BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	823,294	A→G	V149A (GTA→GCA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	823,294	0	A	G	100.0%	104.6 / NA	33	V149A (GTA→GCA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/18); total (15/18)

CTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCG  >  NC_000913/823169‑823427

ctcCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGt                                                                                                                         >  2:687720/1‑140 (MQ=255)

  cccGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCGGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGttt                                                                                                                       <  2:624200/140‑1 (MQ=255)

  cccGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGttt                                                                                                                       <  2:286798/140‑1 (MQ=255)

          gCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGttt                                                                                                                       >  2:434569/1‑132 (MQ=255)

          gCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGttt                                                                                                                       <  1:434569/132‑1 (MQ=255)

          gCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAg                                                                                                                     >  1:222266/1‑134 (MQ=255)

          gCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAg                                                                                                                     <  2:222266/134‑1 (MQ=255)

                  ctTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAg                                                                                                       <  2:22280/140‑1 (MQ=255)

                     ccGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTg                                                                                                    <  1:687720/140‑1 (MQ=255)

                     ccGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCATAGCTg                                                                                                    <  1:350575/140‑1 (MQ=255)

                        cTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGAc                                                                                                 >  1:402287/1‑140 (MQ=255)

                        cTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGAc                                                                                                 <  1:674309/140‑1 (MQ=255)

                                  aCGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTcgg                                                                                       >  1:623061/1‑140 (MQ=255)

                                            caGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTcgg                                                                                       <  1:708171/130‑1 (MQ=255)

                                            caGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTcgg                                                                                       >  2:708171/1‑130 (MQ=255)

                                            caGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAAtt                                                                             >  2:278032/1‑140 (MQ=255)

                                               cgtcgtGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAg                                                                          >  1:257495/1‑140 (MQ=255)

                                                  cgtGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGccc                                                                       >  2:647037/1‑140 (MQ=255)

                                                   gtGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGGGGAGTAATTCAGCCCg                                                                      <  1:65198/140‑1 (MQ=255)

                                                   gtGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCg                                                                      <  1:647037/140‑1 (MQ=255)

                                                             ctGCCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAgg                                                            <  2:225873/140‑1 (MQ=255)

                                                              tGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATa                                                                >  2:98489/1‑135 (MQ=255)

                                                              tGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATa                                                                <  1:98489/135‑1 (MQ=255)

                                                                  caggcaggTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCtata                                                       <  1:53906/140‑1 (MQ=255)

                                                                        gtttttCCAGCTAAACCTGGAGAATATCTTCGACAATCGGCCCTTCAGGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGAAATATGCTGGGGGGAGTAATTCAGCCCGCCAATAAAGGCTATAcgcgcg                                                 <  2:402287/138‑1 (MQ=255)

                                                                          ttttCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTc                                               >  2:434959/1‑140 (MQ=255)

                                                                                  cTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATcacca                                       <  1:434959/140‑1 (MQ=255)

                                                                                   tCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATcaccac                                      >  1:471474/1‑140 (MQ=255)

                                                                                       aCTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAAtt                                  >  1:160116/1‑140 (MQ=255)

                                                                                                 atCTTCGACAATCGGCCTTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCa                        <  1:735570/140‑1 (MQ=255)

                                                                                                        aCAATCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCg                 <  2:160116/140‑1 (MQ=255)

                                                                                                          aaTCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCgg                   >  2:240132/1‑136 (MQ=255)

                                                                                                          aaTCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCgg                   <  1:240132/136‑1 (MQ=255)

                                                                                                           aTCGGCCCTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGaaa              >  1:8290/1‑140 (MQ=255)

                                                                                                                 ccTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACAtt        <  2:471474/140‑1 (MQ=255)

                                                                                                                 ccTTCAAGGCGTGCCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACAtt        <  2:623061/140‑1 (MQ=255)

                                                                                                                       gggCGTGCCGCGTAATCCTTTTTAGCTTCGGGCCCGTGGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTgcg  <  2:790775/139‑1 (MQ=255)

CTCCCGGCTGGCGGTTCTCTTCCGCTTTGTGATGACGTCGCCACCAGCGTCGTGCCGCGCTCTGTCCAGGCAGGTTTTCCAGCTCAAACTGGAGAATATCTTCGACAATCGGCCCTTCAAGGCGTACCGCGTAATCCTGTTTAGCCTCTGGACCGTAGCTGGACATATGCTCGGCGGAGTAATTCAGCCCGCCAATAAAGGCTATACGCGCGTCGATCACCACAATTTTGCGATGCATCCGGCGAAACACATTGGTGCG  >  NC_000913/823169‑823427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`