Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,687	T→A	I415F (ATC→TTC)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,687	0	T	A	100.0%	200.7 / NA	60	I415F (ATC→TTC)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/26);  total (34/26)

TTCTGCACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTG  >  NC_000913/3834551‑3834820                                                                                                                                         |                                                                                                                                      ttCTGCACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGc                                                                                                                                    <  2:644586/140‑1 (MQ=255)      cACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGaa                                                                                                                               <  1:588456/140‑1 (MQ=255)      cACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGaa                                                                                                                               <  1:730123/140‑1 (MQ=255)      cACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGaa                                                                                                                               <  2:312743/140‑1 (MQ=255)      cACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGATTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGaa                                                                                                                               >  2:730123/1‑140 (MQ=255)           cttGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATc                                                                                                                          >  1:329945/1‑140 (MQ=255)           cttGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATc                                                                                                                          >  1:342063/1‑140 (MQ=255)              gagaGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTg                                                                                                                       >  2:450585/1‑140 (MQ=255)                gagGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGaa                                                                                                                     <  2:699847/140‑1 (MQ=255)                gagGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGaa                                                                                                                     <  2:162009/140‑1 (MQ=255)                   gTGAATTGTTGCTGGCTACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAg                                                                                                                  <  2:744198/140‑1 (MQ=255)                   gTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAg                                                                                                                  <  1:583115/140‑1 (MQ=255)                   gTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAg                                                                                                                  >  1:146236/1‑140 (MQ=255)                    tGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAGCAATCGTGAATAgc                                                                                                                 >  2:697844/1‑140 (MQ=255)                      aaTTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATa                                                                                                                   <  2:693818/136‑1 (MQ=255)                      aaTTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATa                                                                                                                   >  1:693818/1‑136 (MQ=255)                           ttgCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCaataa                                                                                                          <  2:342063/140‑1 (MQ=255)                              cTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAatga                                                                                                       >  2:825479/1‑140 (MQ=255)                               tGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAatgat                                                                                                      <  1:450585/140‑1 (MQ=255)                                ggCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCaataa                                                                                                          <  1:295378/135‑1 (MQ=255)                                ggCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCaataa                                                                                                          >  2:295378/1‑135 (MQ=255)                                  cAACGGCGTTTACCCTAAGCCAGCTGTTCCATAACGGTTTTCAGATTGCGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAACGATGCt                                                                                                   <  2:181616/140‑1 (MQ=255)                                   aaCGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTa                                                                                                  >  2:723784/1‑140 (MQ=255)                                        cGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGt                                                                                             >  1:396904/1‑140 (MQ=255)                                            tACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGgcg                                                                                         <  1:723784/140‑1 (MQ=255)                                             aCCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGgcgc                                                                                        >  1:269950/1‑140 (MQ=255)                                                        gCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCt                                                                             >  2:453172/1‑140 (MQ=255)                                                        gCTGTTCCATAACGGTTTTCAGATTGTGCGTCGCGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCt                                                                             >  2:246532/1‑140 (MQ=255)                                                         cTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCtt                                                                            >  1:228109/1‑140 (MQ=255)                                                            ttCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCttttt                                                                         <  1:73154/140‑1 (MQ=255)                                                             tCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTc                                                                        >  1:709191/1‑140 (MQ=255)                                                                aTAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGc                                                                     <  1:581242/140‑1 (MQ=255)                                                                 tAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGcc                                                                    <  2:709191/140‑1 (MQ=255)                                                                   aCGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGc                                                                  >  2:617901/1‑140 (MQ=255)                                                                    cGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGca                                                                 <  2:162533/140‑1 (MQ=255)                                                                    cGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGca                                                                 >  1:484858/1‑140 (MQ=255)                                                                      gTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGcatc                                                               >  2:684332/1‑140 (MQ=255)                                                                      gTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGcatc                                                               >  2:425011/1‑140 (MQ=255)                                                                       ttttCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGcatca                                                              >  2:299373/1‑140 (MQ=255)                                                                         ttCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCata                                                            >  2:106255/1‑140 (MQ=255)                                                                           cAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATc                                                          <  2:676881/140‑1 (MQ=255)                                                                                 gtgCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCGGCCCCATCATATCCGCCAt                                                    <  2:396904/140‑1 (MQ=255)                                                                                    cgtcgtGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAg                                                 >  1:359249/1‑140 (MQ=255)                                                                                         tgagtgagTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGc                                            <  1:453172/140‑1 (MQ=255)                                                                                            gtgagtAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATc                                         <  2:242043/140‑1 (MQ=255)                                                                                            gtgagtAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATc                                         <  1:425011/140‑1 (MQ=255)                                                                                            gtgagtAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATc                                         <  2:484858/140‑1 (MQ=255)                                                                                             tgagtAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATcc                                        >  2:216343/1‑140 (MQ=255)                                                                                              gagtAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCa                                       <  1:684332/140‑1 (MQ=255)                                                                                                    tagCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGAt                                 >  2:456735/1‑140 (MQ=255)                                                                                                      gCGTTTAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATaa                               >  1:763843/1‑140 (MQ=255)                                                                                                         tttAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAagag                            >  2:233559/1‑140 (MQ=255)                                                                                                           tAGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAACATCCAGCCGATAAGAgcg                          >  1:796843/1‑140 (MQ=255)                                                                                                            aGCGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAgcgc                         >  2:420438/1‑140 (MQ=255)                                                                                                              cGATAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGcgccg                       >  2:653740/1‑140 (MQ=255)                                                                                                                 tAATCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGccgccg                    <  1:233559/140‑1 (MQ=255)                                                                                                                    tCGCGCTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAg                 >  2:403585/1‑140 (MQ=255)                                                                                                                      gcgcTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGc               <  2:329945/140‑1 (MQ=255)                                                                                                                      gcgcTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGc               <  1:223254/140‑1 (MQ=255)                                                                                                                       cgcTCAGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGcc              >  2:159203/1‑140 (MQ=255)                                                                                                                            aGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAAccc         >  1:551486/1‑140 (MQ=255)                                                                                                                            aGCAAATAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAAccc         >  2:413664/1‑140 (MQ=255)                                                                                                                                 aTAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGtt    >  1:684862/1‑140 (MQ=255)                                                                                                                                 aTAACAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGtt    <  1:106255/140‑1 (MQ=255)                                                                                                                                   aaCAGAACGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTg  <  1:420438/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TTCTGCACTTCTTGAGAGGTGAATTGTTGCTGGCAACGGCGTTTACCCTGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTG  >  NC_000913/3834551‑3834820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑