Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,561	T→C	F314L (TTC→CTC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,561	0	T	C	98.5%	221.7 / ‑4.5	65	F314L (TTC→CTC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (31/33);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ATCTTTAACTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGAC  >  NC_000913/991429‑991689                                                                                                                                     |                                                                                                                                 atCTTTAACTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCt                                                                                                                           <  1:337794/140‑1 (MQ=255) atCTTTAACTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCt                                                                                                                           <  1:47760/140‑1 (MQ=255)        aCTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTg                                                                                                                    >  1:162360/1‑140 (MQ=255)        aCTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTg                                                                                                                    >  1:57227/1‑140 (MQ=255)          tCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGaa                                                                                                                  >  2:701695/1‑140 (MQ=255)          tCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGaa                                                                                                                  >  2:677214/1‑140 (MQ=255)             aaaTATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAaaga                                                                                                               >  2:41773/1‑140 (MQ=255)              aaTATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAaagaa                                                                                                              >  1:215562/1‑140 (MQ=255)                      cTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAAc                                                                                                      >  2:91675/1‑140 (MQ=255)                        ggCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCg                                                                                                    >  2:562854/1‑140 (MQ=255)                           ccGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCt                                                                                                 >  2:340219/1‑140 (MQ=255)                              cACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTcc                                                                                              <  2:162360/140‑1 (MQ=255)                              cACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTcc                                                                                              <  2:426974/140‑1 (MQ=255)                                   acacCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGAt                                                                                         >  2:399182/1‑140 (MQ=255)                                   acacCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGAt                                                                                         >  2:445217/1‑140 (MQ=255)                                    cacCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATc                                                                                        <  2:57227/140‑1 (MQ=255)                                           aCCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGtt                                                                                 <  1:592191/140‑1 (MQ=255)                                               aCAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCcgcg                                                                                           <  1:294305/126‑1 (MQ=255)                                               aCAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCcgcg                                                                                           >  2:294305/1‑126 (MQ=255)                                               aCAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGc                                                                             <  1:91675/140‑1 (MQ=255)                                                 aaaGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGctc                                                                           <  1:399182/140‑1 (MQ=255)                                                 aaaGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGctc                                                                           <  1:273828/140‑1 (MQ=255)                                                  aaGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGctct                                                                          <  2:488642/140‑1 (MQ=255)                                                   aGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTg                                                                         <  1:340219/140‑1 (MQ=255)                                                    gATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGa                                                                        <  1:677214/140‑1 (MQ=255)                                                    gATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGa                                                                        <  1:701695/140‑1 (MQ=255)                                                       tACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCt                                                                     >  2:391613/1‑140 (MQ=255)                                                                    aaCGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCcgc                                                           >  2:601390/1‑137 (MQ=255)                                                                    aaCGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCcgc                                                           <  1:601390/137‑1 (MQ=255)                                                                        cgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCcg                                                            >  2:510914/1‑132 (MQ=255)                                                                        cgTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCcg                                                            <  1:510914/132‑1 (MQ=255)                                                                         gTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTaa                                                   >  2:38183/1‑140 (MQ=255)                                                                         gTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTaa                                                   >  1:393545/1‑140 (MQ=255)                                                                          ttATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAAc                                                  <  2:174638/140‑1 (MQ=255)                                                                          ttATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAAc                                                  >  1:450623/1‑140 (MQ=255)                                                                          ttATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAAc                                                  <  1:456384/140‑1 (MQ=255)                                                                            aTCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCg                                                <  2:293051/140‑1 (MQ=255)                                                                            aTCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCg                                                <  2:225305/140‑1 (MQ=255)                                                                             tCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGc                                               <  1:41773/140‑1 (MQ=255)                                                                                 ccATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCa                                           <  1:562854/140‑1 (MQ=255)                                                                                  cATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaa                                          <  1:523942/140‑1 (MQ=255)                                                                                   aTGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaat                                         <  1:40743/140‑1 (MQ=255)                                                                                   aTGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaat                                         <  2:215562/140‑1 (MQ=255)                                                                                    tGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaata                                        <  1:445217/140‑1 (MQ=255)                                                                                      aaTATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCaataat                                      <  1:38183/140‑1 (MQ=255)                                                                                                tAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCa                            >  1:708740/1‑140 (MQ=255)                                                                                                tAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCa                            >  1:450599/1‑140 (MQ=255)                                                                                                   cTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgc                         <  2:708740/140‑1 (MQ=255)                                                                                                   cTGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgc                         >  2:643206/1‑140 (MQ=255)                                                                                                    tGGACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgcg                        <  2:450599/140‑1 (MQ=255)                                                                                                     ggACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgcgc                       >  1:525549/1‑140 (MQ=255)                                                                                                     ggACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATgcgc                       >  1:801622/1‑140 (MQ=255)                                                                                                      gACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGcgcg                      >  1:820689/1‑140 (MQ=255)                                                                                                      gACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGcgcg                      >  1:815272/1‑140 (MQ=255)                                                                                                      gACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGcgcg                      >  1:434480/1‑140 (MQ=255)                                                                                                      gACCGGTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGcgcg                      <  2:415165/140‑1 (MQ=255)                                                                                                           gTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTg                 >  2:686082/1‑140 (MQ=255)                                                                                                           gTAACCGAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTg                 <  1:391613/140‑1 (MQ=255)                                                                                                                 gAGTGACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGttt           >  1:436177/1‑140 (MQ=255)                                                                                                                     gACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCag       <  2:434480/140‑1 (MQ=255)                                                                                                                     gACCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCag       <  2:815272/140‑1 (MQ=255)                                                                                                                      aCCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCa               <  2:541509/131‑1 (MQ=255)                                                                                                                      aCCTGTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCa               >  1:541509/1‑131 (MQ=255)                                                                                                                          gTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGAc  >  2:619425/1‑140 (MQ=255)                                                                                                                          gTCGCGACTGGCTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGAc  >  2:78028/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 ATCTTTAACTCCAAATATGTGCTGGCCCGCACCGACACCGCCACCGACAAAGATTACCTCGATATTGAACGCGTTATCGGCCATGAATATTTCCATAACTGGACCGGTAACCGAGTGACCTGTCGCGACTGGTTCCAGCTCAGCCTGAAAGAAGGTTTAACCGTCTTCCGCGATCAGGAGTTCAGCTCTGACCTTGGTTCCCGCGCAGTTAACCGCATCAATAATGTACGCACCATGCGCGGATTGCAGTTTGCAGAAGAC  >  NC_000913/991429‑991689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑