Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	409,791	A→G	S77P (TCT→CCT)	rdgC ←	nucleoid‑associated protein RdgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	409,791	0	A	G	100.0%	143.5 / NA	41	S77P (TCT→CCT)	rdgC	nucleoid‑associated protein RdgC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/24);  total (17/24)

GCTGAAAGCACGCGGCAGCAGAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAA  >  NC_000913/409657‑409926                                                                                                                                       |                                                                                                                                        gCTGAAAGCACGCGGCAGCAGAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGgga                                                                                                                                    <  1:285053/140‑1 (MQ=255)   tGAAAGCACGCGGCAGCAGAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGggagg                                                                                                                                  <  2:471122/140‑1 (MQ=255)                 agcagAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCttt                                                                                                                    >  1:598532/1‑140 (MQ=255)                 agcagAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCttt                                                                                                                    >  1:83485/1‑140 (MQ=255)                   cagAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTgc                                                                                                                  >  1:690564/1‑140 (MQ=255)                    agagagTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTgcg                                                                                                                 <  2:395630/140‑1 (MQ=255)                          tGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCa                                                                                                           >  2:108894/1‑140 (MQ=255)                             agcaCTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGAt                                                                                                        <  1:661509/140‑1 (MQ=255)                                    cGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAtt                                                                                                 <  1:31585/140‑1 (MQ=255)                                    cGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAtt                                                                                                 >  1:449757/1‑140 (MQ=255)                                        tttCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAc                                                                                             <  1:190357/140‑1 (MQ=255)                                         ttCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAcc                                                                                            >  2:656685/1‑140 (MQ=255)                                          tCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa                                                                                           <  2:449757/140‑1 (MQ=255)                                          tCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACACGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCa                                                                                           <  1:381199/140‑1 (MQ=255)                                                   cTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACg                                                                                  <  1:737721/140‑1 (MQ=255)                                                        tCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGt                                                                             <  2:342464/140‑1 (MQ=255)                                                        tCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGt                                                                             <  2:487842/140‑1 (MQ=255)                                                                 ttcAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATc                                                                    <  1:268333/140‑1 (MQ=255)                                                                     gCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACCATTTGACCATTGGCAACCTGCGTTTACGCAACGCtt                                                                >  2:759633/1‑139 (MQ=255)                                                                      cTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTa                                                                           <  1:88894/128‑1 (MQ=255)                                                                      cTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTa                                                                           >  2:88894/1‑128 (MQ=255)                                                                      cTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCtgt                                                               <  2:598532/140‑1 (MQ=255)                                                                                tGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCAt                                                     >  1:559539/1‑140 (MQ=255)                                                                                         tCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAAc                                            >  1:294418/1‑140 (MQ=255)                                                                                                 ggCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCa                                    <  1:108894/140‑1 (MQ=255)                                                                                                    gATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCt                                 <  1:368068/140‑1 (MQ=255)                                                                                                    gATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCt                                 <  2:83485/140‑1 (MQ=255)                                                                                                        ttCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGc                             >  1:769070/1‑140 (MQ=255)                                                                                                        ttCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGc                             >  1:528251/1‑140 (MQ=255)                                                                                                         tCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGcc                            <  1:122778/140‑1 (MQ=255)                                                                                                         tCGCTTCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCACAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGcc                            <  2:690564/140‑1 (MQ=255)                                                                                                             ttCCAGCGCCTGTTTAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGt                        <  2:559539/140‑1 (MQ=255)                                                                                                                          ttAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCa           <  2:294418/140‑1 (MQ=255)                                                                                                                          ttAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCa           <  2:651046/140‑1 (MQ=255)                                                                                                                          ttAATCACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCa           <  2:769070/140‑1 (MQ=255)                                                                                                                               cACCGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATgggg      >  1:438522/1‑140 (MQ=255)                                                                                                                                  cGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCa           >  2:448433/1‑132 (MQ=255)                                                                                                                                  cGGAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCa           <  1:448433/132‑1 (MQ=255)                                                                                                                                   ggAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTaaa  >  2:293657/1‑140 (MQ=255)                                                                                                                                   ggAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTaaa  >  1:485309/1‑140 (MQ=255)                                                                                                                                   ggAGGCGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTaaa  >  1:17049/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        GCTGAAAGCACGCGGCAGCAGAGAGTGCAGCACTTCGTCTTTCAGCGAATCTTTTTCGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATTGGCAACGTGCGTTAACGCATCGCTGTGCGATCCCATCGGAGGAACCCAGCCCATCTTCGCCATGTCCTGGCTGCCGCATGGGGTAAA  >  NC_000913/409657‑409926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑