Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,548,433	T→C	T546T (ACA→ACG)	malQ ←	4‑alpha‑glucanotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,548,433	0	T	C	100.0%	229.6 / NA	67	T546T (ACA→ACG)	malQ	4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/30);  total (37/30)

CCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTA  >  NC_000913/3548304‑3548569                                                                                                                                  |                                                                                                                                         cccTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCaa                                                                                                                                <  1:56300/140‑1 (MQ=255) cccTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCaa                                                                                                                                >  1:209897/1‑140 (MQ=255)   cTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAAcc                                                                                                                              >  2:774376/1‑140 (MQ=255)   cTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAAc                                                                                                                               <  2:274682/139‑1 (MQ=255)   cTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAAc                                                                                                                               >  1:274682/1‑139 (MQ=255)           gCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGa                                                                                                                      >  1:250814/1‑140 (MQ=255)           gCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGa                                                                                                                      <  2:50689/140‑1 (MQ=255)            ccAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGAc                                                                                                                     <  1:101394/140‑1 (MQ=255)             cAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACt                                                                                                                    <  1:548344/140‑1 (MQ=255)             cAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACt                                                                                                                    <  1:285713/140‑1 (MQ=255)                ttCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCt                                                                                                                 >  1:553086/1‑140 (MQ=255)                ttCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCt                                                                                                                 >  1:255134/1‑140 (MQ=255)                ttCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCt                                                                                                                 <  2:209897/140‑1 (MQ=255)                             tACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCttt                                                                                                    <  2:250814/140‑1 (MQ=255)                                 gACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGt                                                                                                >  2:474713/1‑140 (MQ=255)                                 gACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGt                                                                                                >  2:17948/1‑140 (MQ=255)                                  aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                                                                               >  1:471240/1‑140 (MQ=255)                                  aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                                                                               >  1:165302/1‑140 (MQ=255)                                  aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                                                                               >  2:634700/1‑140 (MQ=255)                                  aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                                                                               >  2:676934/1‑140 (MQ=255)                                   ccGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGc                                                                                              >  2:364795/1‑140 (MQ=255)                                   ccGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGc                                                                                              >  2:203077/1‑140 (MQ=255)                                    cgcgCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCa                                                                                             <  2:255134/140‑1 (MQ=255)                                      cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCa                                                                                             >  2:333569/1‑138 (MQ=255)                                      cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCa                                                                                             <  1:333569/138‑1 (MQ=255)                                      cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                                                                           >  2:449434/1‑140 (MQ=255)                                      cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                                                                           >  2:787233/1‑140 (MQ=255)                                      cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                                                                           >  1:320820/1‑140 (MQ=255)                                             ccACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGtc                                                                                    <  1:17948/140‑1 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                                                                         <  1:191787/127‑1 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                                                                         >  2:191787/1‑127 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                                                                            >  2:413156/1‑140 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                                                                            >  2:291980/1‑140 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                                                                            >  1:298500/1‑140 (MQ=255)                                                     tCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                                                                            >  1:20211/1‑140 (MQ=255)                                                       cGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGt                                                                          <  2:165302/140‑1 (MQ=255)                                                        ggATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTc                                                                         <  1:203077/140‑1 (MQ=255)                                                        ggATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTc                                                                         <  1:364795/140‑1 (MQ=255)                                                          aTACAGCCCCAGGGTTTTGCCCAGCGTCAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGt                                                                       <  1:776746/140‑1 (MQ=255)                                                             cAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGtttt                                                                    >  2:220577/1‑140 (MQ=255)                                                                ccccAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGtct                                                                                   <  2:496549/122‑1 (MQ=255)                                                                ccccAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGtct                                                                                   >  1:496549/1‑122 (MQ=255)                                                                       gTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag                                                          <  1:634700/140‑1 (MQ=255)                                                                       gTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag                                                          <  2:298500/140‑1 (MQ=255)                                                                          ttGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCAc                                                       >  1:351913/1‑140 (MQ=255)                                                                              ccAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTg                                                   <  1:474713/140‑1 (MQ=255)                                                                                aGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTa                                                 <  1:787233/140‑1 (MQ=255)                                                                                  cGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag                                                          <  1:441761/129‑1 (MQ=255)                                                                                  cGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag                                                          >  2:441761/1‑129 (MQ=255)                                                                                  cGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAag                                               >  1:723765/1‑140 (MQ=255)                                                                                  cGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAag                                               >  2:757428/1‑140 (MQ=255)                                                                                  cGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAag                                               >  1:25110/1‑140 (MQ=255)                                                                                   gTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAaga                                              <  2:353251/140‑1 (MQ=255)                                                                                           cccGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTacacac                                      <  2:471240/140‑1 (MQ=255)                                                                                              gCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCgc                                   <  2:263466/140‑1 (MQ=255)                                                                                               cACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCgct                                  >  1:544410/1‑140 (MQ=255)                                                                                                    ccAGTAACCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCtgctgc                             >  2:448970/1‑140 (MQ=255)                                                                                                          aCCGCGCAGCGTTGGCAGGTCATGCGTCGCCGCAACCGACATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCt                       >  2:537471/1‑140 (MQ=255)                                                                                                              cgcAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGTCTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAcc                   >  2:707254/1‑140 (MQ=255)                                                                                                              cgcAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAcc                   >  1:690964/1‑140 (MQ=255)                                                                                                              cgcAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAcc                   >  1:302334/1‑140 (MQ=255)                                                                                                              cgcAGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTAcc                   >  1:512637/1‑140 (MQ=255)                                                                                                                 aGCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGAc                <  1:674235/140‑1 (MQ=255)                                                                                                                  gCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACa               <  1:715148/140‑1 (MQ=255)                                                                                                                  gCGTTGGCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACa               >  2:715148/1‑140 (MQ=255)                                                                                                                  gCGTTGGCAGGTCATCCGTCGCCGCATCCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGGCTTCTCGTGGTCGTTTTCGAAATAGAGCGCTTTGTAAGAGTACCCACCGCTGCTGCGCACCTTACCGACa               <  2:553086/140‑1 (MQ=255)                                                                                                                        gCAGGTCATGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATctct         <  2:690964/140‑1 (MQ=255)                                                                                                                               aTGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACcggta  <  1:757428/140‑1 (MQ=255)                                                                                                                               aTGCGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACcggta  <  2:302334/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         CCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCACTTTGTAAGAGTACACACCGCTGCTGCGCAGCTTACCGACAATCTCTACCGGTA  >  NC_000913/3548304‑3548569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑