Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,247	T→G	T394P (ACG→CCG)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,247	0	T	G	100.0%	165.3 / NA	48	T394P (ACG→CCG)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/22);  total (26/22)

TTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAAC  >  NC_000913/154115‑154376                                                                                                                                     |                                                                                                                                  ttttATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGATTATCTGAATACCGGTATAACCCGGCAGATAg                                                                                                                            <  2:179258/140‑1 (MQ=255)    tATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCACCGGAATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTa                                                                                                                         >  2:721010/1‑140 (MQ=255)     atcatcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTAt                                                                                                                        >  1:566330/1‑140 (MQ=255)      tcatcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTAtt                                                                                                                       <  2:312517/140‑1 (MQ=255)      tcatcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTAtt                                                                                                                       >  2:114432/1‑140 (MQ=255)        atcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCa                                                                                                                     <  2:321927/140‑1 (MQ=255)         tcCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAg                                                                                                                    >  2:663959/1‑140 (MQ=255)           cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGc                                                                                                                  >  2:583072/1‑140 (MQ=255)           cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGc                                                                                                                  <  1:829682/140‑1 (MQ=255)           cGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGc                                                                                                                  <  1:583072/140‑1 (MQ=255)             ggATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCg                                                                                                                <  1:114432/140‑1 (MQ=255)             ggATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCg                                                                                                                <  1:622797/140‑1 (MQ=255)                tACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCgtag                                                                                                             <  1:285900/140‑1 (MQ=255)                 aCGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCgtagt                                                                                                            <  1:663959/140‑1 (MQ=255)                       aTTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAgc                                                                                                      <  2:566330/140‑1 (MQ=255)                                 gTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaaa                                                                                            >  1:504895/1‑140 (MQ=255)                                 gTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaaa                                                                                            >  1:572450/1‑140 (MQ=255)                                 gTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaa                                                                                             >  2:765374/1‑139 (MQ=255)                                 gTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaa                                                                                             <  1:765374/139‑1 (MQ=255)                                      aTCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaaataaat                                                                                       <  2:618239/140‑1 (MQ=255)                                          aaagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAgg                                                                                   >  1:87251/1‑140 (MQ=255)                                           aagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGTTTGAAATAAATTAGGc                                                                                  <  1:760821/140‑1 (MQ=255)                                           aagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGc                                                                                  <  2:56493/140‑1 (MQ=255)                                           aagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGc                                                                                  <  2:192673/140‑1 (MQ=255)                                           aagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGc                                                                                  <  1:721010/140‑1 (MQ=255)                                           aagaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGc                                                                                  <  1:333418/140‑1 (MQ=255)                                             gaaaATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTc                                                                                <  2:234560/140‑1 (MQ=255)                                                           tGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATatcat                                                                  <  1:560940/140‑1 (MQ=255)                                                               gTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCttt                                                              <  1:324912/140‑1 (MQ=255)                                                                tATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCtttt                                                             <  1:816932/140‑1 (MQ=255)                                                                    cAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGa                                                         >  1:704772/1‑140 (MQ=255)                                                                     aGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATatc                                                                    >  2:56203/1‑128 (MQ=255)                                                                     aGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATatc                                                                    <  1:56203/128‑1 (MQ=255)                                                                       aCCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCt                                                      >  1:41768/1‑140 (MQ=255)                                                                         cAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATTTTTTAAGCCCTGa                                                    <  2:87251/140‑1 (MQ=255)                                                                          aaGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGAc                                                   >  1:710218/1‑140 (MQ=255)                                                                          aaGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGAc                                                   >  1:590228/1‑140 (MQ=255)                                                                                      aCCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAatat                                       >  1:657154/1‑140 (MQ=255)                                                                                      aCCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAatat                                       >  1:508388/1‑140 (MQ=255)                                                                                                taGTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTcc                             >  2:251139/1‑140 (MQ=255)                                                                                                  gTTATTATCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCaa                           >  2:436738/1‑140 (MQ=255)                                                                                                        atCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAg                     >  2:348077/1‑140 (MQ=255)                                                                                                        atCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAg                     >  1:634496/1‑140 (MQ=255)                                                                                                        atCGGTTATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAg                     >  2:82094/1‑140 (MQ=255)                                                                                                             ttATCTGAATACCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGt                >  1:635515/1‑140 (MQ=255)                                                                                                                       aCCGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaa      >  1:142906/1‑140 (MQ=255)                                                                                                                         cGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaaca    <  1:251139/140‑1 (MQ=255)                                                                                                                         cGGTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaaca    >  2:819240/1‑140 (MQ=255)                                                                                                                         cGGTATAACCCGGCAAATATTTATTAAGGCCGTAGTAGTAGCTTGCTTGAAAAAAATTAGGCTCATCCTAAATATCTTCTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaaca    <  2:635515/140‑1 (MQ=255)                                                                                                                          ggTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaacaa   <  2:704772/140‑1 (MQ=255)                                                                                                                           gTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaacaac  >  1:116312/1‑140 (MQ=255)                                                                                                                           gTATAACCCGGCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGaacaac  >  1:758523/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  TTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAAC  >  NC_000913/154115‑154376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑