Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	417,596	C→T	P152L (CCG→CTG)	phoB →	DNA‑binding transcriptional dual regulator PhoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	417,596	0	C	T	100.0%	157.6 / NA	48	P152L (CCG→CTG)	phoB	DNA‑binding transcriptional dual regulator PhoB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/30);  total (18/30)

TCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGT  >  NC_000913/417463‑417725                                                                                                                                      |                                                                                                                                  tCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGga                                                                                                                             >  2:737946/1‑140 (MQ=255)     cGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATg                                                                                                                         >  2:359293/1‑140 (MQ=255)            gCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGAATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGa                                                                                                                  <  1:737946/140‑1 (MQ=255)             cTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGTTGCTGGAGATGGGGCCGAc                                                                                                                 <  2:351070/140‑1 (MQ=255)                    cgcgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGGAGAGGTGCTGGGGGAGGGGGCGACAGAAatt                                                                                                          >  2:813005/1‑140 (MQ=255)                       gAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTaaa                                                                                                       <  1:399292/140‑1 (MQ=255)                       gAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTaaa                                                                                                       <  1:517355/140‑1 (MQ=255)                                cGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCAc                                                                                              <  1:593497/140‑1 (MQ=255)                                                    ccAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGa                                                                          >  1:434231/1‑140 (MQ=255)                                                       aTGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGAAAAGCTGCTGGGGGTGGGGGCGACAGAATTTAAAATGCTGCACCTTTTTTTGACGCATCCCGGgcg                                                                       >  2:702763/1‑140 (MQ=255)                                                              tGGAAGAGGTGATTGAGATGCA‑GGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGGGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTTTGACGCATCCTGAGCGCGGGGACa                                                               >  2:709268/1‑140 (MQ=255)                                                                        gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                      >  1:442614/1‑140 (MQ=255)                                                                        gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                      >  1:261996/1‑140 (MQ=255)                                                                        gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                      <  1:702763/140‑1 (MQ=255)                                                                        gATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagc                                                      <  2:69972/140‑1 (MQ=255)                                                                         aTTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagca                                                     <  2:132787/140‑1 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGcc                                                            <  2:256326/132‑1 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGcc                                                            >  1:256326/1‑132 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGcc                                                            >  1:798791/1‑132 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGcc                                                            <  2:798791/132‑1 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagcag                                                    <  1:756293/140‑1 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagcag                                                    <  2:434231/140‑1 (MQ=255)                                                                          ttGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagcag                                                    <  2:751806/140‑1 (MQ=255)                                                                                tGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTa                                              >  2:719112/1‑140 (MQ=255)                                                                                  cAGGGATTAAGTCCCGCCCCGCAATCTCACCGAGTGATGGCGGGGGAAGAGCGGCTGGGGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTTTGACGCTTCCTGAGGGGGGGTAAAGCCGCGAGCACCTTTTata                                            >  2:766984/1‑138 (MQ=255)                                                                                   aGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAAc                                           <  2:261996/140‑1 (MQ=255)                                                                                       aTTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACg                                       >  1:246181/1‑140 (MQ=255)                                                                                        ttAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGt                                      >  2:419859/1‑140 (MQ=255)                                                                                           aGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTg                                   <  2:373687/140‑1 (MQ=255)                                                                                               tcGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGa                               <  1:813005/140‑1 (MQ=255)                                                                                               tcGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTGAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGa                               >  2:566608/1‑140 (MQ=255)                                                                                                 gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCAc                                        >  1:786313/1‑129 (MQ=255)                                                                                                 gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCAc                                        <  2:786313/129‑1 (MQ=255)                                                                                                 gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAAc                             >  1:518310/1‑140 (MQ=255)                                                                                                 gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAAc                             <  1:766984/140‑1 (MQ=255)                                                                                                     cGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                         <  1:359293/140‑1 (MQ=255)                                                                                                     cGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAAc                         <  1:419859/140‑1 (MQ=255)                                                                                                      gACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTa                           >  1:689540/1‑137 (MQ=255)                                                                                                      gACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTa                           <  2:689540/137‑1 (MQ=255)                                                                                                       aCATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACgt                       >  1:339674/1‑140 (MQ=255)                                                                                                       aCATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACgt                       <  1:566608/140‑1 (MQ=255)                                                                                                         aTCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACgtgt                     <  2:339674/140‑1 (MQ=255)                                                                                                         aTCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACgtgt                     <  2:246181/140‑1 (MQ=255)                                                                                                              aCCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTAtgtg                <  2:442614/140‑1 (MQ=255)                                                                                                                 gAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGaa             >  2:821477/1‑140 (MQ=255)                                                                                                                  aGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAACAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAg            >  2:769184/1‑140 (MQ=255)                                                                                                                     gATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGGCTGGGGAACTAACGTGTATGTGGAAAAcc         >  2:321266/1‑140 (MQ=255)                                                                                                                     gATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAAACACGTCTGGGGGACTAACGGGTATGTGGAAGAcc         >  2:825108/1‑140 (MQ=255)                                                                                                                      aTGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCg        <  2:677003/140‑1 (MQ=255)                                                                                                                      aTGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCg        >  2:221912/1‑140 (MQ=255)                                                                                                                        ggcgggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCa      <  1:769184/140‑1 (MQ=255)                                                                                                                          cgggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACg    <  1:821477/140‑1 (MQ=255)                                                                                                                            ggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGt  <  2:518310/140‑1 (MQ=255)                                                                                                                            ggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGt  <  1:221912/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                  TCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGT  >  NC_000913/417463‑417725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑