Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,713,867 A→G A72A (GCA→GCG)  yiaC → putative acyltransferase with acyl‑CoA N‑acyltransferase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,713,8670AG96.5% 183.8 / ‑2.1 57A72A (GCA→GCG) yiaCputative acyltransferase with acyl‑CoA N‑acyltransferase domain
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (26/29);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01

GCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCAC  >  NC_000913/3713750‑3714003
                                                                                                                     |                                                                                                                                        
gCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGAGATGTTTGTCGCACCGAAgg                                                                                                                    <  1:801974/140‑1 (MQ=255)
  gTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGcc                                                                                                                  >  1:196003/1‑140 (MQ=255)
     aCTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTc                                                                                                               >  1:726078/1‑140 (MQ=255)
         cATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAACACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgc                                                                                                           <  2:726078/140‑1 (MQ=255)
          aTTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  2:748273/140‑1 (MQ=255)
          aTTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  2:198365/140‑1 (MQ=255)
          aTTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  1:266009/140‑1 (MQ=255)
          aTTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  1:649709/140‑1 (MQ=255)
          aTTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  2:213965/140‑1 (MQ=255)
           ttCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcgc                                                                                                         >  1:358786/1‑140 (MQ=255)
           ttCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcgc                                                                                                         >  2:395074/1‑140 (MQ=255)
           ttCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcgc                                                                                                         >  2:284639/1‑140 (MQ=255)
                cTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          >  2:551932/1‑134 (MQ=255)
                cTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGgcg                                                                                                          <  1:551932/134‑1 (MQ=255)
                 tGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTAtt                                                                                                   >  1:280211/1‑140 (MQ=255)
                   gTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTgg                                                                                                 >  2:652367/1‑140 (MQ=255)
                      cGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTaa                                                                                              >  2:665469/1‑140 (MQ=255)
                        ggATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAgg                                                                                            >  1:289756/1‑140 (MQ=255)
                          aTGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          >  2:628610/1‑140 (MQ=255)
                          aTGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          <  2:473230/140‑1 (MQ=255)
                              cTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGa                                                                                      <  2:358786/140‑1 (MQ=255)
                               tATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGAt                                                                                     <  1:665469/140‑1 (MQ=255)
                                  cTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCa                                                                                  <  1:211929/140‑1 (MQ=255)
                                   ttGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAg                                                                                 >  2:558151/1‑140 (MQ=255)
                                    tGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGt                                                                                <  2:586641/140‑1 (MQ=255)
                                     gCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTa                                                                               >  2:46116/1‑140 (MQ=255)
                                     gCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTa                                                                               >  2:741556/1‑140 (MQ=255)
                                       cAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          >  2:323097/1‑127 (MQ=255)
                                       cAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          <  1:323097/127‑1 (MQ=255)
                                       cAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTg                                                                                       >  1:788360/1‑130 (MQ=255)
                                       cAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTg                                                                                       <  2:788360/130‑1 (MQ=255)
                                           gcgcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          >  2:14320/1‑123 (MQ=255)
                                           gcgcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGgcg                                                                                          <  1:14320/123‑1 (MQ=255)
                                           gcgcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTg                                                                                       >  1:656162/1‑126 (MQ=255)
                                           gcgcAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTg                                                                                       <  2:656162/126‑1 (MQ=255)
                                               aaaaCTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGcagcag                                                                     >  1:425707/1‑140 (MQ=255)
                                                 aaCTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAgcg                                                                   <  1:558151/140‑1 (MQ=255)
                                                  aCTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAgcgc                                                                  <  1:314139/140‑1 (MQ=255)
                                                      ggTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATc                                                              <  1:692814/140‑1 (MQ=255)
                                                      ggTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATc                                                              >  2:692814/1‑140 (MQ=255)
                                                       gTCTGGGAAGAAGATGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCa                                                                >  1:537362/1‑137 (MQ=255)
                                                       gTCTGGGAAGAAGATGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCa                                                                <  2:537362/137‑1 (MQ=255)
                                                             gaagaaGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCt                                                       <  1:741556/140‑1 (MQ=255)
                                                             gaagaaGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCt                                                       <  1:395074/140‑1 (MQ=255)
                                                              aagaagACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTg                                                      <  1:628610/140‑1 (MQ=255)
                                                               agaagaCGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGGCATCCCCACCTGa                                                     <  2:280211/140‑1 (MQ=255)
                                                                        tAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAgg                                            >  1:768208/1‑140 (MQ=255)
                                                                                       tGTCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATc                             >  2:300571/1‑140 (MQ=255)
                                                                                         tCAGCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCaa                           >  2:497723/1‑140 (MQ=255)
                                                                                            gCATTATGGAAGGCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCg                        <  1:256091/140‑1 (MQ=255)
                                                                                                       ggCCGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAAttt             >  2:101067/1‑140 (MQ=255)
                                                                                                       ggCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAAttt             >  1:780830/1‑140 (MQ=255)
                                                                                                         ccGATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAAttttt           >  1:581847/1‑140 (MQ=255)
                                                                                                           gATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTAc         <  1:46116/140‑1 (MQ=255)
                                                                                                           gATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTAc         <  2:425707/140‑1 (MQ=255)
                                                                                                           gATTTCTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTAc         <  1:737935/140‑1 (MQ=255)
                                                                                                           gATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTAc         <  2:780830/140‑1 (MQ=255)
                                                                                                                cTGGCGGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGc    <  1:101067/140‑1 (MQ=255)
                                                                                                                  ggcggcGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGcac  <  1:165537/140‑1 (MQ=255)
                                                                                                                     |                                                                                                                                        
GCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCAC  >  NC_000913/3713750‑3714003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑