Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,375	A→G	V302A (GTG→GCG)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,375	0	A	G	100.0%	161.5 / NA	47	V302A (GTG→GCG)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/23);  total (24/23)

GATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATG  >  NC_000913/4590239‑4590509                                                                                                                                         |                                                                                                                                       gaTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcgcca                                                                                                                                     <  1:496321/140‑1 (MQ=255)  aTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCGTGGCAGGCATTTTCAGTTCCGGGTTCAGcgccac                                                                                                                                    >  1:577903/1‑140 (MQ=255)  aTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcgccac                                                                                                                                    >  2:91277/1‑140 (MQ=255)  aTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcgccac                                                                                                                                    >  1:690194/1‑140 (MQ=255)  aTCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGcgccac                                                                                                                                    >  2:164941/1‑140 (MQ=255)       cgcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATAc                                                                                                                               >  2:466754/1‑140 (MQ=255)        gcgTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATAcc                                                                                                                              <  2:690194/140‑1 (MQ=255)                           caccacAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATa                                                                                                           >  2:82847/1‑140 (MQ=255)                                   gCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGAt                                                                                                   >  2:114267/1‑140 (MQ=255)                                        ggTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCa                                                                                              >  1:9516/1‑140 (MQ=255)                                           gatgaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGa                                                                                           <  1:85931/140‑1 (MQ=255)                                            atgaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCGTGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGaa                                                                                          <  2:577903/140‑1 (MQ=255)                                             tgaACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAg                                                                                         >  1:291932/1‑140 (MQ=255)                                                 cAGGAACGAGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTg                                                                                     >  2:719445/1‑140 (MQ=255)                                                   ggAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGt                                                                                       >  2:265471/1‑136 (MQ=255)                                                   ggAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGt                                                                                       <  1:265471/136‑1 (MQ=255)                                                   ggAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGcc                                                                                   >  1:341030/1‑140 (MQ=255)                                                         ggAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAg                                                                             >  2:409544/1‑140 (MQ=255)                                                         ggAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAg                                                                             >  2:377797/1‑140 (MQ=255)                                                           aaCAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTc                                                                           >  1:190589/1‑140 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATCCCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  1:604248/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  2:632864/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  2:341030/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  1:114267/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  2:9516/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  1:466754/140‑1 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        >  1:123854/1‑140 (MQ=255)                                                              agagCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcg                                                                        <  1:377797/140‑1 (MQ=255)                                                                agCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTcgcgcg                                                                      <  2:589751/140‑1 (MQ=255)                                                                  cgcCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGt                                                                    >  2:287701/1‑140 (MQ=255)                                                                    ccTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGc                                                                  <  1:398840/140‑1 (MQ=255)                                                                    ccTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGc                                                                  >  2:721640/1‑140 (MQ=255)                                                                     cTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCg                                                                 >  2:799420/1‑140 (MQ=255)                                                                        tCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAgg                                                              <  1:799420/140‑1 (MQ=255)                                                                             aGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAg                                                         <  1:721640/140‑1 (MQ=255)                                                                              gCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGc                                                        <  2:123854/140‑1 (MQ=255)                                                                               cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCa                                                          <  1:648758/137‑1 (MQ=255)                                                                               cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCa                                                          >  2:648758/1‑137 (MQ=255)                                                                               cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGcc                                                       >  2:405945/1‑140 (MQ=255)                                                                               cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGcc                                                       >  1:204705/1‑140 (MQ=255)                                                                                 ggCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCcac                                                     >  1:624904/1‑140 (MQ=255)                                                                                          cGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGgcagc                                            >  1:552054/1‑140 (MQ=255)                                                                                            tCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGcagcag                                          >  2:707224/1‑140 (MQ=255)                                                                                                          cATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                            >  2:37913/1‑140 (MQ=255)                                                                                                          cATGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                            <  1:37913/140‑1 (MQ=255)                                                                                                           aTGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGc                           >  2:155512/1‑140 (MQ=255)                                                                                                            tGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTTCGGAAACAAACGCa                          >  1:71181/1‑140 (MQ=255)                                                                                                            tGGCAGGCATTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCa                          <  1:409544/140‑1 (MQ=255)                                                                                                                    aTTTTCAGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAAt                  <  1:555886/140‑1 (MQ=255)                                                                                                                          aGTTCCGGGTTCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcgc            <  1:405945/140‑1 (MQ=255)                                                                                                                                    tCAGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAAtg  <  1:164941/140‑1 (MQ=255)                                                                                                                                      aGCGCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGaa         <  2:303001/131‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GATCAGCGCGTGGAAGCCAGATACCGCACCACAGGCGATGGTGATGAACAGGAACGGGAACAGAGCGCCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATG  >  NC_000913/4590239‑4590509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑