Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	925,969	A→G	noncoding (3/88 nt)	serW ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,969	0	A	G	100.0%	192.0 / NA	55	noncoding (3/88 nt)	serW	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

AAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGT  >  NC_000913/925843‑926097                                                                                                                               |                                                                                                                                 aaaaaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTg                                                                                                                     <  1:413050/140‑1 (MQ=38)    aaaaaGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTa                                                                                                                  <  1:41013/140‑1 (MQ=255)       aaGCTCGCACTTTCGTACGAGCTCTTCTTTAAAGATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCTAAATTGTTTTGCTACCa                                                                                                               <  2:560900/140‑1 (MQ=255)              cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCa                                                                                                        <  1:804291/140‑1 (MQ=255)              cACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCa                                                                                                        <  1:779209/140‑1 (MQ=255)                          aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACg                                                                                            <  1:369467/140‑1 (MQ=255)                           gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCCCAACCTTTCCGTATACACACCTTTCCCGCGTGCTCCTTCCCCCCCTCCGGCACCTCCCCCAAATTTTTTTTTTCCAAACCTCATGGGGGGGAACgg                                                                                           >  2:369467/1‑140 (MQ=9)                             tcttctTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGATCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACgggg                                                                                         <  2:531554/140‑1 (MQ=255)                               ttcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcg                                                                                       <  2:52255/140‑1 (MQ=255)                               ttcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcg                                                                                       <  2:270439/140‑1 (MQ=255)                               ttcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcg                                                                                       <  1:54969/140‑1 (MQ=255)                               ttcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcg                                                                                       <  1:271761/140‑1 (MQ=255)                               gtcttTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGgcg                                                                                       <  1:722096/139‑1 (MQ=255)                                       atatGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACtata                                                                               <  1:230569/140‑1 (MQ=255)                                           ggCGGGGAGGGGGGGGGTCGAACCCCCCATACCTTGCCGTATAAACACCTTCCCGGCGGGCTCCCTCCCCCACCCGGACACCCCCCCCAAATGTTTTTTTTCCAAAACTCATTGGGGGGAACGGGGCGCTACTATAgggg                                                                           >  2:250552/1‑139 (MQ=255)                                            gCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAg                                                                          <  1:455915/140‑1 (MQ=255)                                                              gAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCaa                                                        >  2:419898/1‑140 (MQ=255)                                                                   cccGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAagaaga                                                   <  1:76895/140‑1 (MQ=255)                                                                            ttGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATg                                          >  1:769546/1‑140 (MQ=255)                                                                             tGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGa                                         >  2:348884/1‑140 (MQ=255)                                                                                  tataCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAatt                                    >  2:661627/1‑140 (MQ=255)                                                                                  tataCACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAatt                                    >  2:58937/1‑140 (MQ=255)                                                                                       acacTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGGGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGt                               <  1:104578/140‑1 (MQ=255)                                                                                        cacTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGTCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGtt                              <  1:58937/140‑1 (MQ=255)                                                                                        cacTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGtt                              <  1:661627/140‑1 (MQ=255)                                                                                         acTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAGTTTTAATGATAATTATTGttt                             >  2:754826/1‑140 (MQ=255)                                                                                         acTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGttt                             >  2:691431/1‑140 (MQ=255)                                                                                         acTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGttt                             >  2:567759/1‑140 (MQ=255)                                                                                         acTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGttt                             >  2:627740/1‑140 (MQ=255)                                                                                         acTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGttt                             >  1:56919/1‑140 (MQ=255)                                                                                            ttCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCt                          >  2:486040/1‑140 (MQ=255)                                                                                             tCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTc                         <  1:195175/140‑1 (MQ=255)                                                                                              ccAGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCa                        >  2:285353/1‑140 (MQ=255)                                                                                                aGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATa                      <  1:530181/140‑1 (MQ=255)                                                                                                aGGCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATa                      <  1:486040/140‑1 (MQ=255)                                                                                                 ggCGTGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATAc                     <  1:754826/140‑1 (MQ=255)                                                                                                     tGCTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTa                 >  2:38539/1‑140 (MQ=255)                                                                                                       cTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTg                   >  2:156298/1‑136 (MQ=255)                                                                                                       cTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTg                   <  1:156298/136‑1 (MQ=255)                                                                                                       cTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTaaa               >  2:555822/1‑140 (MQ=255)                                                                                                       cTCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTaaa               >  2:25695/1‑140 (MQ=255)                                                                                                        tCCTTCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAAc              <  1:627740/140‑1 (MQ=255)                                                                                                           ttCAGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAg           <  2:56919/140‑1 (MQ=255)                                                                                                              aGCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTtg        >  1:577615/1‑140 (MQ=255)                                                                                                               gCCACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTtgt       <  1:625408/140‑1 (MQ=255)                                                                                                                ccACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTtgtg      <  1:532760/140‑1 (MQ=255)                                                                                                                ccACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATCGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTtgtg      <  2:769546/140‑1 (MQ=255)                                                                                                                 cACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGc     <  1:285353/140‑1 (MQ=255)                                                                                                                 cACTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGc     <  1:156254/140‑1 (MQ=255)                                                                                                                  aCTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACagg           >  2:359551/1‑131 (MQ=255)                                                                                                                  aCTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACagg           <  1:359551/133‑3 (MQ=255)                                                                                                                   cTCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAg   <  1:713279/140‑1 (MQ=255)                                                                                                                    tCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAg   <  2:562085/139‑1 (MQ=255)                                                                                                                    tCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAg   >  1:562085/1‑139 (MQ=255)                                                                                                                    tCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGt  >  2:358840/1‑140 (MQ=255)                                                                                                                    tCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGt  >  1:525964/1‑140 (MQ=255)                                                                                                                    tCGGACACCTCGCCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGt  >  1:455962/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                 AAAAAAAAGCTCGCACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTTTTGCTACCAAACCTCATGGGTGGCAACGGGGCGCTACTATAGGGAGTTGGAGTAAAACGGTCAAGAAGAATTTTAATGATAATTATTGTTTGCTCATACTGTAAACAAGTTGTGCAGT  >  NC_000913/925843‑926097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑