Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,436,745	A→G	intergenic (‑180/‑10)	cpdB ← / → cysQ	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase/3'(2'),5'‑bisphosphate nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,436,745	0	A	G	100.0%	276.7 / NA	78	intergenic (‑180/‑10)	cpdB/cysQ	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase/3'(2'),5'‑bisphosphate nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/31);  total (47/31)

AATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGGATATTG  >  NC_000913/4436609‑4436881                                                                                                                                         |                                                                                                                                         aaTAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGt                                                                                                                                       >  2:659007/1‑140 (MQ=255) aaTAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGt                                                                                                                                       >  2:360565/1‑140 (MQ=255)  aTAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTg                                                                                                                                      >  2:446061/1‑140 (MQ=255)           ttacttaTAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGt                                                                                                                             <  2:141648/140‑1 (MQ=255)                     aaCAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGt                                                                                                                   >  1:90847/1‑140 (MQ=255)                     aaCAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGt                                                                                                                   >  1:413952/1‑140 (MQ=255)                       cAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTAt                                                                                                                 >  2:190401/1‑140 (MQ=255)                        aGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATg                                                                                                                >  2:624578/1‑140 (MQ=255)                        aGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATg                                                                                                                >  1:687309/1‑140 (MQ=255)                        aGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATg                                                                                                                >  2:559095/1‑140 (MQ=255)                        aGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATg                                                                                                                <  2:687309/140‑1 (MQ=255)                         gTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGc                                                                                                               >  1:252836/1‑140 (MQ=255)                          tGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGcc                                                                                                              >  1:767991/1‑140 (MQ=255)                          tGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGcc                                                                                                              >  1:507094/1‑140 (MQ=255)                          tGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGcc                                                                                                              <  2:703079/140‑1 (MQ=255)                          tGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGcc                                                                                                              >  1:231900/1‑140 (MQ=255)                           gaagaaTGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCa                                                                                                             >  2:402797/1‑140 (MQ=255)                            aagaaTGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCac                                                                                                            >  1:443418/1‑139 (MQ=255)                                    cacaATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACg                                                                                                    <  2:684940/140‑1 (MQ=255)                                        aTTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAAt                                                                                                <  2:507094/140‑1 (MQ=255)                                                cTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGa                                                                                        <  2:767991/140‑1 (MQ=255)                                                   tGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGc                                                                                     >  1:361431/1‑140 (MQ=255)                                                   tGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGc                                                                                     <  1:268891/140‑1 (MQ=255)                                                   tGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGc                                                                                     >  1:94038/1‑140 (MQ=255)                                                   tGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATACAGGCGATGc                                                                                     <  2:452763/140‑1 (MQ=255)                                                     aaaaTGATGACACTATCACAGTTGGCGTATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCa                                                                                   <  2:662005/140‑1 (MQ=255)                                                     aaaaTGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCa                                                                                   <  1:360565/140‑1 (MQ=255)                                                     aaaaTGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCa                                                                                   <  1:659007/140‑1 (MQ=255)                                                          gatgaCACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATg                                                                              <  1:402797/140‑1 (MQ=255)                                                          gatgaCACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATg                                                                              <  2:174862/140‑1 (MQ=255)                                                           atgaCACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGc                                                                             <  2:107155/140‑1 (MQ=255)                                                           atgaCACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGc                                                                             <  2:496731/140‑1 (MQ=255)                                                           atgaCACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGc                                                                             <  2:90847/140‑1 (MQ=255)                                                                    tCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTacg                                                                    >  1:780002/1‑140 (MQ=255)                                                                    tCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTacg                                                                    >  1:484711/1‑140 (MQ=255)                                                                    tCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTacg                                                                    >  2:182780/1‑140 (MQ=255)                                                                          ttGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGa                                                              <  2:413952/140‑1 (MQ=255)                                                                              cgcATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGaa                                                          <  2:710781/140‑1 (MQ=255)                                                                               gcATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGaaa                                                         <  2:367667/140‑1 (MQ=255)                                                                                   tcattAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGa                                                     <  1:190401/140‑1 (MQ=255)                                                                                     attAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATg                                                   <  1:559095/140‑1 (MQ=255)                                                                                             tAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGAcgtcgt                                           >  1:563854/1‑140 (MQ=255)                                                                                             tAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGAcgtcgt                                           >  1:817260/1‑140 (MQ=255)                                                                                             tAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGAcgtcgt                                           >  2:260397/1‑140 (MQ=255)                                                                                              aGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACgtcgtc                                          >  1:760716/1‑140 (MQ=255)                                                                                               gggTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCa                                         >  2:224833/1‑140 (MQ=255)                                                                                                ggTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATgg                                                  <  2:619819/130‑1 (MQ=255)                                                                                                ggTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATgg                                                  >  1:619819/1‑130 (MQ=255)                                                                                                ggTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAg                                        <  1:260397/140‑1 (MQ=255)                                                                                                     aaGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGAc                                                <  1:696686/127‑1 (MQ=255)                                                                                                     aaGTAAAACAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGAc                                                >  2:696686/1‑127 (MQ=255)                                                                                                         aaaaCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGa                               <  1:592705/140‑1 (MQ=255)                                                                                                          aaaCAATAAGTTAACGCCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGAc                              >  2:605056/1‑140 (MQ=255)                                                                                                          aaaCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGAc                              >  1:12141/1‑140 (MQ=255)                                                                                                           aaCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACa                             >  2:49873/1‑140 (MQ=255)                                                                                                           aaCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACa                             >  2:647153/1‑140 (MQ=255)                                                                                                           aaCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACa                             >  1:512652/1‑140 (MQ=255)                                                                                                            aCAATAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACaa                            >  2:11147/1‑140 (MQ=255)                                                                                                                tAAGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtct                        <  1:224833/140‑1 (MQ=255)                                                                                                                 aaGTTAACACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtctc                       >  2:520688/1‑140 (MQ=255)                                                                                                                      aaCACCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTa                  >  1:391823/1‑140 (MQ=255)                                                                                                                        cacCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtct                        <  1:169905/132‑1 (MQ=255)                                                                                                                        cacCGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATtct                        >  2:169905/1‑132 (MQ=255)                                                                                                                          ccGCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACgg              <  2:12141/140‑1 (MQ=255)                                                                                                                            gCTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCa            >  2:466199/1‑140 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACg               <  2:333328/136‑1 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACg               >  1:333328/1‑136 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAg           <  2:391823/140‑1 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAg           >  2:212721/1‑140 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAg           >  1:745522/1‑140 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAg           >  1:689300/1‑140 (MQ=255)                                                                                                                             cTCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAg           >  1:317915/1‑140 (MQ=255)                                                                                                                              tCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGc          >  1:690361/1‑140 (MQ=255)                                                                                                                              tCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGc          >  1:18676/1‑140 (MQ=255)                                                                                                                              tCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGc          >  1:390864/1‑140 (MQ=255)                                                                                                                              tCACAGAGACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGc          <  1:520688/140‑1 (MQ=255)                                                                                                                                  agagACGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGGat      >  2:250795/1‑140 (MQ=255)                                                                                                                                      aCGGGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGGATATTg  >  2:473330/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         AATAAACGTCTTACTTATAGAACAGTGAAGAATGCCACAATTTTACGCTTTGAAAATGATGACACTATCACAGTTGGCGCATTCATTAACGATAGGGTATAAGTAAAACAATAAGTTAACACCGCTCACAGAGACGAGGTGGAGAAATGTTAGATCAAGTATGCCAGCTTGCACGGAATGCAGGCGATGCCATTATGCAGGTCTACGACGGGACGAAACCGATGGACGTCGTCAGCAAAGCGGACAATTCTCCGGTAACGGCAGCGGATATTG  >  NC_000913/4436609‑4436881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑