Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,231	T→G	intergenic (‑18/+182)	yjcH ← / ← acs	conserved inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,231	0	T	G	100.0%	131.4 / NA	38	intergenic (‑18/+182)	yjcH/acs	conserved inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/21);  total (17/21)

AGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGT  >  NC_000913/4285096‑4285364                                                                                                                                        |                                                                                                                                      aGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATg                                                                                                                                   >  1:557696/1‑140 (MQ=255)  gCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGt                                                                                                                                  <  2:650466/140‑1 (MQ=255)   ccGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTa                                                                                                                                 >  2:365631/1‑140 (MQ=255)          aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTg                                                                                                                          <  2:128200/140‑1 (MQ=255)                ccAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTaa                                                                                                                    >  2:29268/1‑140 (MQ=255)                       aataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGc                                                                                                             <  1:5141/140‑1 (MQ=255)                       aataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGc                                                                                                             <  2:493559/140‑1 (MQ=255)                          aatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTcc                                                                                                          <  2:431954/140‑1 (MQ=255)                              gACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                      >  1:534561/1‑140 (MQ=255)                                 aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                                                                                                       >  1:316506/1‑136 (MQ=255)                                 aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                                                                                                       <  2:316506/136‑1 (MQ=255)                                 aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                                   >  2:383902/1‑140 (MQ=255)                                        tggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                            >  2:451720/1‑140 (MQ=255)                                        tggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg                                                                                            >  2:352388/1‑140 (MQ=255)                                         ggCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGt                                                                                           <  1:262884/140‑1 (MQ=255)                                          gCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGtt                                                                                          <  1:387045/140‑1 (MQ=255)                                          gCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGtt                                                                                          <  1:29268/140‑1 (MQ=255)                                             aaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc                                                                                       >  1:282201/1‑140 (MQ=255)                                                    gCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCggg                                                                                >  1:280060/1‑140 (MQ=255)                                                     ccGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGt                                                                               <  1:383902/140‑1 (MQ=255)                                                        tttttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTagc                                                                            >  1:495481/1‑138 (MQ=255)                                                          tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAAccc                                                                          <  2:223794/140‑1 (MQ=255)                                                          tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAAccc                                                                          <  1:352388/140‑1 (MQ=255)                                                               aCTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGcc                                                                     <  1:59954/140‑1 (MQ=255)                                                                  aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGaa                                                                  >  1:257909/1‑140 (MQ=255)                                                                  aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACG‑‑CGGGTAACCCTTGCCGAATg                                                                <  1:402057/140‑1 (MQ=255)                                                                        cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAgg                                                            <  1:451720/140‑1 (MQ=255)                                                                         tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGc                                                           <  1:365631/140‑1 (MQ=255)                                                                                 gcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAg                                                   >  2:330299/1‑140 (MQ=255)                                                                                          tctATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcg                                          <  2:60374/140‑1 (MQ=255)                                                                                                    gATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                <  2:280060/140‑1 (MQ=255)                                                                                                    gATAAATAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                <  2:534561/140‑1 (MQ=255)                                                                                                         aTAGTGCCATTCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaatc                           <  2:257909/140‑1 (MQ=255)                                                                                                                  ttCATCACAGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGa                  >  2:57495/1‑140 (MQ=255)                                                                                                                         caGATTCTCCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAATCAATGCCTGATGCGACg           >  2:728399/1‑140 (MQ=255)                                                                                                                               ctcCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg     >  1:529434/1‑140 (MQ=255)                                                                                                                               ctcCAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg     >  2:83528/1‑140 (MQ=255)                                                                                                                                  cAGAGGGAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAGCCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGt  <  2:495481/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGT  >  NC_000913/4285096‑4285364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑