Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	347,702	T→C	T248A (ACG→GCG)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	347,702	0	T	C	100.0%	63.6 / NA	22	T248A (ACG→GCG)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

CCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCAT  >  NC_000913/347570‑347836                                                                                                                                     |                                                                                                                                       ccAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTccccc                                                                                                                                 <  1:549685/140‑1 (MQ=255)              ggCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACAccg                                                                                                                    <  2:268440/140‑1 (MQ=255)              ggCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACAccg                                                                                                                    <  1:818077/140‑1 (MQ=255)                    cGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCAc                                                                                                              >  1:655082/1‑140 (MQ=255)                                 aCGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATa                                                                                                 <  2:137828/140‑1 (MQ=255)                                 aCGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATa                                                                                                 >  1:137828/1‑140 (MQ=255)                                                tGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTg                                                                                  >  1:182372/1‑140 (MQ=255)                                                                         cGTGGCGGGGAAAATATTTCCGATGAAACGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCCCTTTCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATACTCTTCCGCACTTGTTCCCTCTGTCGTGAt                                                         >  2:496105/1‑140 (MQ=255)                                                                         cGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCTCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGAt                                                         >  1:84645/1‑140 (MQ=255)                                                                           tGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGGATACAGCAAAATAGTCTTCCGTACTTGTTCCCTCTGTGGTGAttt                                                       >  2:669792/1‑139 (MQ=255)                                                                           tGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTg                                                       >  2:198856/1‑140 (MQ=255)                                                                              cGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGAcc                                                    <  2:655082/140‑1 (MQ=255)                                                                                                 gAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACg                                 >  1:821857/1‑140 (MQ=255)                                                                                                 gAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACg                                 >  2:539168/1‑140 (MQ=255)                                                                                                  aaTCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGt                                <  2:821857/140‑1 (MQ=255)                                                                                                  aaTCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGt                                <  2:66333/140‑1 (MQ=255)                                                                                                      gCCTGGGCCGCCGCCTCTTTGCCGGTCCCCGCTCCCCCCTCAATCACCGCCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACg                            <  2:468227/140‑1 (MQ=255)                                                                                                               gccAGCTCTTTGCCCGTCCCCGCTTCCCCCTCAATCAACACCTCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAg                   <  2:84645/140‑1 (MQ=255)                                                                                                                       tttGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGc           >  1:821181/1‑140 (MQ=255)                                                                                                                         tGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCgg         <  1:496105/140‑1 (MQ=255)                                                                                                                         tGCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCgg         <  1:533365/140‑1 (MQ=255)                                                                                                                          gCCCGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCggg        >  1:280128/1‑140 (MQ=255)                                                                                                                            ccGTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTg      <  1:731857/140‑1 (MQ=255)                                                                                                                              gTCCCCGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGc    <  2:506813/140‑1 (MQ=255)                                                                                                                                ccccGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGc    <  2:331505/138‑1 (MQ=255)                                                                                                                                ccccGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGc    >  1:331505/1‑138 (MQ=255)                                                                                                                                ccccGCTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCAt  <  1:198856/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCAT  >  NC_000913/347570‑347836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑