Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	428,460	A→G	I272V (ATT→GTT)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,460	0	A	G	100.0%	150.6 / NA	43	I272V (ATT→GTT)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/20);  total (23/20)

CATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGATTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTTGTG  >  NC_000913/428336‑428588                                                                                                                             |                                                                                                                                 cATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACgg                                                                                                                   >  1:697349/1‑140 (MQ=255) cATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACgg                                                                                                                   >  1:14837/1‑140 (MQ=255) cATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACgg                                                                                                                   <  2:405208/140‑1 (MQ=255)    tAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCaa                                                                                                                <  2:820990/140‑1 (MQ=255)      atatCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACg                                                                                                              >  1:323450/1‑140 (MQ=255)       tatCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACAGCAACGc                                                                                                             <  1:575019/140‑1 (MQ=255)           cTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGa                                                                                                         >  1:438483/1‑140 (MQ=255)           cTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGa                                                                                                         >  1:776378/1‑140 (MQ=255)            tGCGTAACCGTGTAAACCAACTTGGCGTCGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGaa                                                                                                        >  1:235106/1‑140 (MQ=255)                  aCCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGt                                                                                                  >  2:466921/1‑140 (MQ=255)                   ccGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTc                                                                                                 <  2:74043/140‑1 (MQ=255)                   ccGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTc                                                                                                 <  2:323450/140‑1 (MQ=255)                     gtgtAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTg                                                                                               >  1:530884/1‑140 (MQ=255)                       gtAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGt                                                                                             <  2:530884/140‑1 (MQ=255)                        tAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTa                                                                                            >  1:201240/1‑140 (MQ=255)                             cAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAacac                                                                                       <  2:776378/140‑1 (MQ=255)                                              gCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCg                                                                      >  2:497546/1‑140 (MQ=255)                                                        cAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATc                                                            <  2:14837/140‑1 (MQ=255)                                                        cAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATc                                                            <  2:293270/140‑1 (MQ=255)                                                            gTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGt                                                        <  2:438483/140‑1 (MQ=255)                                                             tCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTc                                                       <  1:204943/140‑1 (MQ=255)                                                               aGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTcgc                                                     >  2:351272/1‑140 (MQ=255)                                                                       gACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCggg                                             <  2:784260/140‑1 (MQ=255)                                                                                  tgttgAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCC‑GTCGCGTACCGGGCGACTCTGAAGt                                 >  1:451685/1‑140 (MQ=255)                                                                                      gAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGActc                                       <  1:525469/131‑1 (MQ=255)                                                                                      gAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGActc                                       >  2:525469/1‑131 (MQ=255)                                                                                       aaCTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGaaa                             <  1:466921/140‑1 (MQ=255)                                                                                            ccAGGTATTCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGAc                        >  2:434591/1‑140 (MQ=255)                                                                                                    tCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAg                <  2:201240/140‑1 (MQ=255)                                                                                                    tCAGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAg                >  2:798164/1‑140 (MQ=255)                                                                                                      aGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGaa                 <  2:681832/137‑1 (MQ=255)                                                                                                      aGGACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGaa                 >  1:681832/1‑137 (MQ=255)                                                                                                       ggACACTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCC‑GTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCa            <  2:451685/140‑1 (MQ=255)                                                                                                          cacTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGc          >  1:516867/1‑140 (MQ=255)                                                                                                           acTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGa                         <  2:354240/124‑1 (MQ=255)                                                                                                           acTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGa                         >  1:354240/1‑124 (MQ=255)                                                                                                           acTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGcc         >  2:304795/1‑140 (MQ=255)                                                                                                           acTGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGcc         >  1:138377/1‑140 (MQ=255)                                                                                                             tGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAg       <  1:497546/140‑1 (MQ=255)                                                                                                             tGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAg       >  1:507655/1‑140 (MQ=255)                                                                                                             tGCGCGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAg       >  2:389542/1‑140 (MQ=255)                                                                                                              gcgcGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGt      >  2:806819/1‑140 (MQ=255)                                                                                                              gcgcGTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGt      >  1:141694/1‑140 (MQ=255)                                                                                                                  gTGCGAAAGAGGTTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTtgtg  <  2:697349/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                 CATTAATATCCTGCGTAACCGTGTAAACCAACTTGGCGTGGCGGAGCCGGTGGTTCAGCGTCAGGGTGCTGACCGTATCGTTGTTGAACTGCCAGGTATTCAGGACACTGCGCGTGCGAAAGAGATTCTGGGTGCGACGGCAACGCTGGAATTCCGTCTGGTAAACACCAACGTTGACCAGGCCGCTGCGGCATCCGGTCGCGTACCGGGCGACTCTGAAGTGAAACAGACCCGCGAAGGTCAGCCAGTTGTG  >  NC_000913/428336‑428588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑