Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,074,085	T→C	L116P (CTG→CCG)	yihV →	6‑deoxy‑6‑sulfofructose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,074,085	0	T	C	100.0%	115.4 / NA	34	L116P (CTG→CCG)	yihV	6‑deoxy‑6‑sulfofructose kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

GCTGGCAGAGCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTG  >  NC_000913/4073948‑4074215                                                                                                                                          |                                                                                                                                   gctgGCAGAGCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCtgccgc                                                                                                                                  >  1:156224/1‑140 (MQ=255)     gCAGAGCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGa                                                                                                                              >  2:333827/1‑140 (MQ=255)      cAGAGCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGAc                                                                                                                             >  1:696174/1‑140 (MQ=255)         agCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCa                                                                                                                          <  2:353840/140‑1 (MQ=255)           cTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCaga                                                                                                                        <  2:633263/140‑1 (MQ=255)            tGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCagag                                                                                                                       >  1:705085/1‑140 (MQ=255)                 tCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGtt                                                                                                                  <  2:745583/140‑1 (MQ=255)                           ttAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAAtt                                                                                                        >  2:711349/1‑140 (MQ=255)                                      tACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTGCCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCa                                                                                             <  1:282103/140‑1 (MQ=255)                                         acCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTTATTTCTCTCAGTg                                                                                          <  1:333827/140‑1 (MQ=255)                                         acCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTg                                                                                          <  2:685850/140‑1 (MQ=255)                                               cGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGAtgt                                                                                    >  2:556611/1‑140 (MQ=255)                                                            cGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGAt                                                                       >  2:560827/1‑140 (MQ=255)                                                            cGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCAACCCCGGCTCGGCCGCCTGCCCCGGGTTGCTTGGAGGAAATTGATTTCTCTAGTTGGGATGTTGTGCTGGCAAAt                                                                       >  2:649520/1‑140 (MQ=255)                                                                            cGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCacgacg                                                       >  2:608938/1‑140 (MQ=255)                                                                               catcatGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGgcg                                                    <  1:114297/140‑1 (MQ=255)                                                                                           tACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCt                                        <  2:331837/140‑1 (MQ=255)                                                                                                   gCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTg                                >  1:689274/1‑140 (MQ=255)                                                                                                   gCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTg                                >  2:111349/1‑140 (MQ=255)                                                                                                    cGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTgg                               <  2:396934/140‑1 (MQ=255)                                                                                                      aGCGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGcc                             >  1:465477/1‑140 (MQ=255)                                                                                                        cGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCg                           >  1:651142/1‑140 (MQ=255)                                                                                                        cGGATAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCg                           >  2:451596/1‑140 (MQ=255)                                                                                                           aTAATCATTAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCa                        <  1:504324/140‑1 (MQ=255)                                                                                                                   tAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                    <  1:488854/136‑1 (MQ=255)                                                                                                                   tAACTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                    >  2:488854/1‑136 (MQ=255)                                                                                                                     aCTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                    >  1:181059/1‑134 (MQ=255)                                                                                                                     aCTACCCCAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCg                    <  2:181059/134‑1 (MQ=255)                                                                                                                      cTACCCCAGCCCGGATCTGCTGCCTGACGCATGGTGGTTCGAGGACATTGATTTCTCTCAGTGGGATGTTTTGCTGGCAGATCTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCTCCAGGCGGGTGcga             >  2:674724/1‑140 (MQ=255)                                                                                                                         ccccAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga          >  1:200671/1‑140 (MQ=255)                                                                                                                         ccccAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga          <  1:608938/140‑1 (MQ=255)                                                                                                                         ccccAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga          >  2:730251/1‑140 (MQ=255)                                                                                                                          cccAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGAc         >  2:29563/1‑140 (MQ=255)                                                                                                                           ccAGCCCGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGAcc        >  1:89332/1‑140 (MQ=255)                                                                                                                                 cGGATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTg  <  1:272376/140‑1 (MQ=255)                                                                                                                                   gATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga          >  2:524713/1‑130 (MQ=255)                                                                                                                                   gATCTGCCGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGtgatga          <  1:524713/130‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   GCTGGCAGAGCTGGAATCCTGGGGCGTTAACACCCGTTACACCAAACGGTATAACCAGGCGAAATCTTCGCAATCCGCCATCATGGTGGATACCAAAGGCGAGCGGATAATCATTAACTACCCCAGCCCGGATCTGCTGCCTGACGCAGAGTGGTTGGAGGAAATTGATTTCTCTCAGTGGGATGTTGTGCTGGCAGATGTACGCTGGCACGACGGCGCTAAAAAAGCCTTCACCCTGGCCCGTCAGGCGGGTGTGATGACCGTTCTG  >  NC_000913/4073948‑4074215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑