Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,554	T→C	noncoding (73/76 nt)	pheU ←	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,554	0	T	C	100.0%	134.0 / NA	40	noncoding (73/76 nt)	pheU	tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/26);  total (14/26)

ATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAA  >  NC_000913/4362419‑4362690                                                                                                                                        |                                                                                                                                         atGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGccc                                                                                                                                      <  1:474421/140‑1 (MQ=255)  tGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCg                                                                                                                                     >  2:762212/1‑140 (MQ=255)  tGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAAGTTGGCGCCCg                                                                                                                                     >  1:773556/1‑140 (MQ=255)    cccGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGTGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGa                                                                                                                                   >  1:291262/1‑140 (MQ=255)            ttGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAAt                                                                                                                           <  1:800815/140‑1 (MQ=255)            ttGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAAt                                                                                                                           <  1:448478/140‑1 (MQ=255)            ttGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAAt                                                                                                                           <  2:473851/140‑1 (MQ=255)                 ggTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAAc                                                                                                                      <  2:582942/140‑1 (MQ=255)                  gTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTTGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAAcc                                                                                                                     <  2:773556/140‑1 (MQ=255)                       gtgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATc                                                                                                                          <  1:204227/130‑1 (MQ=255)                       gtgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATc                                                                                                                          >  2:204227/1‑130 (MQ=255)                           ggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACg                                                                                                            <  2:706657/140‑1 (MQ=255)                            gagaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACgg                                                                                                           >  2:141804/1‑140 (MQ=255)                              gaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACgggg                                                                                                         <  2:298316/140‑1 (MQ=255)                               aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAGCGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGa                                                                                                        >  2:208914/1‑140 (MQ=255)                               aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGa                                                                                                        >  1:665446/1‑140 (MQ=255)                               aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGa                                                                                                        >  2:124922/1‑140 (MQ=255)                                     gTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCa                                                                                                  <  2:232058/140‑1 (MQ=255)                                        gAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATc                                                                                               <  1:788693/140‑1 (MQ=255)                                         aGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATcc                                                                                              <  1:110217/140‑1 (MQ=255)                                               gTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGct                                                                                        >  1:626554/1‑140 (MQ=255)                                                                  aGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCg                                                                     >  2:187154/1‑140 (MQ=255)                                                                           aaaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACggg                                                            <  2:563324/140‑1 (MQ=255)                                                                           aaaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACggg                                                            <  2:291262/140‑1 (MQ=255)                                                                            aaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACgggg                                                           <  1:180266/140‑1 (MQ=255)                                                                            aaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACgggg                                                           <  2:421659/140‑1 (MQ=255)                                                                              tCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcg                                                         <  2:333547/140‑1 (MQ=255)                                                                                gTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCa                                                       <  1:187154/140‑1 (MQ=255)                                                                                  ggCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCAtt                                                     <  1:762212/140‑1 (MQ=255)                                                                                   gCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                                    >  2:551977/1‑140 (MQ=255)                                                                                       ccTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAAcc                                                <  2:99882/140‑1 (MQ=255)                                                                                                    aCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAt                                   <  1:124922/140‑1 (MQ=255)                                                                                                          ccccGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTAAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTc                             <  2:8242/138‑1 (MQ=255)                                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTc                             <  2:715630/140‑1 (MQ=255)                                                                                                             cGTGGAGGTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc                          <  1:208914/140‑1 (MQ=255)                                                                                                                    gTCCGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACTGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                   >  1:296704/1‑140 (MQ=255)                                                                                                                       cGTTTATATGAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAg                >  2:745045/1‑140 (MQ=255)                                                                                                                                gAATTTGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGa       <  1:551977/140‑1 (MQ=255)                                                                                                                                   tttGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTc    >  1:733070/1‑140 (MQ=255)                                                                                                                                   tttGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTc    >  1:265793/1‑140 (MQ=255)                                                                                                                                     tGGCGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCaa  <  1:745045/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         ATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAA  >  NC_000913/4362419‑4362690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑