Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	134,887	T→C	K232K (AAA→AAG)	speD ←	S‑adenosylmethionine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	134,887	0	T	C	100.0%	257.7 / NA	73	K232K (AAA→AAG)	speD	S‑adenosylmethionine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/42);  total (31/42)

TAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAGACATAAAGTTCTGAAT  >  NC_000913/134752‑135021                                                                                                                                        |                                                                                                                                       taaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTgg                                                                                                                                    >  1:733476/1‑140 (MQ=255)  aaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGgt                                                                                                                                   <  1:543828/140‑1 (MQ=255)  aaatCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGgt                                                                                                                                   <  1:271809/140‑1 (MQ=255)    atCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGgtgt                                                                                                                                 <  1:91706/140‑1 (MQ=255)       tGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGa                                                                                                                              <  2:746771/140‑1 (MQ=255)         gCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAAc                                                                                                                            >  2:816212/1‑140 (MQ=255)         gCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATCTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAAc                                                                                                                            <  2:331770/140‑1 (MQ=255)              gCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTa                                                                                                                       <  1:756087/140‑1 (MQ=255)                     aGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTa                                                                                                                <  2:800531/140‑1 (MQ=255)                       cTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAg                                                                                                              >  2:22748/1‑140 (MQ=255)                         ccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGa                                                                                                                              <  2:5597/122‑1 (MQ=255)                         ccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGa                                                                                                                              >  1:5597/1‑122 (MQ=255)                         ccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGt                                                                                                            >  1:279389/1‑140 (MQ=255)                         ccGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGt                                                                                                            >  2:309822/1‑140 (MQ=255)                           gCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCg                                                                                                          <  1:244882/140‑1 (MQ=255)                            ccAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGa                                                                                                         <  2:706571/140‑1 (MQ=255)                             cAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa                                                                                                        <  2:513333/140‑1 (MQ=255)                               gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTa                                                                                                                >  1:527366/1‑130 (MQ=255)                               gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTa                                                                                                                <  2:527366/130‑1 (MQ=255)                               gagCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAAct                                                                                                      >  2:220755/1‑140 (MQ=255)                                 gCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAActct                                                                                                    <  1:226664/140‑1 (MQ=255)                                      tAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAg                                                                                               <  2:420889/140‑1 (MQ=255)                                       aaaCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGc                                                                                              <  1:22748/140‑1 (MQ=255)                                       aaaCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGc                                                                                              <  2:680115/140‑1 (MQ=255)                                         aCAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCaa                                                                                            <  1:220755/140‑1 (MQ=255)                                          cAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAg                                                                                               >  1:383215/1‑136 (MQ=255)                                          cAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAg                                                                                               <  2:383215/136‑1 (MQ=255)                                               ggCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa                                                                                                        <  1:677735/122‑1 (MQ=255)                                               ggCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGaa                                                                                                        >  2:677735/1‑122 (MQ=255)                                                         gcCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAg                                                                            >  1:462593/1‑140 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   <  2:473683/132‑1 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   >  1:473683/1‑132 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   <  1:520530/132‑1 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   >  2:520530/1‑132 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGa                                                                              >  2:39886/1‑137 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGa                                                                              <  1:39886/137‑1 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGa                                                                           >  1:187319/1‑140 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGa                                                                           >  2:207023/1‑140 (MQ=255)                                                          cccGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGa                                                                           >  1:248560/1‑140 (MQ=255)                                                              taataaATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   >  1:798585/1‑128 (MQ=255)                                                              taataaATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGt                                                                                   <  2:798585/128‑1 (MQ=255)                                                              taataaATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATAtt                                                                       >  2:100986/1‑140 (MQ=255)                                                                   aaTCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCt                                                                  <  1:497231/140‑1 (MQ=255)                                                                     tctcGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGa                                                                >  1:423971/1‑140 (MQ=255)                                                                       tcGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATa                                                              >  2:821602/1‑140 (MQ=255)                                                                        cgcgCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAg                                                             >  1:30672/1‑140 (MQ=255)                                                                         gcgcATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGa                                                            >  2:367884/1‑140 (MQ=255)                                                                         gcgcATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGa                                                            >  1:94952/1‑140 (MQ=255)                                                                           gcATTTCTTTCCACAGCGCAGCGGTAATTTTCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACg                                                          <  2:509037/140‑1 (MQ=255)                                                                           gcATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACg                                                          <  2:462593/140‑1 (MQ=255)                                                                             aTTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGtt                                                        <  2:248560/140‑1 (MQ=255)                                                                             aTTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGtt                                                        <  1:821602/140‑1 (MQ=255)                                                                             aTTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGtt                                                        <  2:715683/140‑1 (MQ=255)                                                                                   ttcCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATc                                                  <  2:728991/140‑1 (MQ=255)                                                                                     ccACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                <  1:614118/140‑1 (MQ=255)                                                                                     ccACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                <  1:367884/140‑1 (MQ=255)                                                                                     ccACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                <  2:733476/140‑1 (MQ=255)                                                                                     ccACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                <  2:30672/140‑1 (MQ=255)                                                                                       acaGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATccacc                                              >  2:439989/1‑140 (MQ=255)                                                                                           cgcagcgGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                <  1:440073/134‑1 (MQ=255)                                                                                           cgcagcgGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATcca                                                >  2:440073/1‑134 (MQ=255)                                                                                           cgcagcgGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGt                                          >  2:602862/1‑140 (MQ=255)                                                                                           cgcagcgGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGt                                          >  1:42499/1‑140 (MQ=255)                                                                                                 ggTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACa                                    <  2:279389/140‑1 (MQ=255)                                                                                                  gTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAg                                   <  2:341795/140‑1 (MQ=255)                                                                                                  gTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAg                                   <  1:602862/140‑1 (MQ=255)                                                                                                          cTGGCGCTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCa                           >  1:6996/1‑140 (MQ=255)                                                                                                             gcgcTCGCTGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCatat                        >  1:604970/1‑140 (MQ=255)                                                                                                                ctcgctGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGt                     >  2:552625/1‑140 (MQ=255)                                                                                                                   gctGTCGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAg                  <  1:207023/140‑1 (MQ=255)                                                                                                                        cGGTTAAGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAGACATa             <  1:309822/140‑1 (MQ=255)                                                                                                                             aaGTCTTCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAGACATAAAGtt        <  1:816212/140‑1 (MQ=255)                                                                                                                                   tCCGGCTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAGACATAAAGTTCTGAAt  <  2:604970/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TAAATCTGGCGGAGCCTGGGAGCTCCGCCAGAGCCGTTAAACAGCTGGCATATTGCGCCCGTAATAAATCTCGCGCATTTCTTTCCACAGCGCAGCGGTAATTTCCTGGCGCTCGCTGTCGGTTAAGTCTTCCGGTTTGGTGTGGAACATGTAGTGCTTAAGGTCGAACTCTTTAAGCAACATCTTGGTATGGAAGATATTTTCCTGATAGACGTTCACATCCACCATGTCATACAGCGCCTTCATATCGTCAGACATAAAGTTCTGAAT  >  NC_000913/134752‑135021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑