Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,303,801	C→T	M103I (ATG→ATA)	mdtN ←	putative multidrug efflux pump membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,303,801	0	C	T	100.0%	91.6 / NA	28	M103I (ATG→ATA)	mdtN	putative multidrug efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

AAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAG  >  NC_000913/4303666‑4303926                                                                                                                                        |                                                                                                                              aaaaCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATaa                                                                                                                           <  2:645569/140‑1 (MQ=255)       ttcttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCt                                                                                                                     >  2:742170/1‑140 (MQ=255)         cttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATaa                                                                                                                           >  2:610258/1‑132 (MQ=255)         cttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATaa                                                                                                                           <  1:610258/132‑1 (MQ=255)               gTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAgc                                                                                                             >  1:448538/1‑140 (MQ=255)                   tGGCTCGGTGTGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGcgccg                                                                                                         <  2:688873/140‑1 (MQ=255)                                     tGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCtt                                                                                       >  2:266697/1‑140 (MQ=255)                                        aTCTGTGGCCTGTTTCGCGGCGGCACGGGGTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTGAACGCTGCGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCg                                                                                    <  2:390697/140‑1 (MQ=255)                                             tgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGa                                                                               <  1:139635/140‑1 (MQ=255)                                             tgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGa                                                                               <  1:164787/140‑1 (MQ=255)                                              ggCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAt                                                                              >  2:586249/1‑140 (MQ=255)                                               gCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAtt                                                                             >  1:511359/1‑140 (MQ=255)                                               gCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAtt                                                                             >  2:590198/1‑140 (MQ=255)                                               gCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAtt                                                                             >  2:505520/1‑140 (MQ=255)                                                        gcggcgGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTa                                                                    <  2:746060/140‑1 (MQ=255)                                                                   ggCTTTTTCTACCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGGCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGt                                                         <  2:72565/140‑1 (MQ=255)                                                                        tttCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATg                                                    <  1:421409/140‑1 (MQ=255)                                                                           cTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCgg                                                 <  2:204816/140‑1 (MQ=255)                                                                                   gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCaa                                         >  1:722306/1‑140 (MQ=255)                                                                                   gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCaa                                         <  1:266697/140‑1 (MQ=255)                                                                                    cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCaaa                                        <  1:742170/140‑1 (MQ=255)                                                                                       tAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCg                                     <  1:590198/140‑1 (MQ=255)                                                                                             aGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                               >  2:487004/1‑140 (MQ=255)                                                                                             aGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                               >  1:202563/1‑140 (MQ=255)                                                                                                 ggCACCAAACTGTTGCGCGTCACCGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGa                           <  1:586249/140‑1 (MQ=255)                                                                                                                cgcgTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCgg            >  2:306985/1‑140 (MQ=255)                                                                                                                  cgTCAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTg          >  1:110596/1‑140 (MQ=255)                                                                                                                     cAACGCTACGCTGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAcc       <  2:448538/140‑1 (MQ=255)                                                                                                                          ctacgctGGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAg  <  2:202563/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                              AAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAG  >  NC_000913/4303666‑4303926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑