Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	731,455	T→C	C625R (TGT→CGT)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	731,455	0	T	C	100.0%	59.3 / NA	20	C625R (TGT→CGT)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/6);  total (14/6)

TAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCTGTACCACGCAGGGCGGTCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTG  >  NC_000913/731324‑731520                                                                                                                                    |                                                                  tAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACg                                                           <  2:109753/140‑1 (MQ=255)        cGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCg                                                    >  2:427192/1‑140 (MQ=255)                  acGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACAGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          <  2:361939/140‑1 (MQ=255)                    gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          <  1:247255/138‑1 (MQ=255)                    gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          >  2:5982/1‑138 (MQ=255)                    gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          <  1:5982/138‑1 (MQ=255)                    gCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          >  2:247255/1‑138 (MQ=255)                         gCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATg                                   >  2:402388/1‑140 (MQ=255)                                  cgcgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          >  2:772443/1‑124 (MQ=255)                                  cgcgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGAcgcg                                          <  1:772443/124‑1 (MQ=255)                                  cgcgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGAt                          >  2:666633/1‑140 (MQ=255)                                    cgGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGAtgc                        >  1:178937/1‑140 (MQ=255)                                                 aCGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTc           >  2:113862/1‑140 (MQ=255)                                                 aCGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTc           >  1:355730/1‑140 (MQ=255)                                                     cgccgGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATcc       >  2:213203/1‑140 (MQ=18)                                                     cgccgGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATcc       >  1:561794/1‑140 (MQ=18)                                                     cgccgGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATcc       >  2:622732/1‑140 (MQ=17)                                                          gTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTg  >  1:751322/1‑140 (MQ=18)                                                                  accaccGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGcc    <  2:421935/130‑1 (MQ=17)                                                                  accaccGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCCGTACCACGCAGGGCGGGCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGcc    >  1:421935/1‑130 (MQ=18)                                                                                                                                    |                                                                  TAATTGCCGTGAAAGACACGCAGGGCCATGAAACGCGGTATGAATACAACGCCGCCGGTGACCTGACCACCGTCATTGCCCCGGACGGCAGCAGAAACGGGACACAGTACGATGCGTGGGGAAAAGCCATCTGTACCACGCAGGGCGGTCTGACGCGCAGTATGGAATACGATGCTGCCGGACGGGTCATCCGCCTG  >  NC_000913/731324‑731520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑