Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,870	T→C	S27S (AGT→AGC)	chiQ →	lipoprotein ChiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,870	0	T	C	100.0%	134.8 / NA	39	S27S (AGT→AGC)	chiQ	lipoprotein ChiQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/23);  total (16/23)

CTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTAC  >  NC_000913/709734‑710006                                                                                                                                         |                                                                                                                                         cttctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAgccgt                                                                                                                                       >  2:179854/1‑140 (MQ=255)   tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCt                                                                                                                                     >  2:138650/1‑140 (MQ=255)   tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCt                                                                                                                                     >  1:696127/1‑140 (MQ=255)     tGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGa                                                                                                                                   <  1:169995/140‑1 (MQ=255)         gCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAaga                                                                                                                               <  1:692986/140‑1 (MQ=255)             gACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCa                                                                                                                           <  2:780977/140‑1 (MQ=255)                  ggCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAg                                                                                                                      <  1:516826/140‑1 (MQ=255)                   gCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAgc                                                                                                                     >  1:391064/1‑140 (MQ=255)                      ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  <  2:391064/140‑1 (MQ=255)                      ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  <  2:354056/140‑1 (MQ=255)                      ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  <  1:458236/140‑1 (MQ=255)                      ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  <  1:532965/140‑1 (MQ=255)                            gtgCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATc                                                                                                            >  1:679321/1‑140 (MQ=255)                                ctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAaca                                                                                                        >  1:715527/1‑140 (MQ=255)                                 tgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  <  1:692130/129‑1 (MQ=255)                                 tgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGcc                                                                                                                  >  2:692130/1‑129 (MQ=255)                                                   tttGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGa                                                                                     >  1:572808/1‑140 (MQ=255)                                                     tGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGaaa                                                                                   >  2:47304/1‑140 (MQ=255)                                                     tGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGaaa                                                                                   >  1:189723/1‑140 (MQ=255)                                                     tGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGaaa                                                                                   >  1:274453/1‑140 (MQ=255)                                                             aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                           <  2:101088/140‑1 (MQ=255)                                                             aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                           <  2:274453/140‑1 (MQ=255)                                                             aaaaaCTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGaa                                                                           <  2:28352/140‑1 (MQ=255)                                                                   tGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGc                                                                     <  2:696127/140‑1 (MQ=255)                                                                       tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                                 <  2:431491/140‑1 (MQ=255)                                                                        ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                                <  2:189723/140‑1 (MQ=255)                                                                        ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                                <  2:715527/140‑1 (MQ=255)                                                                        ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                                <  1:548506/140‑1 (MQ=255)                                                                                   aaTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                                 <  2:321472/128‑1 (MQ=255)                                                                                   aaTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                                 >  1:321472/1‑128 (MQ=255)                                                                                    aTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGt                                                    <  2:572808/140‑1 (MQ=255)                                                                                     tGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTg                                                   >  2:247642/1‑140 (MQ=255)                                                                                             gggCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaagaa                                           >  2:794194/1‑140 (MQ=255)                                                                                                  ggTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAgaga                                      >  1:635971/1‑140 (MQ=255)                                                                                                           tgCGCAATCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATc                             >  1:265272/1‑140 (MQ=255)                                                                                                                  tCAACCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGtt                      >  1:59780/1‑140 (MQ=255)                                                                                                                    aaCCGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTg                    <  2:679321/140‑1 (MQ=255)                                                                                                                      ccGCGCCACAGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGcc                  <  1:179854/140‑1 (MQ=255)                                                                                                                            cacaGGAAGACAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAg            <  2:738526/140‑1 (MQ=255)                                                                                                                                      cAGCCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTAc  <  1:247642/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTAC  >  NC_000913/709734‑710006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑