Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	503,187	C→T	A18T (GCC→ACC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	503,187	0	C	T	100.0%	140.2 / NA	42	A18T (GCC→ACC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

AGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAGTTTATGACAATTTCA  >  NC_000913/503060‑503319                                                                                                                                |                                                                                                                                     aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGgcc                                                                                                                          <  1:17674/140‑1 (MQ=255) aGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGgcc                                                                                                                          <  2:650155/140‑1 (MQ=255)   ttCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGgccgc                                                                                                                        >  1:763728/1‑140 (MQ=255)           cAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAAtg                                                                                                                <  2:502376/140‑1 (MQ=255)             aGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAAtggt                                                                                                              >  2:696151/1‑140 (MQ=255)             aGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAAtggt                                                                                                              <  1:696151/140‑1 (MQ=255)                        ggCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCg                                                                                                   >  2:281365/1‑140 (MQ=255)                         gCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCgg                                                                                                  >  1:27379/1‑140 (MQ=255)                          caacaaAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCggg                                                                                                 >  1:13290/1‑140 (MQ=255)                          caacaaAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCggg                                                                                                 <  1:566923/140‑1 (MQ=255)                           aacaaAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCgggg                                                                                                <  1:501486/140‑1 (MQ=255)                           aacaaAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCgggg                                                                                                <  1:306512/140‑1 (MQ=255)                               aaGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGc                                                                                            <  2:486241/140‑1 (MQ=255)                                  cccGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTg                                                                                         >  2:445263/1‑140 (MQ=255)                                               gTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGtct                                                                            <  1:445263/140‑1 (MQ=255)                                               gTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGtct                                                                            <  1:281365/140‑1 (MQ=255)                                                      cAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTc                                                                     <  2:763728/140‑1 (MQ=255)                                                         cacaCCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCtg                                                                  >  1:57923/1‑140 (MQ=255)                                                                 cTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTAt                                                          >  2:18634/1‑140 (MQ=255)                                                                   aaCAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTg                                                        <  2:27379/140‑1 (MQ=255)                                                                        ataccccACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTc                                                                     >  1:393449/5‑122 (MQ=255)                                                                        atacCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTc                                                                     <  2:393449/122‑1 (MQ=255)                                                                                    aggagAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGa                                       >  1:107778/1‑140 (MQ=255)                                                                                     ggagAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaa                                      <  2:107778/140‑1 (MQ=255)                                                                                      gagaAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGaaa                                     >  1:614462/1‑140 (MQ=255)                                                                                                tAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAAtt                            <  2:534652/139‑1 (MQ=255)                                                                                                tAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAAtt                            >  1:534652/1‑139 (MQ=255)                                                                                                tAGTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTa                           >  2:417024/1‑140 (MQ=255)                                                                                                  gTTTATTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACa                         <  2:8410/140‑1 (MQ=255)                                                                                                      aTTGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTa                     >  1:537319/1‑140 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATTGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaaaa                              >  1:165331/1‑130 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATTGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCaaaa                              <  2:165331/130‑1 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    <  1:498945/140‑1 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    <  1:417024/140‑1 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    <  1:244530/140‑1 (MQ=255)                                                                                                       ttGGCCAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    <  2:715227/140‑1 (MQ=255)                                                                                                           ccAGCATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAGt                >  2:327196/1‑140 (MQ=255)                                                                                                               cATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    <  1:611751/132‑1 (MQ=255)                                                                                                               cATGCCGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTaa                    >  2:611751/1‑132 (MQ=255)                                                                                                                    cGAGGATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAGATTACAGGTAATAGTTTTTGACaa       >  2:744561/1‑140 (MQ=255)                                                                                                                        gATAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCTGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAGTTTATGACAATTTc   >  2:596910/1‑140 (MQ=255)                                                                                                                         aTAAAGGTGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAGTTTATGACAATTTCa  <  2:57923/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     AGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCGAGCACAAGGCCGCCAACAATGGTGGTGATAAGCGGGGTGGCGTGATGCATTCCGTCTCCTTTTCCTGGTGGTTATTGTCCATTTTTGGCCGGGAAAACCAAAATTACAGGTAATAGTTTATGACAATTTCA  >  NC_000913/503060‑503319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑