Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,534,809	T→C	intergenic (‑134/‑206)	yjhZ ← / → yjhR	KpLE2 phage‑like element; putative acetyltransferase YjhZ/KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,534,809	0	T	C	100.0%	208.9 / NA	60	intergenic (‑134/‑206)	yjhZ/yjhR	KpLE2 phage‑like element; putative acetyltransferase YjhZ/KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

TGACAATGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTA  >  NC_000913/4534680‑4534942                                                                                                                                  |                                                                                                                                      tgACAATGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAg                                                                                                                             >  1:707875/1‑140 (MQ=255)  gACAATGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAgg                                                                                                                            >  2:413516/1‑139 (MQ=255)      aTGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTgcag                                                                                                                        <  2:786970/140‑1 (MQ=255)       tGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTgcagc                                                                                                                       >  2:330795/1‑140 (MQ=255)       tGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTgcagc                                                                                                                       >  2:814903/1‑140 (MQ=255)             aTTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTgg                                                                                                                 <  1:169901/140‑1 (MQ=255)                   tatCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGt                                                                                                           >  1:461150/1‑140 (MQ=255)                    atCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTgg                                                                                                                 >  1:243999/1‑133 (MQ=255)                    atCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTgg                                                                                                                 <  2:243999/133‑1 (MQ=255)                        cgAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGc                                                                                                      >  1:403364/1‑140 (MQ=255)                        cgAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGc                                                                                                      >  2:665524/1‑140 (MQ=255)                           gCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAgg                                                                                                   <  2:217717/140‑1 (MQ=255)                               gtgtAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGc                                                                                               >  1:338591/1‑140 (MQ=255)                                    aGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGt                                                                                          >  2:115317/1‑140 (MQ=255)                                        cTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCaa                                                                                      <  2:352631/140‑1 (MQ=255)                                        cTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCaa                                                                                      <  2:707875/140‑1 (MQ=255)                                         tCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAAc                                                                                     >  2:761228/1‑140 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTc                                                                                        >  1:199011/1‑136 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTc                                                                                        <  2:199011/136‑1 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGc                                                                                               >  1:813242/1‑129 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGc                                                                                               <  2:813242/129‑1 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAAct                                                                                    >  1:472826/1‑140 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAAct                                                                                    >  2:666627/1‑140 (MQ=255)                                          cccAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAAct                                                                                    >  2:819091/1‑140 (MQ=255)                                            cAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAActct                                                                                  <  1:84768/140‑1 (MQ=255)                                            cAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAActct                                                                                  <  1:819091/140‑1 (MQ=255)                                            cAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAActct                                                                                  <  2:403364/140‑1 (MQ=255)                                            cAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAActct                                                                                  <  2:86506/140‑1 (MQ=255)                                             aGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACtctc                                                                                 >  2:373370/1‑140 (MQ=255)                                               cAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCAc                                                                               <  1:682825/140‑1 (MQ=255)                                                  gACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTAc                                                                            <  1:372246/140‑1 (MQ=255)                                                              aatTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                                >  1:369853/1‑140 (MQ=255)                                                                     tgtAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCg                                                         >  1:825648/1‑140 (MQ=255)                                                                     tgtAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCg                                                         >  1:108681/1‑140 (MQ=255)                                                                         gcTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcg                                                     <  2:716231/139‑1 (MQ=255)                                                                         aCTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcg                                                     <  1:330795/140‑1 (MQ=255)                                                                         aCTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcg                                                     <  2:783073/140‑1 (MQ=255)                                                                          cTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                                    >  1:204951/1‑140 (MQ=255)                                                                           tGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                                   <  1:81382/140‑1 (MQ=255)                                                                             cccACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGa                                                 <  1:373370/140‑1 (MQ=255)                                                                               cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAt                                               >  1:119822/1‑140 (MQ=255)                                                                               cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAt                                               >  1:337249/1‑140 (MQ=255)                                                                                             gCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                                 >  2:31258/1‑140 (MQ=255)                                                                                             gCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                                 >  2:259477/1‑140 (MQ=255)                                                                                                    aGGCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                          <  2:825648/140‑1 (MQ=255)                                                                                                      gCGGTCACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCt                        >  2:19109/1‑140 (MQ=255)                                                                                                           cACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                          >  1:36700/1‑133 (MQ=255)                                                                                                           cACCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                          <  2:36700/133‑1 (MQ=255)                                                                                                            aCCGTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa                  >  2:698057/1‑140 (MQ=255)                                                                                                               gTATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATa               <  1:259477/140‑1 (MQ=255)                                                                                                                tATTCGATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATaa              >  1:627641/1‑140 (MQ=255)                                                                                                                     gATAATAAATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAcatca         <  1:814903/140‑1 (MQ=255)                                                                                                                       taataaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACatcatc       <  1:672453/140‑1 (MQ=255)                                                                                                                       taataaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACatcatc       >  1:769208/1‑140 (MQ=255)                                                                                                                        aataaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCg      >  1:370327/1‑140 (MQ=255)                                                                                                                        aataaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCg      >  1:76358/1‑140 (MQ=255)                                                                                                                        aataaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCg      >  1:31569/1‑140 (MQ=255)                                                                                                                          taaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGac    <  2:461150/140‑1 (MQ=255)                                                                                                                          taaATGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGac    <  2:204951/140‑1 (MQ=255)                                                                                                                            aaTGGGCCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGacta  <  2:303813/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TGACAATGGCCAATTGAATATCGCGAGCAAGTGTAAGAACTCCCAGCAAGACCCTGTAAATAATTCTGTGTAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCATCGACTA  >  NC_000913/4534680‑4534942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑