| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 646,209 | A→G | R124R (CGT→CGC) | citT ← | citrate:succinate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,209 | 0 | A | G | 94.7% | 115.5 / ‑1.7 | 38 | R124R (CGT→CGC) | citT | citrate:succinate antiporter |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (17/19); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
ACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAAT > NC_000913/646072‑646344 | aCCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATgcggcg > 1:74350/1‑140 (MQ=255) ccGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGcggcga > 1:79785/1‑140 (MQ=255) gTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGa < 2:358896/140‑1 (MQ=255) gTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGa < 1:725748/140‑1 (MQ=255) gTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGa < 1:714901/140‑1 (MQ=255) aCCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGaaa < 1:193152/140‑1 (MQ=255) cGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCa < 1:470489/140‑1 (MQ=255) ggTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCAt > 2:19379/1‑140 (MQ=255) gTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATa > 1:529952/1‑140 (MQ=255) tgtTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCa > 2:659295/1‑140 (MQ=255) tgtAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATg > 1:404892/1‑140 (MQ=255) aaCGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGcc < 1:16671/140‑1 (MQ=255) aaCGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGcc < 2:74350/140‑1 (MQ=255) aaCGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGcc < 1:472356/140‑1 (MQ=255) tGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATa < 2:79785/140‑1 (MQ=255) gCCAGCAGAATGTCGATAATGATAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATAc < 1:587492/140‑1 (MQ=255) gCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATAc > 1:105049/1‑140 (MQ=255) agcagAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAg < 2:529952/140‑1 (MQ=255) cagAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGcc < 1:19379/140‑1 (MQ=255) gTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCg > 1:229881/1‑140 (MQ=255) tAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCgtggtg > 1:772747/1‑140 (MQ=255) tAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCgtggtg < 2:404892/140‑1 (MQ=255) tAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCgtggtg > 1:437504/1‑140 (MQ=255) aTGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCt > 2:799554/1‑140 (MQ=255) tCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGaaaaa < 2:772747/140‑1 (MQ=255) tCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGaaaaa < 2:437504/140‑1 (MQ=255) tAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAAccagc > 1:597838/1‑140 (MQ=255) aaCGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCAATACCGTGGTGCTGGAAAAACCAGCCAGGcc > 1:90962/1‑140 (MQ=255) aCGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGccc > 2:566658/1‑140 (MQ=255) gcgtgcgtTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTc > 1:177998/1‑140 (MQ=255) cgtgcgtTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAAccagcca < 1:304194/128‑1 (MQ=255) cgtgcgtTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAAccagcca > 2:304194/1‑128 (MQ=255) gCCCATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGtt > 2:793702/1‑140 (MQ=255) cATGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGttttt < 1:8804/140‑1 (MQ=255) tGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTgc < 2:597838/140‑1 (MQ=255) tGAATTTCACCAGGAAAAGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTgc > 1:815625/1‑140 (MQ=255) aaaGGGCAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTggg < 2:429838/140‑1 (MQ=255) cAATGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCt < 1:566658/140‑1 (MQ=255) tGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAAt > 2:410306/1‑140 (MQ=255) tGCGGCGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAAt > 1:473420/1‑140 (MQ=255) | ACCGGAAAAACCGTACCCCCGGTACGCGCGGTGTTGGAAGGTGTAAACGGTGCCAGCAGAATGTCGATAATGACAATCGCATAACCCAACGTCAGCGTGCGTTTGCCCATGAATTTCACCAGGAAAAGGGCAATGCGACGACCTAACCCGGAAACTTCATACCCTAATGCAAAAATAAATGCGCCAAATACCAGCCATACCGTGGTGCTGGAAAAACCAGCCAGGCCCCATTTCAGCGCCTGTTTTTGCGCATTAAACGCTGGGTCAGCTAAT > NC_000913/646072‑646344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |