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTc                                                               <  1:130240/137‑1 (MQ=255)                                                                              cTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGa                                                           >  2:52049/1‑140 (MQ=255)                                                                              cTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGGAGCCTTCCAGa                                                           >  2:160064/1‑140 (MQ=255)                                                                                                ccGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCa                                         <  2:603168/140‑1 (MQ=255)                                                                                                ccGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCa                                         <  1:52049/140‑1 (MQ=255)                                                                                                   aaCCTCGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGc                                      >  1:802788/1‑140 (MQ=255)                                                                                                        cGGCGATCGACCATCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAg                                 <  1:153790/140‑1 (MQ=255)                                                                                                                     tCGTGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCCTCCCGACCGCGACCCACCCCCTCCTGCAACAGCACGCCCCGCCaa                    >  2:539636/1‑140 (MQ=255)                                                                                                                        tGCCATCATCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCt                 >  1:21302/1‑140 (MQ=255)                                                                                                                           catcatCAACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTgcg              <  1:565906/140‑1 (MQ=255)                                                                                                                             tcatcaACCACGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGcc            <  1:28668/140‑1 (MQ=255)                                                                                                                                       cGGCACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGGGACCAACCGCTTCATGCAACAGCACGCCCGGGCAACCTTCGCCAAGTACTAcc  >  2:143812/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         ACGCGCGTTGAGTTTATCCTGCATGTAGCCTTTCAGAATGCCGTTCTCAATCAGCACGTTGTACTGGCCTGGCGTACCTTCGTCATCAATCGCCACCGAACCTCGGCGATCGACCATCGTGCCATCATCAACCACGGTACACAGTTCTGAAGCCACCAGCTCCCCGACCTGTCCACTAAATACTGAAGTGCCACGGCGGTTGAAGTCGCCTTCCAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACC  >  NC_000913/3391009‑3391282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑