Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	902,309	A→G	V227A (GTG→GCG)	artI ←	putative ABC transporter periplasmic binding protein ArtI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	902,309	0	A	G	100.0%	119.0 / NA	35	V227A (GTG→GCG)	artI	putative ABC transporter periplasmic binding protein ArtI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/14);  total (21/14)

CCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCACTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCA  >  NC_000913/902172‑902444                                                                                                                                          |                                                                                                                                        ccagcCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCt                                                                                                                                       >  1:772040/1‑140 (MQ=255)     cccGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCttttt                                                                                                                                   >  1:800628/1‑140 (MQ=255)        gACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCa                                                                                                                                <  2:193745/140‑1 (MQ=255)              caatGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAg                                                                                                                          <  2:772040/140‑1 (MQ=255)              caatGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAg                                                                                                                          <  2:206196/140‑1 (MQ=255)                    acaAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGa                                                                                                                    >  2:498233/1‑140 (MQ=255)                                   gACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCt                                                                                                     <  2:777216/140‑1 (MQ=255)                                    aCGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCt                                                                                                     >  1:145936/1‑139 (MQ=255)                                    aCGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCt                                                                                                     <  2:145936/139‑1 (MQ=255)                                          aTCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGtt                                                                                              >  1:587401/1‑140 (MQ=255)                                           tCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTg                                                                                             <  2:800628/140‑1 (MQ=255)                                               ggcggcGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCt                                                                                         >  2:466211/1‑140 (MQ=255)                                                    cgcTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGTAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGt                                                                                    >  2:778491/1‑140 (MQ=255)                                                    cgcTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGccc                                                                                          >  1:386899/1‑134 (MQ=255)                                                    cgcTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGccc                                                                                          <  2:386899/134‑1 (MQ=255)                                                                    aaaTTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGcc                                                                         <  2:99394/135‑1 (MQ=255)                                                                    aaaTTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGcc                                                                         >  1:99394/1‑135 (MQ=255)                                                                           ttcatCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGcc                                                             >  1:263915/1‑140 (MQ=255)                                                                               tcaGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAg                                                         <  1:263347/140‑1 (MQ=255)                                                                               tcaGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAg                                                         <  2:496416/140‑1 (MQ=255)                                                                               tcaGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAg                                                         >  2:381248/1‑140 (MQ=255)                                                                                                aaCCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCAc                                        >  2:247773/1‑140 (MQ=255)                                                                                                    aTTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTg                                    >  2:98026/1‑140 (MQ=255)                                                                                                     tttGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGt                                   >  1:313894/1‑140 (MQ=255)                                                                                                          tgtAGATGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGccc                              >  1:495645/1‑140 (MQ=255)                                                                                                                tGGTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCAccgcg                        <  1:98026/140‑2 (MQ=255)                                                                                                                  gTTTCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACcgccgc                      <  2:313894/140‑1 (MQ=255)                                                                                                                   tttCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCgccgcc                     >  1:211693/1‑140 (MQ=255)                                                                                                                   tttCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCgccgcc                     >  2:738227/1‑140 (MQ=255)                                                                                                                   tttCGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCgccgcc                     >  1:699832/1‑140 (MQ=255)                                                                                                                      cGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACcgccgc                      <  1:120925/136‑1 (MQ=255)                                                                                                                      cGTAAGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACcgccgc                      >  2:120925/1‑136 (MQ=255)                                                                                                                          aGTGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCg              <  1:53395/140‑1 (MQ=255)                                                                                                                            tGCCATCTTTCTTCGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCggg            >  1:165503/1‑140 (MQ=255)                                                                                                                                      cttcGCTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCa  >  2:738353/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CCAGCCCGACAATCAATGCACAAACGGCAAGGCCGACGGTCATCCCGGCGGCGCTTGCTAAAGGAAAAAATTCATTCATCAGGAATTACTTCTGGAACCATTTGTTGTAGATGGTTTCGTAAGTGCCATCTTTCTTCACTTTTTCCAGCGCAGTGTTGAGTTTCTGCTGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCA  >  NC_000913/902172‑902444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑