Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	810,031	A→G	T230A (ACG→GCG)	bioB →	biotin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	810,031	0	A	G	100.0%	103.2 / NA	31	T230A (ACG→GCG)	bioB	biotin synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

CAAAGTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCACGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAG  >  NC_000913/809898‑810162                                                                                                                                      |                                                                                                                                    cAAAGTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGcgccgc                                                                                                                               <  1:145412/140‑1 (MQ=255)         gTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGAt                                                                                                                       <  1:100995/140‑1 (MQ=255)                cggcATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACgatg                                                                                                                <  1:266355/140‑1 (MQ=255)                       gtgGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGa                                                                                                         <  2:585111/140‑1 (MQ=255)                                  cGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGAtt                                                                                              <  2:770889/140‑1 (MQ=255)                                    aaaCGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGAtt                                                                                              <  1:178811/138‑1 (MQ=255)                                    aaaCGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGAtt                                                                                              >  2:178811/1‑138 (MQ=255)                                      aCGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTAt                                                                                          <  2:222667/140‑1 (MQ=255)                                                  cgcgCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGc                                                                              >  1:75799/1‑140 (MQ=255)                                                    cgcCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCg                                                                                       >  2:796370/1‑129 (MQ=255)                                                    cgcCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCg                                                                                       <  1:796370/129‑1 (MQ=255)                                                       cGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTcg                                                                         <  1:333682/140‑1 (MQ=255)                                                         gATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTcgcg                                                                       <  1:457337/140‑1 (MQ=255)                                                          attattGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTcgcgc                                                                      <  2:75799/140‑1 (MQ=255)                                                                    cAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGt                                                                           <  1:493944/125‑1 (MQ=255)                                                                    cAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGt                                                                           >  2:493944/1‑125 (MQ=255)                                                                      aCTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGc                                                          >  1:171617/1‑140 (MQ=255)                                                                       cTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGcc                                                         >  2:669801/1‑140 (MQ=255)                                                                         ggCAAACCTGTCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGGTAACGTTGCTGTCGATGCCTTTGATTTTATTCGCACCATGGCGGTCGCGCGGATCATGATGCCaa                                                       >  2:183456/1‑140 (MQ=255)                                                                            aaaCCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTG‑AGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACctc                                                   >  1:736799/1‑140 (MQ=255)                                                                                      aCGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTgcg                                          <  2:171617/140‑1 (MQ=255)                                                                                                      tGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGAcgc                          >  2:776159/1‑140 (MQ=255)                                                                                                      tGCCAATCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGAcgc                          >  2:785554/1‑140 (MQ=255)                                                                                                           aTCAACATGCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCa                     <  1:785554/140‑1 (MQ=255)                                                                                                                   gCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATg                 >  1:465755/1‑136 (MQ=255)                                                                                                                   gCTGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATg                 <  2:465755/136‑1 (MQ=255)                                                                                                                     tGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATgaacgaa           >  1:487789/1‑140 (MQ=255)                                                                                                                     tGGTGAAGGTGAAAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATgaacgaa           >  1:674722/1‑140 (MQ=255)                                                                                                                          aaggtgaaAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGAc      >  1:805998/1‑140 (MQ=255)                                                                                                                              tgaaAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAg  >  2:352157/1‑140 (MQ=255)                                                                                                                              tgaaAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAg  >  2:658462/1‑140 (MQ=255)                                                                                                                              tgaaAGGCGCGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAg  <  1:206961/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    CAAAGTCTGTTCTGGCGGCATTGTGGGCTTAGGCGAAACGGTAAAAGATCGCGCCGGATTATTGCTGCAACTGGCAAACCTGCCGACGCCGCCGGAAAGCGTGCCAATCAACATGCTGGTGAAGGTGAAAGGCACGCCGCTTGCCGATAACGATGATGTCGATGCCTTTGATTTTATTCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAG  >  NC_000913/809898‑810162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑