Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	609,518	A→G	F188L (TTT→CTT)	entD ←	phosphopantetheinyl transferase EntD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	609,518	0	A	G	100.0%	176.0 / NA	50	F188L (TTT→CTT)	entD	phosphopantetheinyl transferase EntD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/28);  total (22/28)

GGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAACATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCGGAAAATGCCAGTGTCAGCG  >  NC_000913/609386‑609652                                                                                                                                     |                                                                                                                                       ggTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCa                                                                                                                                 <  1:653372/140‑1 (MQ=255)     aGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCAt                                                                                                                                     >  2:649091/1‑132 (MQ=255)     aGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCAt                                                                                                                                     <  1:649091/132‑1 (MQ=255)        cACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCAtt                                                                                                                               <  2:86231/135‑1 (MQ=255)        cACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCAtt                                                                                                                               >  1:86231/1‑135 (MQ=255)         aCCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACg                                                                                                                         <  2:632397/140‑1 (MQ=255)           cTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGat                                                                                                                       <  2:773205/140‑1 (MQ=255)                      tttCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGAcc                                                                                                            >  2:379765/1‑140 (MQ=255)                        tCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATtctc                                                                                                                            >  2:440690/1‑122 (MQ=255)                        tCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATtctc                                                                                                                            <  1:440690/122‑1 (MQ=255)                        tCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACctg                                                                                                          >  2:553630/1‑140 (MQ=255)                        tCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACctg                                                                                                          <  2:549398/140‑1 (MQ=255)                        tCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACctg                                                                                                          >  1:812962/1‑140 (MQ=255)                                   cAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTcc                                                                                               <  1:108748/140‑1 (MQ=255)                                    aGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCa                                                                                              >  2:329406/1‑140 (MQ=255)                                      tGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTc                                                                                                >  2:637993/1‑136 (MQ=255)                                      tGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTc                                                                                                <  1:637993/136‑1 (MQ=255)                                       gATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCt                                                                                           <  1:329406/140‑1 (MQ=255)                                       gATGACTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCt                                                                                           <  1:701740/140‑1 (MQ=255)                                           gCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAAtt                                                                                       >  2:638344/1‑140 (MQ=255)                                            cTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTa                                                                                      >  2:423321/1‑140 (MQ=255)                                                 ggCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGa                                                                                 <  1:638344/140‑1 (MQ=255)                                                 ggCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGa                                                                                 <  1:126014/140‑1 (MQ=255)                                                 ggCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGa                                                                                 >  1:308530/1‑140 (MQ=255)                                                 ggCGAATCGTACCAGATGTTGACAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTACCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGa                                                                                 <  1:703512/140‑1 (MQ=255)                                                     aaTCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAg                                                                                             >  2:469716/1‑124 (MQ=255)                                                     aaTCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAg                                                                                             <  1:469716/124‑1 (MQ=255)                                                          tACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGa                                                                        <  1:792958/140‑1 (MQ=255)                                                               gATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAAcc                                                                   >  1:384579/1‑140 (MQ=255)                                                                aTGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCt                                                                  >  1:43297/1‑140 (MQ=255)                                                                  gttgtCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGc                                                                <  1:319048/140‑1 (MQ=255)                                                                         aTTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGtt                                                         <  1:251659/140‑1 (MQ=255)                                                                                  gtTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCa                                                >  1:103113/1‑140 (MQ=255)                                                                                   ttGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCAc                                               <  2:812962/140‑1 (MQ=255)                                                                                    tGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACt                                              <  2:228919/140‑1 (MQ=255)                                                                                    tGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACt                                              <  2:43297/140‑1 (MQ=255)                                                                                    tGGCACAGCGTTATCACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACt                                              <  2:541841/140‑1 (MQ=255)                                                                                          aGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCtt                                        >  1:618907/1‑140 (MQ=255)                                                                                           gCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTa                                       >  2:444122/1‑140 (MQ=255)                                                                                            cGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTaa                                      >  2:29140/1‑140 (MQ=255)                                                                                            cGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTaa                                      >  1:9476/1‑140 (MQ=255)                                                                                            cGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTaa                                      >  1:412885/1‑140 (MQ=255)                                                                                              ttATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAAt                                    <  1:553630/140‑1 (MQ=255)                                                                                                 tGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATgcg                                 <  2:618907/140‑1 (MQ=255)                                                                                                 tGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATgcg                                 <  2:9476/140‑1 (MQ=255)                                                                                                      aTCTTTTCTTTTATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGctctc                            >  1:582333/1‑140 (MQ=255)                                                                                                             cttttATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCg                     <  2:55675/140‑1 (MQ=255)                                                                                                              ttttATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCgg                    >  1:264426/1‑140 (MQ=255)                                                                                                               tttATCTGCCAGTGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCGGa                   <  2:103113/140‑1 (MQ=255)                                                                                                                           tGCACAGCAAGCATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCGGAAAATGCCAgtgt       <  1:250116/140‑1 (MQ=255)                                                                                                                                agcaagcaTCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCGGAAAATGCCAGTGTCAgcg  >  1:304804/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCAGTGATGGCTGCGGGCGAATCGTACCAGATGTTGTCAATTAATCGTGTTGGCACAGCGTTATGACTATCTTTTCTTTTATCTGCCAGTGCACAGCAAACATCTCATTCTCACGATGAATGATGACCTGCTGTTTATTCCAGCTAATTATCTGATAGTCCAGAAAACCTGCATCAGTTTGGATCTCACTTGCCTTAAATGCGCTCTCTTTGGCGGAAAATGCCAGTGTCAGCG  >  NC_000913/609386‑609652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑