Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,481,747	C→G	G153G (GGC→GGG)	yheS →	ABC transporter ATP‑binding protein YheS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,481,747	0	C	G	100.0%	173.7 / NA	51	G153G (GGC→GGG)	yheS	ABC transporter ATP‑binding protein YheS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/25);  total (26/25)

TTGCGACCATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGCTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTATCAGGGCACG  >  NC_000913/3481617‑3481882                                                                                                                                   |                                                                                                                                         ttGCGACCATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATg                                                                                                                                 <  1:89706/140‑1 (MQ=255)      aCCATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCt                                                                                                                            <  2:639864/140‑1 (MQ=255)      aCCATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCt                                                                                                                            <  1:8857/140‑1 (MQ=255)        cATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTa                                                                                                                          >  2:593467/1‑140 (MQ=255)         aTTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTAt                                                                                                                                  <  2:277271/131‑1 (MQ=255)         aTTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTAt                                                                                                                                  >  1:277271/1‑131 (MQ=255)                    gCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAgg                                                                                                              >  1:380400/1‑140 (MQ=255)                    gCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAgg                                                                                                              <  2:434301/140‑1 (MQ=255)                         aTGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTg                                                                                                         >  1:32617/1‑140 (MQ=255)                           gCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGAt                                                                                                       >  1:302446/1‑140 (MQ=255)                                  aCGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAActgggca                                                                                                                  >  1:451164/1‑116 (MQ=255)                                  aCGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAActgggca                                                                                                                  <  2:451164/122‑7 (MQ=255)                                  aCGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGt                                                                                                >  1:39308/1‑140 (MQ=255)                                  aCGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGt                                                                                                >  2:652570/1‑140 (MQ=255)                                       tGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGa                                                                                           >  2:175799/1‑140 (MQ=255)                                       tGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGa                                                                                           >  2:567891/1‑140 (MQ=255)                                         gAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACt                                                                                         >  2:314121/1‑140 (MQ=255)                                            tATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTtgc                                                                                      >  2:469850/1‑140 (MQ=255)                                              ttCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTtgctg                                                                                    <  2:184928/140‑1 (MQ=255)                                                cGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTtgctgct                                                                                  >  1:758159/1‑140 (MQ=255)                                                         gctgcCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTcgacga                                                                         <  2:39308/140‑1 (MQ=255)                                                         gctgcCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTcgacga                                                                         <  1:567891/140‑1 (MQ=255)                                                            gcCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCAGTTCAGACTTGCTGCTGCTCGACGAAcc                                                                      >  1:794567/1‑140 (MQ=255)                                                             ccAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCg                                                                     >  2:9552/1‑140 (MQ=255)                                                                  ctgctgCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTaa                                                                <  2:302446/140‑1 (MQ=255)                                                                     ctgcACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAacca                                                             >  2:53896/1‑140 (MQ=255)                                                                       gcACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAaccacc                                                           >  1:750850/1‑140 (MQ=255)                                                                       gcACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAaccacc                                                           >  2:526096/1‑140 (MQ=255)                                                                          cGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTcgacga                                                                         >  1:253600/1‑123 (MQ=255)                                                                          cGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTcgacga                                                                         <  2:253600/123‑1 (MQ=255)                                                                           ggCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAaccacc                                                           >  2:423767/1‑136 (MQ=255)                                                                           ggCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAaccacc                                                           <  1:423767/136‑1 (MQ=255)                                                                             ccTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTAAGACTTGCTGCTGCTCGACGAACCGACTAACCACctcgatc                                                     >  2:253043/1‑140 (MQ=255)                                                                                 ggTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACctcgatctcg‑a                                                <  2:380400/140‑1 (MQ=255)                                                                                       aGCAATGAACAACTGGAGCGCCCGGTAAGTGGTTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGt                                          >  2:724838/1‑140 (MQ=255)                                                                                       aGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCGTATGCCg                                           >  1:428256/1‑140 (MQ=255)                                                                                         cAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTa                                        <  2:240658/140‑1 (MQ=255)                                                                                          aaTGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTAt                                       <  2:750850/140‑1 (MQ=255)                                                                                           aTGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATc                                      <  2:32617/140‑1 (MQ=255)                                                                                              aacaacTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATctgg                                   <  1:593467/140‑1 (MQ=255)                                                                                                   cTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGa                              >  2:443775/1‑140 (MQ=255)                                                                                                        gcgcCCGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAAt                         <  1:652570/140‑1 (MQ=255)                                                                                                           cccGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGc                      >  2:485493/1‑140 (MQ=255)                                                                                                           cccGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGc                      >  2:699792/1‑140 (MQ=255)                                                                                                            ccGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCt                     <  1:469850/140‑1 (MQ=255)                                                                                                            ccGGTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCt                     <  1:699792/140‑1 (MQ=255)                                                                                                              ggTAAGTGATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGa                   <  1:9552/140‑1 (MQ=255)                                                                                                                     gATTTTTCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTa            <  2:758159/140‑1 (MQ=255)                                                                                                                           tCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTATCAggg      <  1:443775/140‑1 (MQ=255)                                                                                                                           tCCGGGGGGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTAAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTATCAggg      <  1:253043/140‑1 (MQ=255)                                                                                                                               ggggGTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTATCAGGGCACg  <  1:485493/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TTGCGACCATTCATGGCAAGCTGGATGCTATTGACGCATGGAGTATTCGCTCCCGTGCTGCCAGCCTGCTGCACGGCCTCGGTTTCAGCAATGAACAACTGGAGCGCCCGGTAAGTGATTTTTCCGGGGGCTGGCGTATGCGTCTTAACCTTGCCCAGGCGCTGATTTGCCGTTCAGACTTGCTGCTGCTCGACGAACCGACTAACCACCTCGATCTCG‑ATGCCGTTATCTGGCTGGAAAAATGGCTGAAGAGCTATCAGGGCACG  >  NC_000913/3481617‑3481882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑