Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,347	T→C	T9A (ACC→GCC)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,347	0	T	C	100.0%	198.1 / NA	57	T9A (ACC→GCC)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/34);  total (23/34)

CGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGT  >  NC_000913/3729211‑3729482                                                                                                                                         |                                                                                                                                        cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGcc                                                                                                                                      <  1:138960/140‑1 (MQ=255) cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGcc                                                                                                                                      <  1:619139/140‑1 (MQ=255) cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGcc                                                                                                                                      <  2:559092/140‑1 (MQ=255)      aGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGTTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGAt                                                                                                                                 <  2:218576/140‑1 (MQ=255)      aGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGAt                                                                                                                                 <  2:544828/140‑1 (MQ=255)         tgcctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTa                                                                                                                              >  2:500540/1‑140 (MQ=255)         tgcctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTa                                                                                                                              <  1:351012/140‑1 (MQ=255)         tgcctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTa                                                                                                                              <  1:36885/140‑1 (MQ=255)           cctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATc                                                                                                                            <  2:367855/140‑1 (MQ=255)           cctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATc                                                                                                                            <  2:397931/140‑1 (MQ=255)            ctgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATcc                                                                                                                           <  1:268836/140‑1 (MQ=255)             tgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATccc                                                                                                                          <  2:140294/140‑1 (MQ=255)             tgccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATccc                                                                                                                          <  2:560776/140‑1 (MQ=255)               ccaccaCTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGa                                                                                                                        >  2:824046/1‑140 (MQ=255)                caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTa                                                                                                                              >  1:490012/1‑133 (MQ=255)                caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTa                                                                                                                              <  2:490012/133‑1 (MQ=255)                caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATccc                                                                                                                          >  1:77837/1‑137 (MQ=255)                caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATccc                                                                                                                          <  2:77837/137‑1 (MQ=255)                 accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGata                                                                                                                      <  1:201450/140‑1 (MQ=255)                 accacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGata                                                                                                                      <  2:77381/140‑1 (MQ=255)                  ccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGAt                                                                                                                                 >  1:644535/1‑128 (MQ=255)                  ccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGAt                                                                                                                                 <  2:644535/128‑1 (MQ=255)                     cTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACa                                                                                                                  >  1:588679/1‑140 (MQ=255)                       gTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACata                                                                                                                <  1:531307/140‑1 (MQ=255)                       gTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACata                                                                                                                <  1:618807/140‑1 (MQ=255)                        ttCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGa                                                                                                                        <  1:522530/131‑1 (MQ=255)                        ttCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGa                                                                                                                        >  2:522530/1‑131 (MQ=255)                        ttCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACGTTTACACCCGACGCGCAAAGATCTATCCCGATATACatat                                                                                                               <  2:676952/140‑1 (MQ=255)                             ggTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGata                                                                                                                      <  2:817245/128‑1 (MQ=255)                             ggTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGata                                                                                                                      >  1:817245/1‑128 (MQ=255)                                   gTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCt                                                                                                    >  2:3453/1‑140 (MQ=255)                                    ttCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCtt                                                                                                   <  1:3453/140‑1 (MQ=255)                                         aCCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaa                                                                                              >  1:82982/1‑140 (MQ=255)                                         aCCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaa                                                                                              >  1:739275/1‑140 (MQ=255)                                          ccAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCTCCAAGATCTATCCCGATATACATATCGATCGTTCCTTaaaaaa                                                                                             <  2:739275/140‑1 (MQ=255)                                              agTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGc                                                                                         >  1:99387/1‑140 (MQ=255)                                              agTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGc                                                                                         >  2:436954/1‑140 (MQ=255)                                                   aTGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGt                                                                                    >  2:484138/1‑140 (MQ=255)                                                       gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCg                                                                                <  1:500540/140‑1 (MQ=255)                                                       gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCg                                                                                <  1:579003/140‑1 (MQ=255)                                                        ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCg                                                                                <  2:668004/139‑1 (MQ=255)                                                        ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCg                                                                                >  1:668004/1‑139 (MQ=255)                                                        ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGc                                                                               >  2:222404/1‑140 (MQ=255)                                                            cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTAcc                                                                           >  1:219589/1‑140 (MQ=255)                                                                            tCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACg                                                           >  1:212150/1‑140 (MQ=255)                                                                             ccGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACgg                                                          >  2:47931/1‑140 (MQ=255)                                                                              cGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                         <  1:228010/140‑1 (MQ=255)                                                                                    cgcAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGcac                                                   <  2:99387/140‑1 (MQ=255)                                                                                                        gCTCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                               <  2:567380/140‑1 (MQ=255)                                                                                                          tCGTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAAc                             >  2:601070/1‑140 (MQ=255)                                                                                                            gTTGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                           >  2:71476/1‑140 (MQ=255)                                                                                                              tGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAt                                      >  1:83303/1‑127 (MQ=255)                                                                                                              tGAGCAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAt                                      <  2:83303/127‑1 (MQ=255)                                                                                                                  cAAAATAACTTTTACGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATg                     <  1:824046/140‑1 (MQ=255)                                                                                                                               aCGCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAttt        >  1:291704/1‑140 (MQ=255)                                                                                                                                 gCCCGAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACATATAATGGTTTACCAGATTTTc      <  2:588679/140‑1 (MQ=255)                                                                                                                                     gAGGCGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgt  <  1:198566/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGT  >  NC_000913/3729211‑3729482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑