Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,763	G→A	A86T (GCA→ACA)	ybeU →	DUF1266 domain‑containing protein YbeU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,763	0	G	A	100.0%	179.7 / NA	52	A86T (GCA→ACA)	ybeU	DUF1266 domain‑containing protein YbeU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/28);  total (24/28)

TCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGT  >  NC_000913/679629‑679896                                                                                                                                       |                                                                                                                                      tctcATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCATATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTa                                                                                                                                  <  1:384223/140‑1 (MQ=255)        atcCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCtg                                                                                                                           <  1:390495/140‑1 (MQ=255)          ccTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCtggt                                                                                                                         <  1:130529/140‑1 (MQ=255)             ggggAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGc                                                                                                                      >  2:465504/1‑140 (MQ=255)                 aataaataATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCgg                                                                                                                  >  2:769798/1‑140 (MQ=255)                    aaataaTCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGgaga                                                                                                               <  1:720366/140‑1 (MQ=255)                    aaataaTCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGgaga                                                                                                               <  2:262736/140‑1 (MQ=255)                          tCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGt                                                                                                         <  2:645483/140‑1 (MQ=255)                          tCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCACTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGt                                                                                                         >  1:623800/1‑140 (MQ=255)                                  atTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTtat                                                                                                 <  2:746805/140‑1 (MQ=255)                                            gACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCg                                                                                       >  2:673711/1‑140 (MQ=255)                                              cTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGtt                                                                                     <  1:465504/140‑1 (MQ=255)                                                       aGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAAcc                                                                            <  2:412515/140‑1 (MQ=255)                                                         aTGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGc                                                                          >  1:405363/1‑140 (MQ=255)                                                         aTGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGc                                                                          >  2:213653/1‑140 (MQ=255)                                                           gACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGc                                                                                   >  2:752569/1‑129 (MQ=255)                                                           gACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGc                                                                                   <  1:752569/129‑1 (MQ=255)                                                           gACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTa                                                                        >  1:393332/1‑140 (MQ=255)                                                           gACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTa                                                                        >  1:384935/1‑140 (MQ=255)                                                             cTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTAtg                                                                      <  2:393332/140‑1 (MQ=255)                                                               gatgatGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTAtgtg                                                                    <  1:366524/140‑1 (MQ=255)                                                                     ggTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTgg                                                              >  1:664261/1‑140 (MQ=255)                                                                          tGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCtgt                                                                <  1:583825/133‑1 (MQ=255)                                                                          tGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCtgt                                                                >  2:583825/1‑133 (MQ=255)                                                                          tGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAg                                                         >  1:31134/1‑140 (MQ=255)                                                                                tGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGaa                                                   >  2:816360/1‑140 (MQ=255)                                                                                     tAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCaaa                                              <  2:664261/140‑1 (MQ=255)                                                                                      aGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCa                                                <  2:15414/137‑1 (MQ=255)                                                                                      aGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCa                                                >  1:15414/1‑137 (MQ=255)                                                                                       gCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGc                                            <  1:696128/140‑1 (MQ=255)                                                                                       gCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGc                                            <  1:673711/140‑1 (MQ=255)                                                                                         tGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCt                                          <  1:495831/140‑1 (MQ=255)                                                                                            cTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTggg                                       >  2:545757/1‑140 (MQ=255)                                                                                             ttATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGa                                      <  2:31134/140‑1 (MQ=255)                                                                                                     gACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGa                                      >  2:825487/1‑132 (MQ=255)                                                                                                     gACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGa                                      <  1:825487/132‑1 (MQ=255)                                                                                                     gACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTc                              >  2:722677/1‑140 (MQ=255)                                                                                                        aTGGCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGaa                           <  1:545757/140‑1 (MQ=255)                                                                                                          ggCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATg                         >  1:282567/1‑140 (MQ=255)                                                                                                          ggCATCGCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATg                         >  1:553263/1‑140 (MQ=255)                                                                                                                gCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGttt                   <  2:282567/140‑1 (MQ=255)                                                                                                                gCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGttt                   <  1:213653/140‑1 (MQ=255)                                                                                                                gCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGttt                   <  1:816360/140‑1 (MQ=255)                                                                                                                gCTCTTCCCCTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGttt                   <  2:553263/140‑1 (MQ=255)                                                                                                                      ccccTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCGAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCa             >  2:574166/1‑140 (MQ=255)                                                                                                                      ccccTGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCa             >  1:502023/1‑140 (MQ=255)                                                                                                                          tGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACggg         <  1:722677/140‑1 (MQ=255)                                                                                                                          tGAAGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACggg         <  2:405363/140‑1 (MQ=255)                                                                                                                            aaGAGTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTc       >  1:233626/1‑140 (MQ=255)                                                                                                                                gTGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGgtg   <  2:502023/140‑1 (MQ=255)                                                                                                                                 tGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTGTGTTCGAATGGGGTTTCTGTCACGGGTCGgtgt  >  2:537115/1‑140 (MQ=255)                                                                                                                                 tGGAAAACATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGgtgt  >  1:386686/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      TCTCATCATCCTGGGGAATAAATAATCGTGATGATTTAATTCAGACTATTTATCAGATGACTGATGATGGTCATGCCAATGATTTAGCTGGCTTATATCTGACATGGCATCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGT  >  NC_000913/679629‑679896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑