| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,774,056 | T→C | G592G (GGT→GGC) | mtlA → | mannitol‑specific PTS enzyme IICBA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,774,056 | 0 | T | C | 98.4% | 215.8 / ‑5.3 | 62 | G592G (GGT→GGC) | mtlA | mannitol‑specific PTS enzyme IICBA component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (30/31); minor base G (1/0); total (31/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAG > NC_000913/3773920‑3774191 | gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAATATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCAtt > 1:435750/1‑140 (MQ=255) aGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATtgct < 2:391598/140‑1 (MQ=255) tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAGAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATtgctgc > 2:255308/1‑140 (MQ=255) tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATtgctgc > 1:62409/1‑140 (MQ=255) tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATtgctgc > 1:261982/1‑140 (MQ=255) tCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGt > 2:364475/1‑140 (MQ=255) tGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTaacaac > 2:757526/1‑140 (MQ=255) cacaCGGTACGGTTGAAGCGAAAGATCGCGTACTGAGAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAg < 1:255308/140‑1 (MQ=255) cacaCGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAg < 2:228162/140‑1 (MQ=255) cacaCGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAg < 1:655426/140‑1 (MQ=255) cacaCGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAg < 1:232654/140‑1 (MQ=255) cacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGca < 2:31025/140‑1 (MQ=255) tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTc > 2:134122/1‑140 (MQ=255) tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTc > 1:146097/1‑140 (MQ=255) ttGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGtt > 2:140079/1‑140 (MQ=255) ttGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGtt > 2:543993/1‑140 (MQ=255) gAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt < 2:62409/140‑1 (MQ=255) aaGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATc > 2:274968/1‑140 (MQ=255) gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAc > 1:652998/1‑140 (MQ=255) aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTTCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc > 1:409736/1‑140 (MQ=255) aGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGt < 1:515621/129‑1 (MQ=255) aGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGt > 2:515621/1‑129 (MQ=255) gcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGAcc < 1:63292/140‑1 (MQ=255) cgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCa > 1:33711/1‑140 (MQ=255) cTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGc < 1:708623/140‑1 (MQ=255) gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCAc < 1:351778/140‑1 (MQ=255) gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCAc < 1:134122/140‑1 (MQ=255) aaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTgg > 1:121157/1‑140 (MQ=255) gCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat < 1:233197/140‑1 (MQ=255) cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACGTTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat > 1:476819/1‑139 (MQ=255) cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACGTTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat < 2:476819/139‑1 (MQ=255) cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGatgatg > 2:727032/1‑140 (MQ=255) cgtcgtGTTCTGCCAGTACCAGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGatgatg > 1:767840/1‑140 (MQ=255) cAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa < 1:727032/140‑1 (MQ=255) cAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa < 2:33711/140‑1 (MQ=255) gTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGc > 2:342628/1‑140 (MQ=255) tACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGGATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCg > 2:573247/1‑140 (MQ=255) aCCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGt > 1:424326/1‑140 (MQ=255) ggAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTgg < 1:364475/140‑1 (MQ=255) gAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGc > 2:665294/1‑140 (MQ=255) aaGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGca < 2:594579/140‑1 (MQ=255) tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGt < 1:416023/128‑1 (MQ=255) tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGt > 2:416023/1‑128 (MQ=255) tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcacc > 1:695120/1‑140 (MQ=255) tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcacc > 1:488255/1‑140 (MQ=255) cgcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcaccac < 2:767840/140‑1 (MQ=255) cTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACAccacca > 1:267472/1‑140 (MQ=255) ttCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg < 2:424326/140‑1 (MQ=255) ttCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg < 2:760260/140‑1 (MQ=255) aagaagaagATGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAt > 1:786926/1‑140 (MQ=255) gaTGACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCt > 2:299225/1‑140 (MQ=255) gACATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa < 2:695120/140‑1 (MQ=255) aCATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGaa < 2:121157/140‑1 (MQ=255) cATTGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAc < 2:267472/140‑1 (MQ=255) tGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg < 1:654942/125‑1 (MQ=255) tGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg > 2:654942/1‑125 (MQ=255) tGCCCGTCTGGTGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc > 1:205779/1‑140 (MQ=255) tGATTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgt < 1:140079/140‑1 (MQ=255) aTTGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaa < 2:205779/140‑1 (MQ=255) ttGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaag < 1:543993/140‑1 (MQ=255) ttGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaag < 1:299225/140‑1 (MQ=255) ttGGCATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaag < 1:665294/140‑1 (MQ=255) | GTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAG > NC_000913/3773920‑3774191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |