Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,611,596	A→G	D46D (GAT→GAC)	yhhS ←	putative transporter YhhS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,611,596	0	A	G	100.0%	167.9 / NA	48	D46D (GAT→GAC)	yhhS	putative transporter YhhS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/23);  total (25/23)

CGCATAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGC  >  NC_000913/3611460‑3611731                                                                                                                                         |                                                                                                                                        cGCATAAACCGAAGACGACGATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCcatcacgtca                                                                                                                                      <  2:172753/140‑1 (MQ=255)    aTAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATgg                                                                                                                                   >  1:115143/1‑140 (MQ=255)       aaCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  2:115143/140‑1 (MQ=255)       aaCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  1:493292/140‑1 (MQ=255)        aCCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACAt                                                                                                                               <  2:553505/140‑1 (MQ=255)           gAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGc                                                                                                                            <  1:615443/140‑1 (MQ=255)            aaGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGcc                                                                                                                           <  1:763812/140‑1 (MQ=255)             aGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGccc                                                                                                                          >  2:659935/1‑140 (MQ=255)                  acAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                >  1:372301/1‑129 (MQ=255)                  acAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACa                                                                                                                                <  2:372301/129‑1 (MQ=255)                      tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGTATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             <  2:216797/128‑1 (MQ=255)                      tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             <  2:703919/128‑1 (MQ=255)                      tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             >  1:216797/1‑128 (MQ=255)                      tCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAg                                                                                                                             >  1:703919/1‑128 (MQ=255)                              ggTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGTGAGCgg                                                                                                         <  2:496601/140‑1 (MQ=255)                                 cccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCaa                                                                                                      >  1:4296/1‑140 (MQ=255)                                  ccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAc                                                                                                     >  2:818991/1‑140 (MQ=255)                                  ccAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAc                                                                                                     >  1:540589/1‑140 (MQ=255)                                   cAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAcc                                                                                                    <  1:666825/140‑1 (MQ=255)                                    aGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAAccc                                                                                                   >  2:534917/1‑140 (MQ=255)                                        aaTCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATg                                                                                               <  2:540589/140‑1 (MQ=255)                                                        ggCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGa                                                                               >  1:148787/1‑140 (MQ=255)                                                               gggCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                                                        >  2:822641/1‑140 (MQ=255)                                                               gggCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTaaa                                                                        <  1:330069/140‑1 (MQ=255)                                                                    gcTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGa                                                                   >  1:502636/1‑140 (MQ=255)                                                                        aGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACtat                                                               <  2:4296/140‑1 (MQ=255)                                                                             ggTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                                                     <  1:697682/129‑1 (MQ=255)                                                                             ggTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACAt                                                                     >  2:697682/1‑129 (MQ=255)                                                                                         ttGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAAt                                              >  2:160762/1‑140 (MQ=255)                                                                                               gCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgc                                        >  1:81028/1‑140 (MQ=255)                                                                                                 tGATAACCAGTCCTGCCCCGAATCCGCTACAGCCGATCACGACAAGGACATAGCCCGCTAATACAGCGAGCGGCAACACGATGGCGAGGCAGCTGGCGAAGTTAAACATGACTATAGAGACAAAGCGCAAATTCAGacgc                                      >  2:351995/1‑140 (MQ=255)                                                                                                 tGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGgcgc                                      <  1:822641/140‑1 (MQ=255)                                                                                                   aTAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCaa                                    >  1:192807/1‑140 (MQ=255)                                                                                                    tAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAt                                   <  2:677372/140‑1 (MQ=255)                                                                                                    tAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAAt                                   <  2:502636/140‑1 (MQ=255)                                                                                                         aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGtt                              >  2:122343/1‑140 (MQ=255)                                                                                                         aaTCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGtt                              >  1:739624/1‑140 (MQ=255)                                                                                                           tCCTGCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTa                            >  2:677577/1‑140 (MQ=255)                                                                                                               gCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAgcgc                        <  1:122343/140‑1 (MQ=255)                                                                                                               gCCCAGAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAgcgc                        >  1:322453/1‑140 (MQ=255)                                                                                                                    gAAGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGtt                   <  2:369777/140‑1 (MQ=255)                                                                                                                     aaGGCGCTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC‑AATTCAGGCGCAATCCGTTTAGCGCGGGTTCg                 >  1:646414/1‑140 (MQ=255)                                                                                                                          gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGt                    >  2:545177/1‑133 (MQ=255)                                                                                                                          gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGt                    <  1:545177/133‑1 (MQ=255)                                                                                                                          gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTa             >  2:455317/1‑140 (MQ=255)                                                                                                                          gcTAAAGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTa             <  2:322453/140‑1 (MQ=255)                                                                                                                               aGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGt        >  2:424577/1‑140 (MQ=255)                                                                                                                               aGCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGt        >  2:110997/1‑140 (MQ=255)                                                                                                                                gCCCATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGtt       <  1:677577/140‑1 (MQ=255)                                                                                                                                  ccATCACGTCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGC‑AATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCgg    <  2:646414/140‑1 (MQ=255)                                                                                                                                     tcacgtcaTGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGc  <  1:455317/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGCATAAACCGAAGACGACAATCTTTTTGGGTCCCAGCGAATCGGCGTAACGTCCGGCATGAGGGCGGCTCAGCAAGGTGGCGAAATATTGCAGGCTGATAACCAATCCTGCCCAGAAGGCGCTAAAGCCCATCACATCATGGACATAGCCCGGTAATACAGCGAGCGGCAACCCGATGGTGAGGTAGCTGGCGAAGTTAAACATGACTATAGAGACAATGCGCAAATTCAGGCGCAATCCGTTTAGCGCGGGTTCGGCTACGGGTTCGGGC  >  NC_000913/3611460‑3611731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑