| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 634,722 | A→G | A379A (GCA→GCG) | ybdL → | methionine‑oxo‑acid transaminase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 634,722 | 0 | A | G | 92.9% | 78.4 / ‑1.7 | 28 | A379A (GCA→GCG) | ybdL | methionine‑oxo‑acid transaminase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (13/13); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
TGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGC > NC_000913/634587‑634856 | tgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCtt > 2:480128/1‑139 (MQ=255)tgTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTg > 1:533825/1‑140 (MQ=255) aGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGc > 1:310996/1‑140 (MQ=255) aGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGc > 2:282957/1‑140 (MQ=255) gTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGcc > 2:248057/1‑140 (MQ=255) gCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTgcgc < 2:76644/140‑1 (MQ=255) gCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTgcgc < 1:454325/140‑1 (MQ=255) tGGCTGACCCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCt < 2:210614/140‑1 (MQ=255) gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCttt < 1:282957/140‑1 (MQ=255) gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCttt < 2:451874/140‑1 (MQ=255) gCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCttt < 2:746532/140‑1 (MQ=255) tGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAg > 2:56810/1‑140 (MQ=255) aCGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGt > 1:716381/1‑140 (MQ=255) aCGGCGTAGCGGCGATTCCGCTGTCGGTGGTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGt > 2:199386/1‑140 (MQ=255) cGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTc > 1:105095/1‑140 (MQ=255) tAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGgc > 2:17285/1‑140 (MQ=255) aGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGgcg < 2:698353/140‑1 (MQ=255) cggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGcgcg < 1:56810/140‑1 (MQ=255) ggcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCgg < 2:310996/140‑1 (MQ=255) cTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGAc > 1:93368/1‑140 (MQ=255) cGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTc < 1:541633/140‑1 (MQ=255) ggTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTACGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCg < 1:289728/140‑1 (MQ=255) tgtTTTGCGCCGATCCCTTCCCACAT‑AACTGATGCGGCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTAGTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCa < 2:304039/140‑1 (MQ=255) gcgcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaacaa < 2:533825/140‑1 (MQ=255) ttCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGc > 1:727696/1‑140 (MQ=255) aTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAAt > 2:98564/1‑140 (MQ=255) ccAAGAAGGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTtcgtc > 2:471774/1‑140 (MQ=255) aagaagGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGc < 2:716381/140‑1 (MQ=255) agaagGAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCt > 2:108883/1‑140 (MQ=255) ggAATCGACGTTGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTcc < 1:17285/140‑1 (MQ=255) tGCTGGCAGCGGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGctc > 2:664278/1‑140 (MQ=255) ggCAGCGGCTGAACGCCTGCGCAAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCTGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCGCTTTg < 2:727696/140‑1 (MQ=255) gcagcgGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGc < 2:301079/140‑1 (MQ=255) gcagcgGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGc < 1:772882/140‑1 (MQ=255) gcagcgGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGc < 1:108883/140‑1 (MQ=255) | TGTTGAGTTTTGCCAGTGGCTGACGCAGGAGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGC > NC_000913/634587‑634856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |