Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,500	A→G	T450A (ACC→GCC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,500	0	A	G	100.0%	143.8 / NA	42	T450A (ACC→GCC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/24);  total (18/24)

CCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGG  >  NC_000913/3992381‑3992635                                                                                                                        |                                                                                                                                        ccAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGGTTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGccgccg                                                                                                                     >  1:626811/1‑140 (MQ=255)    gTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCgccgggc                                                                                                                  <  1:11990/140‑1 (MQ=255)                 cGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACTCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGtt                                                                                                     <  1:807243/140‑1 (MQ=255)                  ggCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTc                                                                                                    <  2:46068/140‑1 (MQ=255)                  ggCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATCTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTc                                                                                                    <  1:725125/140‑1 (MQ=255)                      tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                                                <  2:412891/140‑1 (MQ=255)                      tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                                                >  2:658507/1‑140 (MQ=255)                      tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                                                >  2:367285/1‑140 (MQ=255)                      tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGt                                                                                                >  2:48767/1‑140 (MQ=255)                          gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGGTTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                            <  2:626811/140‑1 (MQ=255)                          gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                            <  2:33580/140‑1 (MQ=255)                          gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                            <  1:658507/140‑1 (MQ=255)                          gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                            <  1:519340/140‑1 (MQ=255)                          gACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGt                                                                                            <  1:404106/140‑1 (MQ=255)                                       tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                                               >  1:430656/1‑140 (MQ=255)                                       tATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcg                                                                               >  1:203880/1‑140 (MQ=255)                                        aTGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgc                                                                              <  1:358331/140‑1 (MQ=255)                                         tGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACcgcg                                                                             >  2:136396/1‑140 (MQ=255)                                              cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                                         <  1:238522/139‑1 (MQ=255)                                              cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGcc                                                                         >  2:238522/1‑139 (MQ=255)                                              cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCa                                                                        >  1:667976/1‑140 (MQ=255)                                              cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCa                                                                        >  2:239356/1‑140 (MQ=255)                                                 ttGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAat                                                                     >  2:534134/1‑140 (MQ=255)                                                     aGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTg                                                                 <  2:565997/140‑1 (MQ=255)                                                                 tAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGAtcatca                                                     >  1:685562/1‑140 (MQ=255)                                                                       gACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatc                                               >  1:73202/1‑140 (MQ=255)                                                                          gCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatcagc                                            <  2:816662/140‑1 (MQ=255)                                                                          gCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatcagc                                            >  2:178885/1‑140 (MQ=255)                                                                          gCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatcagc                                            >  1:763323/1‑140 (MQ=255)                                                                              gTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGatcatc                                                      <  1:554873/126‑1 (MQ=255)                                                                              gTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGatcatc                                                      >  2:554873/1‑126 (MQ=255)                                                                               tAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTg                                       <  2:710728/140‑1 (MQ=255)                                                                                    ccGCAGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAata                                  <  1:576620/140‑1 (MQ=255)                                                                                        aGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatcagc                                            <  2:392260/126‑1 (MQ=255)                                                                                        aGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATcatcagccatcagc                                            >  1:392260/1‑126 (MQ=255)                                                                                        aGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                         <  1:743386/129‑1 (MQ=255)                                                                                        aGATTTCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGc                                         >  2:743386/1‑129 (MQ=255)                                                                                             tCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTa                         <  2:73202/140‑1 (MQ=255)                                                                                             tCACCCGATCTCTCGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTa                         <  1:136396/140‑1 (MQ=255)                                                                                                     tctctcGGAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATa                 <  1:534134/140‑1 (MQ=255)                                                                                                           ggAACCGAATCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACtgg           <  1:405743/140‑1 (MQ=255)                                                                                                                    tCTGGCCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTggg  <  2:203880/140‑1 (MQ=255)                                                                                                                        |                                                                                                                                        CCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCGCCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCGCGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGG  >  NC_000913/3992381‑3992635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑