Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,054,870	T→G	T137P (ACG→CCG)	glnG ←	DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,054,870	0	T	G	100.0%	197.8 / NA	56	T137P (ACG→CCG)	glnG	DNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/25);  total (31/25)

GGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCC  >  NC_000913/4054738‑4054990                                                                                                                                     |                                                                                                                         gggCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGTATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCg                                                                                                                   <  1:801550/140‑1 (MQ=255)   gCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGtt                                                                                                                 >  1:387505/1‑140 (MQ=255)       gAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGc                                                                                                             >  1:828933/1‑140 (MQ=255)            aCCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAAc                                                                                                        >  2:553591/1‑140 (MQ=255)                      tACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGgc                                                                                              <  2:828933/140‑1 (MQ=255)                      tACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGgc                                                                                              >  1:525702/1‑140 (MQ=255)                           gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTAc                                                                                                   >  2:450204/1‑130 (MQ=255)                           gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTAc                                                                                                   <  1:450204/130‑1 (MQ=255)                           gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGgctgctg                                                                                         <  1:174483/140‑1 (MQ=255)                           gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGgctgctg                                                                                         >  2:697105/1‑140 (MQ=255)                           gTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGgctgctg                                                                                         >  1:175950/1‑140 (MQ=255)                               cGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGt                                                                                     <  2:462506/140‑1 (MQ=255)                                 gATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTc                                                                                   <  1:474207/140‑1 (MQ=255)                                      gCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGt                                                                              >  2:819137/1‑140 (MQ=255)                                       ccGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTa                                                                             <  2:91563/140‑1 (MQ=255)                                         gTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAAt                                                                           <  2:98293/140‑1 (MQ=255)                                                  cACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                  >  1:51822/1‑140 (MQ=255)                                                  cACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                  <  2:525702/140‑1 (MQ=255)                                                  cACGCTAATAGAAGAACACGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAg                                                                  >  2:780440/1‑140 (MQ=255)                                                       tAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAgcgcgc                                                             >  1:348810/1‑140 (MQ=255)                                                       tAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAgcgcgc                                                             >  1:48818/1‑140 (MQ=255)                                                            gaagaaCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAAc                                                        <  1:553591/140‑1 (MQ=255)                                                                 aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                   >  1:88304/1‑140 (MQ=255)                                                                 aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                   >  2:485126/1‑140 (MQ=255)                                                                 aCGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAgcg                                                   >  1:168802/1‑140 (MQ=255)                                                                   gcgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGcc                                                 >  1:63118/1‑140 (MQ=255)                                                                   gcgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGcc                                                 >  2:459004/1‑140 (MQ=255)                                                                    cgAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCa                                                >  1:539927/1‑140 (MQ=255)                                                                     gAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCAc                                               <  2:348810/140‑1 (MQ=255)                                                                     gAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCAc                                               >  1:438827/1‑140 (MQ=255)                                                                         gCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCt                                           <  1:459004/140‑1 (MQ=255)                                                                             accgaTAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTtcgt                                       <  1:485126/140‑1 (MQ=255)                                                                                        ggAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAAc                            >  2:790737/1‑140 (MQ=255)                                                                                        ggAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAAc                            >  2:532372/1‑140 (MQ=255)                                                                                        ggAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAAc                            >  1:263073/1‑140 (MQ=255)                                                                                          aaCACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                              <  2:221822/136‑1 (MQ=255)                                                                                          aaCACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCaa                              >  1:221822/1‑136 (MQ=255)                                                                                           acacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGt                         <  2:263073/140‑1 (MQ=255)                                                                                            cacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                        >  2:305241/1‑140 (MQ=255)                                                                                            cacGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                        >  2:768081/1‑140 (MQ=255)                                                                                            cacGTCCTACATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGtt                        >  2:69147/1‑140 (MQ=255)                                                                                              cGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTg                      <  1:790737/140‑1 (MQ=255)                                                                                              cGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTg                      <  1:768081/140‑1 (MQ=255)                                                                                              cGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTg                      <  1:532372/140‑1 (MQ=255)                                                                                              cGTCCTACATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTg                      <  1:69147/140‑1 (MQ=255)                                                                                                 ccTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGc                   <  2:48818/140‑1 (MQ=255)                                                                                                    gCATGGCTGGCGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGa                >  2:154024/1‑140 (MQ=255)                                                                                                    gCATGGCTGGCACTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGa                >  1:771669/1‑140 (MQ=255)                                                                                                           tggcGCTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCaa         >  1:763763/1‑140 (MQ=255)                                                                                                           tggcGCTTCGCCGATAATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCaa         >  1:376494/1‑140 (MQ=255)                                                                                                               gcTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc     <  2:175950/140‑1 (MQ=255)                                                                                                               gcTTCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc     <  2:438827/140‑1 (MQ=255)                                                                                                               gcTTCGCCGATAATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc     <  2:376494/140‑1 (MQ=255)                                                                                                                  tCGCCGATGATATCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGcccc  <  1:305241/140‑1 (MQ=255)                                                                                                                             atCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc     >  1:747118/1‑126 (MQ=255)                                                                                                                             atCGGTCGGTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGc     <  2:747118/126‑1 (MQ=255)                                                                                                                                     |                                                                                                                         GGGCATGAGCGACCAGTTCTTTACCGGTGCCGGATTCGCCGTTAATCAGCACGCTAATAGAAGAACGCGAAAGCCGACCGATAATACGGAACACGTCCTGCATGGCTGGCGCTTCGCCGATGATATCGGTCGTTGGGCCGTTAAGCTGAACATTACGCGGCTGCTGCTGTTCCTGGTAATGACTGATAGCGCGCTCAACCAGCGCCACTGCTTCGTCGATATCAAACGGTTTGGGCAGATAATCAAACGCCCC  >  NC_000913/4054738‑4054990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑