Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,446,396	A→G	D762G (GAC→GGC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,446,396	0	A	G	100.0%	130.5 / NA	38	D762G (GAC→GGC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/22);  total (16/22)

GCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAT  >  NC_000913/4446259‑4446521                                                                                                                                          |                                                                                                                              gCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGgca                                                                                                                             >  1:820967/1‑140 (MQ=255)   aaaCTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGcacc                                                                                                                           >  1:323646/1‑140 (MQ=255)         tCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGaaa                                                                                                                     >  2:745140/1‑140 (MQ=255)           gATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAaga                                                                                                                   <  2:323646/140‑1 (MQ=255)                gggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              <  2:820967/140‑1 (MQ=255)                gggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              <  1:745140/140‑1 (MQ=255)                 ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              <  2:331458/139‑1 (MQ=255)                 ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              >  1:331458/1‑139 (MQ=255)                 ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCt                                                                                                             >  2:682372/1‑140 (MQ=255)                        aaGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              >  2:546454/1‑132 (MQ=255)                        aaGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGc                                                                                                              <  1:546454/132‑1 (MQ=255)                         aGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAgg                                                                                                     <  2:789937/140‑1 (MQ=255)                          gggCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAggg                                                                                                    <  1:763314/140‑1 (MQ=255)                          gggCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAggg                                                                                                    <  2:697110/140‑1 (MQ=255)                              gTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAggg                                                                                                    <  1:753684/136‑1 (MQ=255)                              gTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAggg                                                                                                    >  2:753684/1‑136 (MQ=255)                                  ggtggtGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCa                                                                                            <  1:160744/140‑1 (MQ=255)                                           tctcAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCa                                                                                            >  1:290743/1‑131 (MQ=255)                                           tctcAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCa                                                                                            <  2:290743/131‑1 (MQ=255)                                            ctcAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCggg                                                                                  <  2:356436/140‑1 (MQ=255)                                              cAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGc                                                                                >  1:42442/1‑140 (MQ=255)                                                   gCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAAc                                                                           >  2:456161/1‑140 (MQ=255)                                                     ttCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                                                         >  2:68747/1‑140 (MQ=255)                                                      tCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTg                                                                        >  2:136754/1‑140 (MQ=255)                                                       cGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGc                                                                       <  2:484997/140‑1 (MQ=255)                                                                cATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGc                                                              <  1:682372/140‑1 (MQ=255)                                                                   gCTGAAACCGTTTATGCCAGAAACCGCTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCaaa                                                           <  1:783831/140‑1 (MQ=255)                                                                            gTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGc                                                              >  1:154264/1‑128 (MQ=255)                                                                            gTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGc                                                              <  2:154264/128‑1 (MQ=255)                                                                             tttATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGa                                                 >  1:492061/1‑140 (MQ=255)                                                                                       aaaCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTg                                       >  2:555239/1‑140 (MQ=255)                                                                                       aaaCCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTg                                       >  2:587135/1‑140 (MQ=255)                                                                                                       cGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGa                       <  1:68747/140‑1 (MQ=255)                                                                                                                 aCGGGTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCt             >  2:542933/1‑140 (MQ=255)                                                                                                                   gggTAAAGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGa           <  1:542933/140‑1 (MQ=255)                                                                                                                         aGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcgg     <  1:587135/140‑1 (MQ=255)                                                                                                                         aGCGGACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGAcggcgg     <  1:555239/140‑1 (MQ=255)                                                                                                                            ggACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAt  <  2:100620/140‑1 (MQ=255)                                                                                                                            ggACGTTGCCTGGGGCACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAt  <  1:136754/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                              GCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAAT  >  NC_000913/4446259‑4446521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑