Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,563,985	A→G	D235D (GAT→GAC)	yjiL ←	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,563,985	0	A	G	100.0%	131.1 / NA	40	D235D (GAT→GAC)	yjiL	putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

GCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCG  >  NC_000913/4563851‑4564119                                                                                                                                       |                                                                                                                                       gCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAgg                                                                                                                                   >  1:434754/1‑140 (MQ=255)  cccGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGa                                                                                                                                  <  2:782324/140‑1 (MQ=255)   ccGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGAt                                                                                                                                 <  1:593061/140‑1 (MQ=255)   ccGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGAt                                                                                                                                 <  2:357781/140‑1 (MQ=255)          ctgcAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGGCCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGGAGGGGAtt                                                                                                                          >  2:334419/1‑140 (MQ=255)             cAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTAc                                                                                                                       >  2:349806/1‑140 (MQ=255)               aCGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCg                                                                                                                     <  1:349806/140‑1 (MQ=255)                    ttGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCAtt                                                                                                                <  2:489664/140‑1 (MQ=255)                       cGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGc                                                                                                             <  2:712/140‑1 (MQ=255)                        gggTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCa                                                                                                            <  2:114016/140‑1 (MQ=255)                                        gACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCAt                                                                                            <  1:796959/140‑1 (MQ=255)                                         aCCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATc                                                                                           >  1:698704/1‑140 (MQ=255)                                              ggAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGc                                                                                      >  2:658816/1‑140 (MQ=255)                                                  tGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAAc                                                                                  >  2:826451/1‑140 (MQ=255)                                                   gAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACt                                                                                 >  2:561282/1‑140 (MQ=255)                                                         aaaataaaaaCTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGc                                                                           <  1:357297/140‑1 (MQ=255)                                                           aataaaaaCTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCaa                                                                         <  1:463614/140‑1 (MQ=255)                                                              aaaaaCTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGa                                                                      <  1:658816/140‑1 (MQ=255)                                                                     ctTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGc                                                               <  1:428958/140‑1 (MQ=255)                                                                     ctTCCATCGGCGTGTCCTCACTCGTTAACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGc                                                               <  1:334419/140‑1 (MQ=255)                                                                            cGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGt                                                        >  2:288113/1‑140 (MQ=255)                                                                                   tcatCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCCAATTGCGCATCAGGACTGGTATTTTCCGCCATTCCCTCGTGAGAGTCCATGATCCAAGCAACCTCCTCGCCATTATCAACACCACCAATaaaacca                                                 >  2:523832/4‑136 (MQ=255)                                                                                   tCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGa                                                 >  2:24856/1‑140 (MQ=255)                                                                                     cTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAAt                                               >  1:356865/1‑140 (MQ=255)                                                                                            gTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATctgg                                           >  1:468903/1‑134 (MQ=255)                                                                                            gTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATctgg                                           <  2:468903/137‑4 (MQ=255)                                                                                             ttGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                          >  2:131638/1‑137 (MQ=255)                                                                                             ttGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCtt                                       >  1:67379/1‑140 (MQ=255)                                                                                             ttGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCtt                                       >  1:537385/1‑140 (MQ=255)                                                                                             ttGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCtt                                       <  1:288113/140‑1 (MQ=255)                                                                                             ttGACAAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGgcg                                          <  1:131638/137‑1 (MQ=255)                                                                                                         cgccgcGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGAc                           <  1:826451/140‑1 (MQ=255)                                                                                                          gccgcGCCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACg                          <  1:24856/140‑1 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCtt                                       >  1:701685/1‑123 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCtt                                       <  2:701685/123‑1 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTca                                     >  2:397575/1‑125 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTca                                     <  1:397575/125‑1 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCa                      >  1:551986/1‑140 (MQ=255)                                                                                                              cgcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCa                      <  2:67379/140‑1 (MQ=255)                                                                                                               gcCAATTGCGCCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCaa                     >  2:782313/1‑140 (MQ=255)                                                                                                                      gcgcCAGCAAATTGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAAtt              <  1:373454/140‑1 (MQ=255)                                                                                                                                  tGCGCGTCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCcg  >  2:431556/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GCCCGCTGCCTGCAACGCTTTGCGGGTTTGTATGACGCCGACCGTGGAGTGAAATAAAAATAAAAACTCTTTCATCGGCGTGTCCTCACTCGTTGACCAATTACCGCCGCGCCAATTGCGCCAGCAAATTGCGCATCAGGATGGGTATTTACCGGCATTCGCAGGTGAGATTCCAGCATCCGGGCAAACTTCTGGCAATGACTAACGCCACCAGTAAACAGAATCGGCGCTTCACAGGAGAGACGAGCAATGAAATTGGCACTCCTCCG  >  NC_000913/4563851‑4564119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑