Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,472	A→G	intergenic (+39/+177)	mutT → / ← yacG	8‑oxo‑dGTP diphosphatase/DNA gyrase inhibitor YacG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,472	0	A	G	100.0%	114.8 / NA	34	intergenic (+39/+177)	mutT/yacG	8‑oxo‑dGTP diphosphatase/DNA gyrase inhibitor YacG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

GGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTTGCACAAGATGCC  >  NC_000913/111339‑111595                                                                                                                                      |                                                                                                                            ggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGc                                                                                                                       >  1:323874/1‑140 (MQ=255) ggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGc                                                                                                                       >  2:30334/1‑140 (MQ=255)  gCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGcc                                                                                                                      >  1:369412/1‑140 (MQ=255)   cAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCt                                                                                                                     <  1:30334/140‑1 (MQ=255)     aCCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGa                                                                                                                   >  2:717424/1‑140 (MQ=255)                ggATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTgg                                                                                                        <  1:32288/140‑1 (MQ=255)                ggATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTgg                                                                                                        <  1:362814/140‑1 (MQ=255)                     tCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGt                                                                                                   <  1:672563/140‑1 (MQ=255)                       gCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCt                                                                                                 >  2:310903/1‑140 (MQ=255)                         tGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTa                                                                                               >  1:593211/1‑140 (MQ=255)                          ggtcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                              <  1:246903/140‑1 (MQ=255)                          ggtcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                              <  1:659405/140‑1 (MQ=255)                                tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                        >  1:328903/1‑140 (MQ=255)                                   tAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaa                                                                                     <  2:695084/140‑1 (MQ=255)                                     aTGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAg                                                                                   <  2:17092/140‑1 (MQ=255)                                       gCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                                                                 <  1:15710/140‑1 (MQ=255)                                       gCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                                                                 <  2:323874/140‑1 (MQ=255)                                          gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                      <  2:79304/132‑1 (MQ=255)                                          gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                      >  1:79304/1‑132 (MQ=255)                                             gatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCt                                                                           <  2:593211/140‑1 (MQ=255)                                              atTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                                                                 <  1:760559/133‑1 (MQ=255)                                              atTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                                                                 >  2:760559/1‑133 (MQ=255)                                                 ttCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACt                                                                       <  2:470421/140‑1 (MQ=255)                                                    cGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCAt                                                                    >  2:78953/1‑140 (MQ=255)                                                         gccaATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATa                                                               <  2:328903/140‑1 (MQ=255)                                                         gccaATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATa                                                               >  2:681730/1‑140 (MQ=255)                                                                    ggTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGttttt                                                    >  1:674078/1‑140 (MQ=255)                                                                        aTTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAg                                                <  2:760073/140‑1 (MQ=255)                                                                        aTTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAg                                                >  1:760073/1‑140 (MQ=255)                                                                         ttGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAgg                                               <  2:674078/140‑1 (MQ=255)                                                                          tGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGt                                              <  1:717424/140‑1 (MQ=255)                                                                              aaGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGa                                          >  2:662182/1‑140 (MQ=255)                                                                                           tGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAAGGCGTTCAcg                             >  2:483438/1‑140 (MQ=255)                                                                                                      aaaGGCGTTTTCGCCGCATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                  <  2:424292/139‑1 (MQ=255)                                                                                                                      cATCCGACATTCGCACGCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTTGCACAAGATGcc  <  2:517888/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                            GGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTTGCACAAGATGCC  >  NC_000913/111339‑111595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑