Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,635,553	T→C	V238A (GTA→GCA)	yhiM →	inner membrane protein with a role in acid resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,635,553	0	T	C	100.0%	295.8 / NA	84	V238A (GTA→GCA)	yhiM	inner membrane protein with a role in acid resistance
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/47);  total (37/47)

TGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGG  >  NC_000913/3635418‑3635683                                                                                                                                        |                                                                                                                                   tGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTg                                                                                                                                <  1:738354/140‑1 (MQ=255)  gCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGc                                                                                                                               <  2:54348/140‑1 (MQ=255)   cTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCa                                                                                                                              <  1:682295/140‑1 (MQ=255)   cTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCa                                                                                                                              >  1:330234/1‑140 (MQ=255)   cTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCa                                                                                                                              <  2:254264/140‑1 (MQ=255)       ggTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCa                                                                                                                              <  1:174138/136‑1 (MQ=255)       ggTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCa                                                                                                                              >  2:174138/1‑136 (MQ=255)        gTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGa                                                                                                                         >  2:511987/1‑140 (MQ=255)          tAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGata                                                                                                                       >  1:383562/1‑140 (MQ=255)           aaCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAgg                                                                                                                            >  1:795108/1‑134 (MQ=255)           aaCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAgg                                                                                                                            <  2:795108/134‑1 (MQ=255)           aaCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGatat                                                                                                                      <  1:511987/140‑1 (MQ=255)           aaCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGatat                                                                                                                      <  2:43932/140‑1 (MQ=255)           aaCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGatat                                                                                                                      <  2:753997/140‑1 (MQ=255)            aCCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGAtata                                                                                                                     >  1:125299/1‑140 (MQ=255)             ccGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATAc                                                                                                                    >  1:643262/1‑140 (MQ=255)             ccGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATAc                                                                                                                    >  2:436717/1‑140 (MQ=255)                 aaTCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCt                                                                                                                >  2:824791/1‑140 (MQ=255)                       tgCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGt                                                                                                            >  1:394981/1‑138 (MQ=255)                       tgCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGt                                                                                                            <  2:394981/138‑1 (MQ=255)                       tgCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCa                                                                                                          >  1:444179/1‑140 (MQ=255)                        gCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTc                                                                                                                 <  2:338760/132‑1 (MQ=255)                        gCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTc                                                                                                                 >  1:338760/1‑132 (MQ=255)                         ccTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGt                                                                                                        <  2:383562/140‑1 (MQ=255)                         ccTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGt                                                                                                        <  2:684548/140‑1 (MQ=255)                         ccTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGt                                                                                                        <  1:62260/140‑1 (MQ=255)                             tCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCg                                                                                                    >  2:378053/1‑140 (MQ=255)                             tCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCg                                                                                                    >  2:760466/1‑140 (MQ=255)                             tCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCg                                                                                                    >  1:23288/1‑140 (MQ=255)                                 aTTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCTGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGc                                                                                                >  1:523985/1‑140 (MQ=255)                                 aTTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGc                                                                                                <  1:460859/140‑1 (MQ=255)                                       cTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAgcgc                                                                                          >  2:749254/1‑140 (MQ=255)                                       cTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAgcgc                                                                                          >  1:454431/1‑140 (MQ=255)                                       cTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAgcgc                                                                                          <  1:500396/140‑1 (MQ=255)                                             gCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACt                                                                                    <  1:378053/140‑1 (MQ=255)                                               cacaATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTgg                                                                                  >  1:502262/1‑140 (MQ=255)                                                     tGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCg                                                                            >  2:484567/1‑140 (MQ=255)                                                      gTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCgg                                                                           <  2:454431/140‑1 (MQ=255)                                                        ccATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         <  2:643262/140‑1 (MQ=255)                                                        ccATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         <  1:760466/140‑1 (MQ=255)                                                            cAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCattat                                                                     >  1:285877/1‑140 (MQ=255)                                                            cAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         >  2:543291/1‑136 (MQ=255)                                                            cAAACACGTAATACGTTTTCAACTAAAGAACACTAGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         <  1:543291/136‑1 (MQ=255)                                                              aaCACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTc                                                                   >  1:419378/1‑140 (MQ=255)                                                                 acGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTa                                                                >  1:626013/1‑140 (MQ=255)                                                                   gTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         <  2:289631/129‑1 (MQ=255)                                                                   gTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCa                                                                         >  1:289631/1‑129 (MQ=255)                                                                   gTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTAtt                                                              <  2:330234/140‑1 (MQ=255)                                                                      aTACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGc                                                           >  2:469705/1‑140 (MQ=255)                                                                       tACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGcc                                                          <  2:23288/140‑1 (MQ=255)                                                                       tACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGcc                                                          <  1:50315/140‑1 (MQ=255)                                                                       tACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGcc                                                          <  2:331995/140‑1 (MQ=255)                                                                        aCGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCt                                                         <  2:502262/140‑1 (MQ=255)                                                                           ttttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCgg                                                      <  1:436717/140‑1 (MQ=255)                                                                           ttttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCgg                                                      >  2:531664/1‑140 (MQ=255)                                                                            tttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGa                                                     <  2:824387/140‑1 (MQ=255)                                                                             ttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGaa                                                    <  2:315724/140‑1 (MQ=255)                                                                             ttCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGaa                                                    <  2:351420/140‑1 (MQ=255)                                                                              tCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAAt                                                   >  2:672094/1‑140 (MQ=255)                                                                               cAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATg                                                  <  1:43245/140‑1 (MQ=255)                                                                               cAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATg                                                  <  1:637224/140‑1 (MQ=255)                                                                                 aCTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGAt                                                <  1:665405/140‑1 (MQ=255)                                                                                 aCTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGAt                                                <  2:444179/140‑1 (MQ=255)                                                                                 aCTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGAt                                                <  1:758753/140‑1 (MQ=255)                                                                                       gAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTa                                          <  2:419378/140‑1 (MQ=255)                                                                                           acTGGCTGTGGTGTTATTGGGTTATTTTTCTCTGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGc                                      <  2:523985/140‑1 (MQ=255)                                                                                           acTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGc                                      <  2:125299/140‑1 (MQ=255)                                                                                           acTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGc                                      <  1:749254/140‑1 (MQ=255)                                                                                             tGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGAt                                                >  2:271418/1‑128 (MQ=255)                                                                                             tGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGAt                                                <  1:271418/128‑1 (MQ=255)                                                                                                  gtggtgTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATtct                               >  2:818659/1‑140 (MQ=255)                                                                                                    ggtgTTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCa                             >  1:745883/1‑140 (MQ=255)                                                                                                     gtgtTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGc                                      <  2:431166/130‑1 (MQ=255)                                                                                                     gtgtTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGc                                      >  1:431166/1‑130 (MQ=255)                                                                                                       gtTATTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCaaaa                          >  2:586874/1‑140 (MQ=255)                                                                                                          aTTGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTc                       >  2:264336/1‑140 (MQ=255)                                                                                                            tGGGTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTg                     <  1:264336/140‑1 (MQ=255)                                                                                                             gggTTATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTgg                    >  1:315408/1‑140 (MQ=255)                                                                                                                ttATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTa                 >  1:13462/1‑140 (MQ=255)                                                                                                                ttATTTTTCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTAATTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTa                 >  2:171891/1‑140 (MQ=255)                                                                                                                     tttCTCGGCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGc            <  1:504177/140‑1 (MQ=255)                                                                                                                            gCTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTa     <  1:192698/140‑1 (MQ=255)                                                                                                                             cTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTAt    <  2:315408/140‑1 (MQ=255)                                                                                                                             cTCAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTAt    >  1:47137/1‑140 (MQ=255)                                                                                                                               cAATCACGGCACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATgg  <  1:818659/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TGCTTGGGTTAACCGCAATCTGTGCCTGTCTAATTGGCCTTGTTGCCACAATTGTCCATCAAACACGTAATACGTTTTCAACTAAAGAACACTGGCTGTGGTGTTATTGGGTTATTTTTCTCGGCTCAATCACGGTACTGCAGGGGATATACGTCTTAGTCAGTTCCGATGCAAGCGCCCGACTGGCTCCCGGCATTATTCTTATTTGCCTCGGAATGATCTGTTACAGCATATTCTCAAAAGTCTGGCTACTGGCACTGGTATGG  >  NC_000913/3635418‑3635683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑